ENSG00000174672

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000308219 ENSG00000174672 HEK293_OSMI2_6hA HEK293_TMG_6hB BRSK2 protein_coding protein_coding 23.92249 34.22419 14.81824 1.388678 0.9244264 -1.207091 3.4701356 4.951868 0.83782899 1.1755085 0.29115723 -2.5490381 0.13952500 0.14263333 0.055266667 -0.08736667 2.601521e-01 4.982579e-05 FALSE TRUE
ENST00000526678 ENSG00000174672 HEK293_OSMI2_6hA HEK293_TMG_6hB BRSK2 protein_coding protein_coding 23.92249 34.22419 14.81824 1.388678 0.9244264 -1.207091 6.4936689 8.814556 6.29301805 0.8283373 0.08922343 -0.4854809 0.28755000 0.25680000 0.428466667 0.17166667 2.517481e-02 4.982579e-05 FALSE TRUE
ENST00000526768 ENSG00000174672 HEK293_OSMI2_6hA HEK293_TMG_6hB BRSK2 protein_coding retained_intron 23.92249 34.22419 14.81824 1.388678 0.9244264 -1.207091 1.7303602 2.792032 1.83359410 0.7524119 0.11605843 -0.6039525 0.07571250 0.08306667 0.124800000 0.04173333 6.339261e-01 4.982579e-05 TRUE FALSE
ENST00000529951 ENSG00000174672 HEK293_OSMI2_6hA HEK293_TMG_6hB BRSK2 protein_coding nonsense_mediated_decay 23.92249 34.22419 14.81824 1.388678 0.9244264 -1.207091 1.0050976 2.681244 0.03520257 0.5737568 0.03520257 -5.8957243 0.03604583 0.07730000 0.002633333 -0.07466667 4.982579e-05 4.982579e-05 TRUE TRUE
ENST00000531078 ENSG00000174672 HEK293_OSMI2_6hA HEK293_TMG_6hB BRSK2 protein_coding retained_intron 23.92249 34.22419 14.81824 1.388678 0.9244264 -1.207091 0.4082176 0.000000 0.94855618 0.0000000 0.49244150 6.5827911 0.02262917 0.00000000 0.062000000 0.06200000 1.740346e-01 4.982579e-05 TRUE TRUE
ENST00000531932 ENSG00000174672 HEK293_OSMI2_6hA HEK293_TMG_6hB BRSK2 protein_coding retained_intron 23.92249 34.22419 14.81824 1.388678 0.9244264 -1.207091 3.0135368 3.629104 2.09829004 1.3799041 0.23263027 -0.7875101 0.12864583 0.10790000 0.141033333 0.03313333 7.851767e-01 4.982579e-05 FALSE FALSE
MSTRG.4940.7 ENSG00000174672 HEK293_OSMI2_6hA HEK293_TMG_6hB BRSK2 protein_coding   23.92249 34.22419 14.81824 1.388678 0.9244264 -1.207091 3.5656833 5.617620 1.47339767 0.4944721 0.25691896 -1.9236195 0.14154167 0.16423333 0.101500000 -0.06273333 4.396644e-01 4.982579e-05 FALSE TRUE
MSTRG.4940.9 ENSG00000174672 HEK293_OSMI2_6hA HEK293_TMG_6hB BRSK2 protein_coding   23.92249 34.22419 14.81824 1.388678 0.9244264 -1.207091 2.2017518 3.285538 1.07324680 0.1675495 0.43285127 -1.6051519 0.09062083 0.09666667 0.069433333 -0.02723333 7.606379e-01 4.982579e-05 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000174672 E001 0.0000000       11 1389867 1389898 32 +      
ENSG00000174672 E002 0.0000000       11 1389899 1389900 2 +      
ENSG00000174672 E003 0.0000000       11 1389901 1389933 33 +      
ENSG00000174672 E004 2.8260593 0.0087309641 2.129411e-01 4.452246e-01 11 1389934 1390201 268 + 0.341 0.578 1.222
ENSG00000174672 E005 18.2670569 0.0019351024 2.237126e-02 1.038103e-01 11 1390202 1390284 83 + 1.023 1.257 0.838
ENSG00000174672 E006 50.5680246 0.0009419325 3.800411e-01 6.121933e-01 11 1390285 1390375 91 + 1.593 1.643 0.168
ENSG00000174672 E007 0.0000000       11 1409400 1409631 232 +      
ENSG00000174672 E008 0.1723744 0.0337104090 1.000000e+00   11 1409759 1409859 101 + 0.000 0.070 8.593
ENSG00000174672 E009 0.3032425 0.0244411696 8.517676e-01   11 1410443 1410906 464 + 0.000 0.130 9.702
ENSG00000174672 E010 0.0000000       11 1411152 1411633 482 +      
ENSG00000174672 E011 98.3734068 0.0003914104 5.866979e-01 7.613905e-01 11 1436040 1436134 95 + 1.939 1.913 -0.087
