ENSG00000173295

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000310542 ENSG00000173295 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM86B3P transcribed_unprocessed_pseudogene processed_transcript 4.50867 4.952144 3.630384 0.5784314 0.1990189 -0.4468727 1.41055879 1.4762433 1.2070513 0.34544719 0.06298269 -0.288280359 0.31163333 0.29063333 0.33516667 0.04453333 0.877395814 0.008200204 TRUE  
ENST00000523992 ENSG00000173295 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM86B3P transcribed_unprocessed_pseudogene transcribed_unprocessed_pseudogene 4.50867 4.952144 3.630384 0.5784314 0.1990189 -0.4468727 0.09055299 0.0000000 0.2061031 0.00000000 0.20610307 4.433647663 0.02092917 0.00000000 0.05313333 0.05313333 0.831400114 0.008200204 TRUE  
ENST00000587202 ENSG00000173295 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM86B3P transcribed_unprocessed_pseudogene processed_transcript 4.50867 4.952144 3.630384 0.5784314 0.1990189 -0.4468727 0.31903208 0.4166919 0.0000000 0.15419442 0.00000000 -5.415122976 0.06925417 0.09126667 0.00000000 -0.09126667 0.008200204 0.008200204    
ENST00000588728 ENSG00000173295 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM86B3P transcribed_unprocessed_pseudogene processed_transcript 4.50867 4.952144 3.630384 0.5784314 0.1990189 -0.4468727 0.24089846 0.2994718 0.1403551 0.02586368 0.07082360 -1.041434217 0.05745000 0.06120000 0.03670000 -0.02450000 0.766413807 0.008200204    
ENST00000590591 ENSG00000173295 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM86B3P transcribed_unprocessed_pseudogene processed_transcript 4.50867 4.952144 3.630384 0.5784314 0.1990189 -0.4468727 0.82140410 0.8795589 0.8755841 0.28840980 0.17552757 -0.006460812 0.18191667 0.17116667 0.24416667 0.07300000 0.786035505 0.008200204    
MSTRG.31089.11 ENSG00000173295 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM86B3P transcribed_unprocessed_pseudogene   4.50867 4.952144 3.630384 0.5784314 0.1990189 -0.4468727 0.18981886 0.2099087 0.3983633 0.20990866 0.23256627 0.892948952 0.04532917 0.03503333 0.11736667 0.08233333 0.731061224 0.008200204 TRUE  
MSTRG.31089.15 ENSG00000173295 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM86B3P transcribed_unprocessed_pseudogene   4.50867 4.952144 3.630384 0.5784314 0.1990189 -0.4468727 0.23934560 0.1693652 0.1024326 0.08684772 0.05401765 -0.673839922 0.05304583 0.03193333 0.02983333 -0.00210000 1.000000000 0.008200204 FALSE  
MSTRG.31089.17 ENSG00000173295 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM86B3P transcribed_unprocessed_pseudogene   4.50867 4.952144 3.630384 0.5784314 0.1990189 -0.4468727 0.35237893 0.4754450 0.4896093 0.13088299 0.26400199 0.041492363 0.07210833 0.10256667 0.12753333 0.02496667 1.000000000 0.008200204    
MSTRG.31089.8 ENSG00000173295 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM86B3P transcribed_unprocessed_pseudogene   4.50867 4.952144 3.630384 0.5784314 0.1990189 -0.4468727 0.22887977 0.2690899 0.0000000 0.15062267 0.00000000 -4.802658039 0.05082083 0.06306667 0.00000000 -0.06306667 0.450093475 0.008200204 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000173295 E001 0.1426347 0.031624768 0.2437073031   8 8228595 8228595 1 + 0.169 0.000 -13.777
ENSG00000173295 E002 0.1426347 0.031624768 0.2437073031   8 8228596 8228599 4 + 0.169 0.000 -13.779
