ENSG00000173214

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000658925 ENSG00000173214 HEK293_OSMI2_6hA HEK293_TMG_6hB MFSD4B protein_coding processed_transcript 3.731786 2.363912 4.142418 0.4247419 0.1701059 0.8066855 0.3879691 0.65328735 0.04682431 0.22458299 0.04682431 -3.5450539 0.11108333 0.25710000 0.01063333 -0.24646667 0.006368319 0.006368319   FALSE
ENST00000671876 ENSG00000173214 HEK293_OSMI2_6hA HEK293_TMG_6hB MFSD4B protein_coding protein_coding 3.731786 2.363912 4.142418 0.4247419 0.1701059 0.8066855 0.9942492 0.69316699 1.35693787 0.22703794 0.04859170 0.9590084 0.26794583 0.28860000 0.32786667 0.03926667 0.919924846 0.006368319 FALSE TRUE
ENST00000671883 ENSG00000173214 HEK293_OSMI2_6hA HEK293_TMG_6hB MFSD4B protein_coding nonsense_mediated_decay 3.731786 2.363912 4.142418 0.4247419 0.1701059 0.8066855 0.4124919 0.39245507 0.18132352 0.03550400 0.09660883 -1.0728135 0.13315833 0.17836667 0.04363333 -0.13473333 0.299197537 0.006368319 TRUE TRUE
ENST00000672191 ENSG00000173214 HEK293_OSMI2_6hA HEK293_TMG_6hB MFSD4B protein_coding nonsense_mediated_decay 3.731786 2.363912 4.142418 0.4247419 0.1701059 0.8066855 0.3088390 0.03680826 0.00000000 0.01980528 0.00000000 -2.2267632 0.08879167 0.01876667 0.00000000 -0.01876667 0.302069041 0.006368319 TRUE TRUE
ENST00000672303 ENSG00000173214 HEK293_OSMI2_6hA HEK293_TMG_6hB MFSD4B protein_coding protein_coding 3.731786 2.363912 4.142418 0.4247419 0.1701059 0.8066855 0.6434609 0.20821971 1.51621723 0.10075196 0.21996889 2.8061070 0.15573333 0.08820000 0.36643333 0.27823333 0.017946597 0.006368319 FALSE TRUE
ENST00000672494 ENSG00000173214 HEK293_OSMI2_6hA HEK293_TMG_6hB MFSD4B protein_coding processed_transcript 3.731786 2.363912 4.142418 0.4247419 0.1701059 0.8066855 0.3633804 0.25545043 0.14452657 0.05217210 0.07247018 -0.7805876 0.08570833 0.10703333 0.03360000 -0.07343333 0.305881907 0.006368319   FALSE
ENST00000673024 ENSG00000173214 HEK293_OSMI2_6hA HEK293_TMG_6hB MFSD4B protein_coding protein_coding 3.731786 2.363912 4.142418 0.4247419 0.1701059 0.8066855 0.2252368 0.00000000 0.37287792 0.00000000 0.28813412 5.2588126 0.04754583 0.00000000 0.08673333 0.08673333 0.498571352 0.006368319 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000173214 E001 1.1542283 0.017559331 4.402864e-01   6 111259327 111259338 12 + 0.271 0.388 0.734
ENSG00000173214 E002 1.1542283 0.017559331 4.402864e-01   6 111259339 111259340 2 + 0.271 0.388 0.734
ENSG00000173214 E003 6.4311071 0.004470522 5.348461e-01 0.7255363063 6 111259341 111259387 47 + 0.849 0.895 0.177
ENSG00000173214 E004 11.0994056 0.003053167 9.902072e-01 0.9968283736 6 111259388 111259496 109 + 1.088 1.059 -0.105
ENSG00000173214 E005 9.4252709 0.010720558 7.593949e-01 0.8721771201 6 111259497 111259550 54 + 1.011 1.016 0.019
ENSG00000173214 E006 8.5762478 0.061176036 5.663560e-01 0.7475537604 6 111259551 111259612 62 + 0.960 0.989 0.108
ENSG00000173214 E007 6.7742159 0.054082060 6.603375e-01 0.8101684856 6 111259613 111259623 11 + 0.930 0.812 -0.455
ENSG00000173214 E008 13.6507247 0.002921300 2.266917e-01 0.4613912383 6 111259624 111259727 104 + 1.220 1.073 -0.526
ENSG00000173214 E009 24.6335036 0.002011141 1.718135e-02 0.0861060186 6 111262257 111262425 169 + 1.480 1.272 -0.721
