ENSG00000171984

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000303142 ENSG00000171984 HEK293_OSMI2_6hA HEK293_TMG_6hB SHLD1 protein_coding protein_coding 8.580918 8.337011 4.593474 1.586571 0.657965 -0.8585367 3.592686 3.627148 2.0191630 0.7736283 0.1616193 -0.8419227 0.4215875 0.4338000 0.45713333 0.02333333 0.97856535 0.03770414 FALSE TRUE
ENST00000378979 ENSG00000171984 HEK293_OSMI2_6hA HEK293_TMG_6hB SHLD1 protein_coding protein_coding 8.580918 8.337011 4.593474 1.586571 0.657965 -0.8585367 3.073638 1.908071 2.1087399 0.5568096 0.4196614 0.1435501 0.3772375 0.2209000 0.45373333 0.23283333 0.03770414 0.03770414   FALSE
MSTRG.20321.3 ENSG00000171984 HEK293_OSMI2_6hA HEK293_TMG_6hB SHLD1 protein_coding   8.580918 8.337011 4.593474 1.586571 0.657965 -0.8585367 1.713154 2.607214 0.3279959 0.4218056 0.3279959 -2.9529541 0.1769583 0.3208333 0.05766667 -0.26316667 0.18450632 0.03770414   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000171984 E001 1.9876007 0.010362577 0.330159695 0.56910415 20 5750393 5750400 8 + 0.554 0.390 -0.828
ENSG00000171984 E002 2.3683188 0.010140072 0.292879544 0.53356137 20 5750401 5750404 4 + 0.603 0.431 -0.828
ENSG00000171984 E003 19.3897223 0.002002189 0.720642626 0.84862939 20 5750405 5750436 32 + 1.299 1.272 -0.097
ENSG00000171984 E004 20.7351059 0.001813439 0.709679734 0.84161688 20 5750437 5750452 16 + 1.327 1.299 -0.097
ENSG00000171984 E005 25.2634176 0.001490004 0.264248914 0.50349999 20 5750453 5750479 27 + 1.442 1.367 -0.259
ENSG00000171984 E006 1.3853673 0.016383139 0.246801966   20 5756712 5756848 137 + 0.498 0.294 -1.150
ENSG00000171984 E007 0.5177432 0.021768165 0.943390561   20 5765094 5765184 91 + 0.155 0.172 0.174
ENSG00000171984 E008 50.2423328 0.001149274 0.667595308 0.81468501 20 5772862 5773043 182 + 1.699 1.679 -0.068
ENSG00000171984 E009 12.3820976 0.018249225 0.218526945 0.45167987 20 5773132 5773447 316 + 1.180 1.052 -0.462
ENSG00000171984 E010 1.8415694 0.013309386 0.980022882 0.99181384 20 5817485 5817547 63 + 0.435 0.431 -0.020
ENSG00000171984 E011 101.9940224 0.001219079 0.009195757 0.05469206 20 5863024 5864395 1372 + 1.933 2.013 0.267