ENSG00000175548

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000308742 ENSG00000175548 HEK293_OSMI2_6hA HEK293_TMG_6hB ALG10B protein_coding protein_coding 5.416129 1.282239 9.607205 0.08373563 0.4398193 2.895744 2.5645586 0.56425193 4.6335686 0.09036659 0.1289065 3.015478 0.47766250 0.44413333 0.48450000 0.04036667 0.9417435100 0.0002703547 FALSE TRUE
ENST00000548240 ENSG00000175548 HEK293_OSMI2_6hA HEK293_TMG_6hB ALG10B protein_coding nonsense_mediated_decay 5.416129 1.282239 9.607205 0.08373563 0.4398193 2.895744 0.2954348 0.00000000 1.1361331 0.00000000 0.1012512 6.840631 0.03409583 0.00000000 0.11786667 0.11786667 0.0002703547 0.0002703547 FALSE TRUE
ENST00000551464 ENSG00000175548 HEK293_OSMI2_6hA HEK293_TMG_6hB ALG10B protein_coding protein_coding 5.416129 1.282239 9.607205 0.08373563 0.4398193 2.895744 2.1420331 0.62953821 3.2920401 0.08943191 0.3765274 2.368255 0.38197917 0.49316667 0.34050000 -0.15266667 0.5193744803 0.0002703547 FALSE TRUE
MSTRG.7109.3 ENSG00000175548 HEK293_OSMI2_6hA HEK293_TMG_6hB ALG10B protein_coding   5.416129 1.282239 9.607205 0.08373563 0.4398193 2.895744 0.2234406 0.07016125 0.2386118 0.07016125 0.1383181 1.632918 0.07170000 0.04843333 0.02483333 -0.02360000 1.0000000000 0.0002703547 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000175548 E001 0.3336024 0.0274424043 6.475068e-03   12 38316762 38316766 5 + 0.000 0.432 12.070
ENSG00000175548 E002 1.0289696 0.0603778442 2.029144e-01   12 38316767 38316770 4 + 0.174 0.432 1.788
ENSG00000175548 E003 1.0407361 0.0173157905 7.684135e-01   12 38316771 38316773 3 + 0.208 0.268 0.475
ENSG00000175548 E004 17.4553297 0.0020361775 6.908400e-01 8.295942e-01 12 38316774 38316838 65 + 1.093 1.139 0.165
ENSG00000175548 E005 37.1616205 0.0010791090 3.264447e-01 5.656458e-01 12 38316839 38317038 200 + 1.398 1.476 0.269
ENSG00000175548 E006 21.0769953 0.0017575140 2.397927e-01 4.763958e-01 12 38317039 38317064 26 + 1.157 1.276 0.421
ENSG00000175548 E007 4.8442843 0.0052178235 6.589460e-01 8.093727e-01 12 38317065 38318260 1196 + 0.634 0.550 -0.373
ENSG00000175548 E008 40.5107841 0.0018217850 9.802210e-01 9.919203e-01 12 38318261 38318458 198 + 1.448 1.450 0.008
ENSG00000175548 E009 290.9590161 0.0002915447 2.384298e-11 1.921731e-09 12 38320161 38323912 3752 + 2.311 2.123 -0.628
ENSG00000175548 E010 21.6418926 0.0021988462 6.370873e-01 7.951054e-01 12 38323913 38324052 140 + 1.185 1.234 0.175
ENSG00000175548 E011 330.5441607 0.0002216027 4.375191e-07 1.376961e-05 12 38324053 38329721 5669 + 2.331 2.435 0.346