ENSG00000173214 E010 21.9546800 0.022904970 1.014340e-01 0.2844161965 6 111263830 111263946 117 + 1.441 1.220 -0.773
ENSG00000173214 E011 0.0000000       6 111265384 111265437 54 +      
ENSG00000173214 E012 117.5311805 0.016801395 3.353698e-04 0.0040929582 6 111265876 111267658 1783 + 2.175 1.896 -0.934
ENSG00000173214 E013 11.9999011 0.002709046 3.235382e-03 0.0248133641 6 111267659 111267837 179 + 1.227 0.895 -1.212
ENSG00000173214 E014 42.4721165 0.001277812 9.974612e-06 0.0002092291 6 111267838 111269154 1317 + 1.736 1.454 -0.961
ENSG00000173214 E015 3.2774645 0.007238296 1.087155e-01 0.2969541177 6 111269155 111269169 15 + 0.727 0.448 -1.263
ENSG00000173214 E016 98.5892374 0.011387857 8.755689e-03 0.0527406564 6 111269170 111273329 4160 + 2.062 1.893 -0.570
ENSG00000173214 E017 7.1586375 0.003817804 1.540731e-01 0.3681240597 6 111273330 111273380 51 + 0.985 0.782 -0.777
ENSG00000173214 E018 5.3798603 0.005555074 6.215244e-01 0.7848590815 6 111273381 111273401 21 + 0.849 0.755 -0.372
ENSG00000173214 E019 6.1057750 0.004907809 1.760569e-01 0.3985502036 6 111273402 111273422 21 + 0.930 0.726 -0.795
ENSG00000173214 E020 4.5599721 0.005868368 2.727638e-01 0.5125938666 6 111273423 111273429 7 + 0.812 0.628 -0.756
ENSG00000173214 E021 43.7278290 0.004675758 4.577958e-01 0.6716168446 6 111273430 111274526 1097 + 1.678 1.604 -0.253
ENSG00000173214 E022 44.2386343 0.003950917 5.007841e-03 0.0346654129 6 111274527 111275561 1035 + 1.582 1.718 0.462
ENSG00000173214 E023 44.3588872 0.004328742 4.651619e-05 0.0007821306 6 111275562 111276716 1155 + 1.534 1.751 0.738
ENSG00000173214 E024 31.3648458 0.010355786 1.866736e-03 0.0161725704 6 111276717 111277207 491 + 1.389 1.606 0.744
ENSG00000173214 E025 13.3922780 0.020316279 6.495179e-02 0.2137946529 6 111277208 111277325 118 + 1.048 1.234 0.667
ENSG00000173214 E026 44.6767026 0.007698643 1.247065e-05 0.0002539638 6 111277326 111277977 652 + 1.519 1.772 0.861
ENSG00000173214 E027 8.7672621 0.003598395 1.794644e-01 0.4032813049 6 111277978 111277999 22 + 0.930 1.045 0.429
ENSG00000173214 E028 15.8339354 0.002833536 6.738229e-05 0.0010744659 6 111278000 111278158 159 + 1.057 1.369 1.106
ENSG00000173214 E029 17.5314060 0.006025333 3.933736e-05 0.0006787632 6 111278159 111278412 254 + 1.088 1.419 1.167
ENSG00000173214 E030 24.5653507 0.001463152 4.773777e-04 0.0054714887 6 111278413 111278773 361 + 1.289 1.501 0.733
ENSG00000173214 E031 17.3182876 0.012275156 5.966610e-05 0.0009689850 6 111278774 111280005 1232 + 1.045 1.423 1.335
ENSG00000173214 E032 1.1962820 0.020209634 1.356173e-01   6 111295450 111295703 254 + 0.198 0.449 1.650
ENSG00000173214 E033 1.3335674 0.013410304 7.739420e-01   6 111295704 111295775 72 + 0.388 0.319 -0.412
ENSG00000173214 E034 0.3503582 0.024643130 8.289627e-01   6 111295776 111295811 36 + 0.110 0.134 0.322
ENSG00000173214 E035 0.5705386 0.962853870 4.490975e-01   6 111326114 111326326 213 + 0.319 0.000 -10.052
ENSG00000173214 E036 0.5421338 0.022403081 7.725846e-01   6 111354615 111355782 1168 + 0.198 0.134 -0.677
ENSG00000173214 E037 0.0000000       6 111355783 111355850 68 +      
ENSG00000173214 E038 0.0000000       6 111404380 111405287 908 +      
ENSG00000173214 E039 0.0000000       6 111440703 111440765 63 +      
ENSG00000173214 E040 0.0000000       6 111444933 111445354 422 +