ENSG00000175727

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000319080 ENSG00000175727 HEK293_OSMI2_6hA HEK293_TMG_6hB MLXIP protein_coding protein_coding 21.19515 29.89445 18.05855 2.845279 0.6042268 -0.7268794 16.1263976 23.9539519 11.016810 3.56704055 0.2494869 -1.1198503 0.74820000 0.79316667 0.61076667 -0.18240000 1.413451e-01 2.162223e-06 FALSE TRUE
MSTRG.8206.5 ENSG00000175727 HEK293_OSMI2_6hA HEK293_TMG_6hB MLXIP protein_coding   21.19515 29.89445 18.05855 2.845279 0.6042268 -0.7268794 0.6518779 0.3714082 1.386882 0.09212084 0.1051630 1.8728022 0.03413333 0.01243333 0.07703333 0.06460000 2.162223e-06 2.162223e-06 TRUE TRUE
MSTRG.8206.6 ENSG00000175727 HEK293_OSMI2_6hA HEK293_TMG_6hB MLXIP protein_coding   21.19515 29.89445 18.05855 2.845279 0.6042268 -0.7268794 1.4372393 2.2310967 2.518398 0.31332937 0.2724640 0.1740187 0.07121250 0.07783333 0.13950000 0.06166667 3.726600e-01 2.162223e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000175727 E001 18.6273244 0.0374997614 2.352882e-02 1.076408e-01 12 122078756 122078980 225 + 1.030 1.342 1.109
ENSG00000175727 E002 9.7316394 0.0685026329 2.131716e-02 1.003700e-01 12 122078981 122079029 49 + 0.673 1.092 1.615
ENSG00000175727 E003 12.8179509 0.0131508835 3.952772e-01 6.245782e-01 12 122079030 122079046 17 + 1.030 1.142 0.407
ENSG00000175727 E004 23.9752318 0.0014150165 3.390417e-01 5.771073e-01 12 122079047 122079084 38 + 1.407 1.358 -0.172
ENSG00000175727 E005 113.9181959 0.0003607169 2.684938e-06 6.747029e-05 12 122079085 122079266 182 + 2.125 1.985 -0.470
ENSG00000175727 E006 142.0309545 0.0006809525 1.263016e-03 1.188174e-02 12 122127256 122127362 107 + 2.183 2.098 -0.285
ENSG00000175727 E007 0.0000000       12 122127880 122127882 3 +      
ENSG00000175727 E008 74.4041778 0.0005088003 7.931532e-01 8.922729e-01 12 122127883 122127887 5 + 1.845 1.854 0.032
ENSG00000175727 E009 136.5056997 0.0003720009 5.423939e-01 7.308073e-01 12 122127888 122127968 81 + 2.112 2.113 0.004
ENSG00000175727 E010 2.2168817 0.1944024973 5.297168e-01 7.218534e-01 12 122127969 122129136 1168 + 0.584 0.414 -0.834
ENSG00000175727 E011 114.7540481 0.0006680589 7.082854e-01 8.406942e-01 12 122129137 122129179 43 + 2.031 2.038 0.023
ENSG00000175727 E012 119.3485776 0.0003858360 4.333336e-01 6.536977e-01 12 122129180 122129226 47 + 2.059 2.052 -0.021
ENSG00000175727 E013 107.7788628 0.0005026746 1.273882e-01 3.277228e-01 12 122129588 122129629 42 + 2.035 2.000 -0.117
ENSG00000175727 E014 2.9258505 0.0143737537 2.787288e-01 5.188155e-01 12 122129630 122129940 311 + 0.672 0.512 -0.719
ENSG00000175727 E015 75.1247549 0.0008123222 4.538524e-02 1.685316e-01 12 122129941 122129941 1 + 1.901 1.835 -0.224
ENSG00000175727 E016 184.7837269 0.0008092048 3.991912e-02 1.544902e-01 12 122129942 122130112 171 + 2.272 2.232 -0.134
ENSG00000175727 E017 137.8351626 0.0003133516 3.333175e-02 1.369751e-01 12 122130844 122130933 90 + 2.149 2.102 -0.156
ENSG00000175727 E018 125.4218133 0.0004105690 4.590800e-02 1.698304e-01 12 122132292 122132383 92 + 2.107 2.060 -0.156
ENSG00000175727 E019 3.6693607 0.0791346490 8.185044e-01 9.071743e-01 12 122132525 122133297 773 + 0.670 0.641 -0.126
