ENSG00000173875

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000343325 ENSG00000173875 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF791 protein_coding protein_coding 6.728679 4.313755 9.85318 1.023449 1.073854 1.189768 3.8097857 1.1701506 7.7140500 0.06448877 0.24977662 2.7103866 0.5305375 0.30010000 0.7960333 0.49593333 0.002166708 0.002166708 FALSE TRUE
ENST00000498389 ENSG00000173875 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF791 protein_coding processed_transcript 6.728679 4.313755 9.85318 1.023449 1.073854 1.189768 2.3831348 2.8599655 1.4187885 0.98867980 0.96065727 -1.0062410 0.3879708 0.62820000 0.1275333 -0.50066667 0.247392216 0.002166708   FALSE
ENST00000597691 ENSG00000173875 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF791 protein_coding nonsense_mediated_decay 6.728679 4.313755 9.85318 1.023449 1.073854 1.189768 0.3702637 0.2836392 0.5225764 0.05692983 0.05747174 0.8589441 0.0577250 0.07173333 0.0551000 -0.01663333 0.873215974 0.002166708 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000173875 E001 0.000000       19 12610918 12610919 2 +      
ENSG00000173875 E002 15.549619 0.0040487035 1.679009e-02 8.469254e-02 19 12610920 12610953 34 + 1.078 1.280 0.719
ENSG00000173875 E003 18.212779 0.0022327278 4.984145e-03 3.453892e-02 19 12610954 12610957 4 + 1.143 1.360 0.766
ENSG00000173875 E004 47.369728 0.0012191394 8.975713e-05 1.364988e-03 19 12610958 12611082 125 + 1.552 1.742 0.645
ENSG00000173875 E005 10.477869 0.0362448441 7.133469e-08 2.745133e-06 19 12612226 12612316 91 + 0.740 1.419 2.487
ENSG00000173875 E006 6.238839 0.0311593619 1.003032e-05 2.102417e-04 19 12612317 12612365 49 + 0.590 1.179 2.289
ENSG00000173875 E007 2.122721 0.0296824347 4.080757e-04 4.808871e-03 19 12612366 12612394 29 + 0.256 0.831 2.847
ENSG00000173875 E008 54.157965 0.0009394351 6.546282e-02 2.149393e-01 19 12623700 12623826 127 + 1.673 1.522 -0.514
ENSG00000173875 E009 42.747858 0.0012666336 1.604348e-02 8.196852e-02 19 12624650 12624710 61 + 1.583 1.373 -0.722
ENSG00000173875 E010 689.335845 0.0041727100 4.923369e-05 8.208338e-04 19 12627721 12633840 6120 + 2.761 2.677 -0.282