ENSG00000174327

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000308027 ENSG00000174327 HEK293_OSMI2_6hA HEK293_TMG_6hB SLC16A13 protein_coding protein_coding 4.43036 7.523317 1.766419 0.1418446 0.1690808 -2.084313 4.1577721 6.9704741 1.766419 0.10439171 0.1690808 -1.974353 0.94725417 0.92663333 1 0.07336667 0.002324285 0.002324285 FALSE  
ENST00000575844 ENSG00000174327 HEK293_OSMI2_6hA HEK293_TMG_6hB SLC16A13 protein_coding retained_intron 4.43036 7.523317 1.766419 0.1418446 0.1690808 -2.084313 0.2725876 0.5528429 0.000000 0.03749748 0.0000000 -5.814660 0.05274583 0.07336667 0 -0.07336667 0.002464377 0.002324285 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000174327 E001 18.667722 0.051187670 0.805441206 0.89935688 17 7036015 7036096 82 + 1.116 1.167 0.183
ENSG00000174327 E002 58.978667 0.272594335 0.322230045 0.56164166 17 7036097 7036581 485 + 1.507 1.654 0.499
ENSG00000174327 E003 4.117962 0.006314933 0.009458033 0.05577635 17 7036582 7036726 145 + 0.000 0.645 12.486
ENSG00000174327 E004 28.725507 0.001530585 0.172903676 0.39434624 17 7036727 7036870 144 + 1.210 1.352 0.497
ENSG00000174327 E005 2.185135 0.010689250 0.085519138 0.25555819 17 7036871 7037064 194 + 0.000 0.444 11.544
ENSG00000174327 E006 110.832518 0.010847848 0.126137383 0.32571585 17 7038152 7038889 738 + 1.943 1.895 -0.160
ENSG00000174327 E007 66.130109 0.013037882 0.211972914 0.44405693 17 7039763 7040117 355 + 1.730 1.672 -0.194