ENSG00000173295 E003 0.1426347 0.031624768 0.2437073031   8 8228600 8228604 5 + 0.169 0.000 -13.779
ENSG00000173295 E004 0.1426347 0.031624768 0.2437073031   8 8228605 8228608 4 + 0.169 0.000 -13.779
ENSG00000173295 E005 0.1426347 0.031624768 0.2437073031   8 8228609 8228626 18 + 0.169 0.000 -13.779
ENSG00000173295 E006 0.0000000       8 8228627 8228644 18 +      
ENSG00000173295 E007 0.0000000       8 8228645 8228647 3 +      
ENSG00000173295 E008 0.1308682 0.032305286 1.0000000000   8 8228648 8228652 5 + 0.000 0.086 11.507
ENSG00000173295 E009 1.0598092 0.413970032 0.1143443176   8 8228653 8228700 48 + 0.000 0.393 14.261
ENSG00000173295 E010 1.0598092 0.413970032 0.1143443176   8 8228701 8228713 13 + 0.000 0.393 14.261
ENSG00000173295 E011 1.2321836 0.117924877 0.0359964186   8 8228714 8228727 14 + 0.000 0.435 14.489
ENSG00000173295 E012 1.5769323 0.013696863 0.0097315967 0.056964323 8 8228728 8228797 70 + 0.000 0.503 14.830
ENSG00000173295 E013 2.2426070 0.150689481 0.0627413480 0.208915981 8 8230843 8230905 63 + 0.169 0.598 2.637
ENSG00000173295 E014 5.6425831 0.005435729 0.3720791578 0.605653513 8 8232794 8232874 81 + 0.722 0.826 0.413
ENSG00000173295 E015 5.3345389 0.009148295 0.7647502629 0.875491660 8 8234483 8234584 102 + 0.760 0.781 0.087
ENSG00000173295 E016 9.8173122 0.061444351 0.6432834194 0.799025176 8 8235816 8235949 134 + 0.996 1.016 0.073
ENSG00000173295 E017 8.9854785 0.063363533 0.5239450516 0.717779959 8 8236032 8236299 268 + 0.928 0.993 0.240
ENSG00000173295 E018 9.3210359 0.004158902 0.1755907765 0.397922807 8 8237126 8237207 82 + 0.884 1.018 0.505
ENSG00000173295 E019 9.7116878 0.003444746 0.4503140392 0.665955614 8 8237208 8237275 68 + 0.958 1.018 0.222
ENSG00000173295 E020 11.8094877 0.023114361 0.1768088769 0.399488026 8 8238293 8238926 634 + 1.194 1.012 -0.656
ENSG00000173295 E021 9.7328898 0.091062396 0.9577166806 0.980699250 8 8239627 8240030 404 + 1.050 0.982 -0.250
ENSG00000173295 E022 30.0078973 0.034061150 0.8275202893 0.912360104 8 8240369 8241274 906 + 1.480 1.452 -0.095
ENSG00000173295 E023 13.7754326 0.070361037 0.9278347249 0.965973568 8 8241275 8241540 266 + 1.152 1.136 -0.058
ENSG00000173295 E024 10.4718152 0.037964057 0.3494385959 0.586741545 8 8241541 8241627 87 + 0.958 1.060 0.377
ENSG00000173295 E025 8.5767382 0.005961428 0.4892583669 0.693492393 8 8241628 8241680 53 + 0.910 0.969 0.221
ENSG00000173295 E026 9.5261395 0.011789292 0.7564895088 0.870535781 8 8241681 8241818 138 + 1.022 0.961 -0.223
ENSG00000173295 E027 9.1722131 0.094816600 0.8815529979 0.942140280 8 8241819 8241845 27 + 0.984 0.965 -0.070
ENSG00000173295 E028 16.3013642 0.108222280 0.1481284438 0.359653676 8 8241846 8241962 117 + 1.386 1.087 -1.058
ENSG00000173295 E029 18.7051831 0.047643653 0.0021806451 0.018275858 8 8241963 8242311 349 + 1.487 1.097 -1.369
ENSG00000173295 E030 3.6387136 0.038196166 0.0823439942 0.249380364 8 8242312 8242315 4 + 0.827 0.530 -1.259
ENSG00000173295 E031 9.1852531 0.006139386 0.0274190554 0.119553924 8 8242316 8242432 117 + 1.141 0.879 -0.968
ENSG00000173295 E032 20.1298840 0.002073291 0.2239142401 0.458108908 8 8244099 8244322 224 + 1.246 1.320 0.260
ENSG00000173295 E033 17.2971813 0.002182825 0.0008632309 0.008797724 8 8244323 8244584 262 + 1.021 1.307 1.019
ENSG00000173295 E034 12.6587965 0.003486006 0.0174296207 0.087031769 8 8244585 8244865 281 + 0.934 1.164 0.839