ENSG00000175727 E020 0.8563349 0.0168785829 5.666124e-01   12 122133298 122133347 50 + 0.165 0.279 0.958
ENSG00000175727 E021 151.2351695 0.0013808472 1.119296e-01 3.023051e-01 12 122133348 122133529 182 + 2.179 2.142 -0.123
ENSG00000175727 E022 69.4568726 0.0021368361 7.326242e-01 8.557662e-01 12 122133530 122133583 54 + 1.818 1.820 0.006
ENSG00000175727 E023 79.4591549 0.0004984786 5.553128e-01 7.399833e-01 12 122133584 122133670 87 + 1.883 1.880 -0.013
ENSG00000175727 E024 216.6562414 0.0003182460 2.001572e-02 9.594203e-02 12 122133671 122133987 317 + 2.338 2.298 -0.132
ENSG00000175727 E025 6.7661488 0.0041373697 6.815451e-01 8.236167e-01 12 122133988 122134164 177 + 0.817 0.889 0.277
ENSG00000175727 E026 14.9271849 0.2432425383 2.175055e-01 4.504181e-01 12 122134321 122135223 903 + 1.292 1.107 -0.653
ENSG00000175727 E027 78.2513566 0.0005461675 9.712014e-03 5.687322e-02 12 122135224 122135234 11 + 1.931 1.843 -0.294
ENSG00000175727 E028 73.5105057 0.0006624108 1.718861e-03 1.515194e-02 12 122135235 122135235 1 + 1.921 1.807 -0.386
ENSG00000175727 E029 143.6967128 0.0024709041 3.961253e-03 2.893135e-02 12 122135236 122135345 110 + 2.194 2.101 -0.309
ENSG00000175727 E030 1.3080092 0.0468999712 3.088128e-01   12 122135454 122135488 35 + 0.456 0.279 -1.045
ENSG00000175727 E031 140.9208796 0.0045673784 7.145040e-02 2.276960e-01 12 122135489 122135627 139 + 2.166 2.105 -0.206
ENSG00000175727 E032 79.3413087 0.0067689450 8.351002e-02 2.517829e-01 12 122135628 122135666 39 + 1.930 1.852 -0.263
ENSG00000175727 E033 37.8704704 0.0692392832 6.222501e-02 2.077874e-01 12 122135667 122137293 1627 + 1.699 1.474 -0.767
ENSG00000175727 E034 9.4388496 0.1386245845 2.505984e-01 4.886233e-01 12 122137294 122137468 175 + 1.101 0.932 -0.621
ENSG00000175727 E035 135.8167171 0.0051162037 2.429610e-02 1.100101e-01 12 122137469 122137590 122 + 2.165 2.077 -0.296
ENSG00000175727 E036 11.5965692 0.0079981048 2.717198e-01 5.115721e-01 12 122138191 122138193 3 + 1.131 1.029 -0.367
ENSG00000175727 E037 145.3341617 0.0033735748 5.860342e-02 1.996545e-01 12 122138194 122138295 102 + 2.177 2.116 -0.201
ENSG00000175727 E038 7.6059216 0.3389963449 8.383671e-01 9.185479e-01 12 122138296 122138423 128 + 0.830 0.943 0.431
ENSG00000175727 E039 155.9312414 0.0087507051 3.694655e-01 6.035943e-01 12 122138424 122138551 128 + 2.190 2.155 -0.115
ENSG00000175727 E040 130.3439771 0.0004620488 8.692885e-01 9.355092e-01 12 122138815 122138938 124 + 2.076 2.103 0.091
ENSG00000175727 E041 1.1547758 0.0237190273 8.569582e-01   12 122138939 122138941 3 + 0.285 0.328 0.283
ENSG00000175727 E042 5.2766683 0.0516299425 6.106455e-01 7.773629e-01 12 122140827 122140953 127 + 0.817 0.756 -0.244
ENSG00000175727 E043 142.2377019 0.0003731209 5.267341e-02 1.859069e-01 12 122140954 122141083 130 + 2.156 2.116 -0.135
ENSG00000175727 E044 1193.2536829 0.0006667230 9.332650e-01 9.687790e-01 12 122141691 122143415 1725 + 3.037 3.053 0.053
ENSG00000175727 E045 2236.6000669 0.0006281433 1.556185e-14 2.226420e-12 12 122143416 122147062 3647 + 3.249 3.352 0.342
ENSG00000175727 E046 263.7048613 0.0096957519 4.085666e-03 2.965192e-02 12 122147063 122147344 282 + 2.267 2.445 0.592