ENSG00000174672 E012 117.5717824 0.0004184333 1.116028e-01 3.017044e-01 11 1438306 1438391 86 + 2.045 1.981 -0.212
ENSG00000174672 E013 79.7085151 0.0006009291 1.330430e-01 3.365873e-01 11 1440788 1440798 11 + 1.885 1.814 -0.241
ENSG00000174672 E014 163.7508766 0.0003180252 9.242781e-04 9.294392e-03 11 1440799 1440928 130 + 2.222 2.115 -0.358
ENSG00000174672 E015 0.1614157 0.0344910304 1.324589e-01   11 1442445 1442489 45 + 0.203 0.000 -11.479
ENSG00000174672 E016 183.3023458 0.0015248429 2.978392e-03 2.326584e-02 11 1442490 1442606 117 + 2.269 2.166 -0.346
ENSG00000174672 E017 123.6590141 0.0013938821 2.158399e-01 4.484674e-01 11 1443106 1443139 34 + 2.061 2.009 -0.174
ENSG00000174672 E018 148.6682499 0.0049423163 2.349444e-02 1.075299e-01 11 1443335 1443403 69 + 2.179 2.073 -0.355
ENSG00000174672 E019 165.8599738 0.0022440206 2.846697e-03 2.248011e-02 11 1443489 1443635 147 + 2.232 2.119 -0.378
ENSG00000174672 E020 71.2690896 0.0005599101 1.295406e-01 3.310966e-01 11 1444971 1445002 32 + 1.843 1.767 -0.255
ENSG00000174672 E021 136.6091132 0.0003795709 1.596089e-01 3.762060e-01 11 1445294 1445458 165 + 2.103 2.051 -0.177
ENSG00000174672 E022 114.3823885 0.0003977074 2.930596e-01 5.337708e-01 11 1445571 1445668 98 + 2.020 1.977 -0.147
ENSG00000174672 E023 137.1978474 0.0015294795 2.786825e-01 5.187827e-01 11 1445757 1445907 151 + 2.034 2.074 0.132
ENSG00000174672 E024 42.2741393 0.0025755131 4.000047e-01 6.282428e-01 11 1447798 1447863 66 + 1.600 1.541 -0.203
ENSG00000174672 E025 84.6663033 0.0009103301 6.061219e-03 4.003441e-02 11 1449776 1449836 61 + 1.752 1.884 0.446
ENSG00000174672 E026 102.1956562 0.0004269913 2.025613e-02 9.677209e-02 11 1450587 1450657 71 + 1.857 1.955 0.327
ENSG00000174672 E027 95.1540000 0.0041401259 2.019272e-01 4.319991e-01 11 1450658 1450684 27 + 1.851 1.914 0.214
ENSG00000174672 E028 162.7882850 0.0010273401 1.931214e-02 9.354649e-02 11 1450685 1450794 110 + 2.070 2.152 0.274
ENSG00000174672 E029 144.2293099 0.0003973976 7.036786e-01 8.379248e-01 11 1451371 1451419 49 + 2.095 2.078 -0.057
ENSG00000174672 E030 55.9219117 0.0969315958 3.234080e-02 1.342084e-01 11 1453838 1454426 589 + 1.883 1.592 -0.984
ENSG00000174672 E031 22.1931105 0.1126065866 8.363503e-02 2.520658e-01 11 1454427 1454484 58 + 1.472 1.216 -0.893
ENSG00000174672 E032 231.8337747 0.0010709509 4.014163e-01 6.294933e-01 11 1454485 1454608 124 + 2.309 2.279 -0.100
ENSG00000174672 E033 294.2973848 0.0013618544 6.980429e-01 8.342980e-01 11 1456348 1456528 181 + 2.402 2.386 -0.052
ENSG00000174672 E034 176.0134763 0.0073157974 2.218649e-01 4.557500e-01 11 1456598 1456687 90 + 2.128 2.180 0.175
ENSG00000174672 E035 63.8656204 0.0023057423 3.402477e-01 5.781831e-01 11 1456955 1457049 95 + 1.778 1.721 -0.193
ENSG00000174672 E036 111.6619947 0.0042950542 2.413758e-01 4.781425e-01 11 1459192 1459236 45 + 1.934 1.987 0.177
ENSG00000174672 E037 68.1063387 0.0011100367 1.204467e-01 3.165517e-01 11 1459237 1459239 3 + 1.703 1.783 0.270
ENSG00000174672 E038 18.6496193 0.1409688292 2.226995e-01 4.566549e-01 11 1459240 1459447 208 + 1.342 1.173 -0.592
ENSG00000174672 E039 7.7375867 0.0327155526 1.398651e-01 3.472713e-01 11 1460500 1460547 48 + 1.023 0.809 -0.810
ENSG00000174672 E040 90.7183428 0.0040770774 7.561461e-01 8.703116e-01 11 1460548 1460969 422 + 1.872 1.883 0.038
ENSG00000174672 E041 528.0676687 0.0008086822 1.907466e-16 3.517172e-14 11 1460970 1462689 1720 + 2.498 2.680 0.606