ENSG00000173226

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000310864 ENSG00000173226 HEK293_OSMI2_6hA HEK293_TMG_6hB IQCB1 protein_coding protein_coding 26.67198 13.4811 36.80301 2.217529 0.8951606 1.448208 6.552804 2.8234037 7.9753568 0.7885664 0.24134005 1.4948207 0.28082083 0.20570000 0.21670000 0.011000000 9.592036e-01 2.775876e-08 FALSE TRUE
ENST00000349820 ENSG00000173226 HEK293_OSMI2_6hA HEK293_TMG_6hB IQCB1 protein_coding protein_coding 26.67198 13.4811 36.80301 2.217529 0.8951606 1.448208 3.307491 2.5017668 5.0931334 0.3688788 0.61800320 1.0226809 0.12762500 0.18670000 0.13810000 -0.048600000 5.086304e-01 2.775876e-08 FALSE TRUE
ENST00000393650 ENSG00000173226 HEK293_OSMI2_6hA HEK293_TMG_6hB IQCB1 protein_coding nonsense_mediated_decay 26.67198 13.4811 36.80301 2.217529 0.8951606 1.448208 1.635079 0.7127601 1.8403218 0.6542143 0.09573342 1.3561874 0.05408333 0.04250000 0.05013333 0.007633333 7.380731e-01 2.775876e-08 TRUE TRUE
ENST00000471726 ENSG00000173226 HEK293_OSMI2_6hA HEK293_TMG_6hB IQCB1 protein_coding retained_intron 26.67198 13.4811 36.80301 2.217529 0.8951606 1.448208 2.320123 0.9927218 4.4647441 0.4718394 0.42747636 2.1578838 0.08153333 0.08873333 0.12190000 0.033166667 7.380731e-01 2.775876e-08   FALSE
MSTRG.23530.4 ENSG00000173226 HEK293_OSMI2_6hA HEK293_TMG_6hB IQCB1 protein_coding   26.67198 13.4811 36.80301 2.217529 0.8951606 1.448208 3.212617 1.6315395 4.5136330 0.1716998 0.90858898 1.4624324 0.12274167 0.12846667 0.12170000 -0.006766667 9.999435e-01 2.775876e-08 TRUE TRUE
MSTRG.23530.6 ENSG00000173226 HEK293_OSMI2_6hA HEK293_TMG_6hB IQCB1 protein_coding   26.67198 13.4811 36.80301 2.217529 0.8951606 1.448208 1.693333 1.1119986 0.8157264 0.2469348 0.40861045 -0.4423351 0.07008750 0.08246667 0.02250000 -0.059966667 5.420896e-01 2.775876e-08 FALSE TRUE
MSTRG.23530.7 ENSG00000173226 HEK293_OSMI2_6hA HEK293_TMG_6hB IQCB1 protein_coding   26.67198 13.4811 36.80301 2.217529 0.8951606 1.448208 1.085419 0.0000000 2.2065966 0.0000000 0.46124755 7.7922024 0.02635833 0.00000000 0.05940000 0.059400000 2.775876e-08 2.775876e-08 FALSE TRUE
MSTRG.23530.9 ENSG00000173226 HEK293_OSMI2_6hA HEK293_TMG_6hB IQCB1 protein_coding   26.67198 13.4811 36.80301 2.217529 0.8951606 1.448208 5.714788 3.1566319 7.4813107 0.8330320 1.16545659 1.2422690 0.19810417 0.22613333 0.20380000 -0.022333333 9.292809e-01 2.775876e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000173226 E001 22.894143 0.0056054641 6.019535e-01 0.7714468096 3 121769761 121769993 233 - 1.335 1.380 0.156
ENSG00000173226 E002 44.239988 0.0009199759 4.129735e-02 0.1581089744 3 121769994 121770098 105 - 1.593 1.709 0.393
ENSG00000173226 E003 48.559759 0.0037251316 1.309798e-03 0.0122344142 3 121770099 121770138 40 - 1.604 1.793 0.639
