ENSG00000173141

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000309594 ENSG00000173141 HEK293_OSMI2_6hA HEK293_TMG_6hB MRPL57 protein_coding protein_coding 29.14972 31.22398 18.7319 5.875554 0.7873143 -0.7368494 11.43883 11.732548 8.1564114 2.6213831 0.1806876 -0.52397138 0.4185000 0.3693333 0.43726667 0.06793333 0.60157735 0.03262443 FALSE FALSE
MSTRG.8438.1 ENSG00000173141 HEK293_OSMI2_6hA HEK293_TMG_6hB MRPL57 protein_coding   29.14972 31.22398 18.7319 5.875554 0.7873143 -0.7368494 11.84289 10.496149 10.2441313 2.9823515 0.5889151 -0.03502869 0.4163583 0.3217000 0.54613333 0.22443333 0.03262443 0.03262443 FALSE FALSE
MSTRG.8438.3 ENSG00000173141 HEK293_OSMI2_6hA HEK293_TMG_6hB MRPL57 protein_coding   29.14972 31.22398 18.7319 5.875554 0.7873143 -0.7368494 5.86800 8.995287 0.3313575 0.6388965 0.3313575 -4.72141670 0.1651292 0.3089667 0.01656667 -0.29240000 0.03453958 0.03262443 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000173141 E001 19.31391 0.0021424359 8.415911e-06 1.806618e-04 13 21176644 21176657 14 + 0.902 1.356 1.636
ENSG00000173141 E002 71.78680 0.0109241138 1.703316e-03 1.504067e-02 13 21176658 21176674 17 + 1.650 1.874 0.759
ENSG00000173141 E003 262.76124 0.0003233963 1.435985e-22 5.593195e-20 13 21176675 21176717 43 + 2.198 2.441 0.811
ENSG00000173141 E004 11.88975 0.0086708686 5.261484e-01 7.193227e-01 13 21176862 21176911 50 + 1.020 1.095 0.276
ENSG00000173141 E005 1017.07158 0.0002503308 4.403321e-07 1.385557e-05 13 21176912 21178199 1288 + 2.992 2.956 -0.118
ENSG00000173141 E006 48.13196 0.0012971909 1.537342e-17 3.283974e-15 13 21178200 21178583 384 + 1.915 1.477 -1.487
ENSG00000173141 E007 35.96199 0.0010660337 1.425708e-12 1.425901e-10 13 21178584 21179084 501 + 1.777 1.364 -1.411