ENSG00000173226 E004 90.590751 0.0032559287 2.333167e-03 0.0192707307 3 121770139 121770212 74 - 1.889 2.029 0.471
ENSG00000173226 E005 71.814076 0.0008907290 1.163356e-01 0.3096789412 3 121770213 121770217 5 - 1.815 1.888 0.246
ENSG00000173226 E006 71.286519 0.0009428994 6.399192e-02 0.2116641886 3 121770218 121770219 2 - 1.808 1.893 0.289
ENSG00000173226 E007 266.827137 0.0022783978 1.419923e-04 0.0020044525 3 121770220 121770574 355 - 2.363 2.476 0.380
ENSG00000173226 E008 176.531630 0.0004120260 1.281507e-01 0.3288734623 3 121772557 121772713 157 - 2.207 2.254 0.157
ENSG00000173226 E009 182.801556 0.0002899782 2.339116e-01 0.4696512693 3 121781743 121781874 132 - 2.230 2.267 0.125
ENSG00000173226 E010 165.447341 0.0059109090 5.256654e-01 0.7189740699 3 121788284 121788432 149 - 2.183 2.223 0.133
ENSG00000173226 E011 183.264902 0.0003419059 2.285451e-01 0.4635736642 3 121790073 121790215 143 - 2.229 2.266 0.126
ENSG00000173226 E012 65.606600 0.0040902981 7.675001e-01 0.8771081994 3 121795457 121795460 4 - 1.790 1.807 0.057
ENSG00000173226 E013 126.651176 0.0032450598 4.335373e-01 0.6539136645 3 121795461 121795566 106 - 2.088 2.056 -0.108
ENSG00000173226 E014 107.473431 0.0004684586 3.864992e-01 0.6177141712 3 121797118 121797187 70 - 2.018 1.991 -0.091
ENSG00000173226 E015 84.455215 0.0030923558 2.762038e-01 0.5162036706 3 121797188 121797227 40 - 1.923 1.875 -0.159
ENSG00000173226 E016 137.008270 0.0029661176 1.544881e-01 0.3687318089 3 121799196 121799374 179 - 2.132 2.080 -0.173
ENSG00000173226 E017 121.130110 0.0005137791 1.556831e-02 0.0802384425 3 121807344 121807443 100 - 2.087 2.007 -0.269
ENSG00000173226 E018 87.475515 0.0004503029 7.170585e-03 0.0454393903 3 121808916 121809009 94 - 1.953 1.847 -0.357
ENSG00000173226 E019 1.035197 0.1036814698 8.894936e-01   3 121811816 121811865 50 - 0.322 0.286 -0.232
ENSG00000173226 E020 120.908597 0.0003843292 3.547569e-03 0.0266339005 3 121826051 121826180 130 - 2.092 1.995 -0.324
ENSG00000173226 E021 80.349810 0.0005201400 6.123867e-04 0.0066892226 3 121828470 121828532 63 - 1.929 1.785 -0.485
ENSG00000173226 E022 87.716655 0.0004252996 1.643283e-05 0.0003231442 3 121828533 121828632 100 - 1.974 1.798 -0.592
ENSG00000173226 E023 88.036903 0.0004637391 5.049035e-02 0.1808110726 3 121828861 121828972 112 - 1.948 1.873 -0.253
ENSG00000173226 E024 30.088732 0.1575547343 2.058799e-01 0.4369120417 3 121833936 121834248 313 - 1.527 1.301 -0.779
ENSG00000173226 E025 17.064489 0.2702620667 4.596043e-01 0.6728335028 3 121834249 121834390 142 - 1.262 1.153 -0.387
ENSG00000173226 E026 69.660687 0.0006009667 2.500447e-01 0.4879945500 3 121834391 121834479 89 - 1.837 1.789 -0.164
ENSG00000173226 E027 46.990517 0.0009143692 1.146782e-01 0.3069076319 3 121834966 121835079 114 - 1.680 1.596 -0.286