ENSG00000171004

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370836 ENSG00000171004 HEK293_OSMI2_6hA HEK293_TMG_6hB HS6ST2 protein_coding protein_coding 17.59124 6.554483 24.05996 1.253973 2.803169 1.87448 8.9838637 2.493106 11.0120541 0.67753432 0.2047067 2.138602 0.4486375 0.3756667 0.46993333 0.09426667 0.7441509772 0.0001418726 FALSE TRUE
ENST00000406696 ENSG00000171004 HEK293_OSMI2_6hA HEK293_TMG_6hB HS6ST2 protein_coding protein_coding 17.59124 6.554483 24.05996 1.253973 2.803169 1.87448 6.9998785 2.723853 10.9313811 0.74357465 3.4706034 2.000787 0.3952375 0.4030000 0.42956667 0.02656667 0.9897017069 0.0001418726 FALSE TRUE
ENST00000640529 ENSG00000171004 HEK293_OSMI2_6hA HEK293_TMG_6hB HS6ST2 protein_coding protein_coding 17.59124 6.554483 24.05996 1.253973 2.803169 1.87448 0.1693244 0.000000 1.0245725 0.00000000 0.7008320 6.692891 0.0077750 0.0000000 0.05160000 0.05160000 0.5782748142 0.0001418726 FALSE TRUE
MSTRG.34892.11 ENSG00000171004 HEK293_OSMI2_6hA HEK293_TMG_6hB HS6ST2 protein_coding   17.59124 6.554483 24.05996 1.253973 2.803169 1.87448 0.7767541 1.038202 0.4696187 0.04236805 0.0708143 -1.127957 0.0910750 0.1769667 0.02063333 -0.15633333 0.0001418726 0.0001418726 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000171004 E001 0.2852693 0.4393191403 6.181764e-01   X 132626015 132626015 1 - 0.144 0.000 -10.640
ENSG00000171004 E002 0.2852693 0.4393191403 6.181764e-01   X 132626016 132626027 12 - 0.144 0.000 -10.631
ENSG00000171004 E003 603.8607567 0.8951289832 4.162800e-01 6.408298e-01 X 132626028 132627969 1942 - 2.732 2.752 0.068
ENSG00000171004 E004 286.6437440 0.3074544591 7.242835e-01 8.507151e-01 X 132627970 132628743 774 - 2.452 2.306 -0.489
ENSG00000171004 E005 96.8106792 0.0016227030 8.545258e-08 3.226281e-06 X 132628744 132628904 161 - 2.025 1.764 -0.880
ENSG00000171004 E006 38.4788400 0.0213308060 4.749545e-02 1.737537e-01 X 132628905 132628911 7 - 1.617 1.410 -0.711
ENSG00000171004 E007 80.7599534 0.0009233532 4.103945e-04 4.831090e-03 X 132628912 132629093 182 - 1.934 1.757 -0.597
ENSG00000171004 E008 1.9574986 0.1229854189 4.556015e-01 6.699668e-01 X 132669113 132669199 87 - 0.412 0.552 0.694
ENSG00000171004 E009 2.9197004 0.0302340477 5.857855e-01 7.607994e-01 X 132708462 132708494 33 - 0.532 0.615 0.374
ENSG00000171004 E010 0.3088520 1.5113450649 1.000000e+00   X 132709923 132709937 15 - 0.083 0.171 1.199
ENSG00000171004 E011 8.3840195 0.7470042530 1.385058e-01 3.452440e-01 X 132709938 132710140 203 - 0.481 1.350 3.400
ENSG00000171004 E012 0.3032425 0.3745319016 1.532529e-01   X 132735084 132735393 310 - 0.000 0.305 13.321
ENSG00000171004 E013 8.2964952 0.6582298525 7.700959e-02 2.388266e-01 X 132758256 132758332 77 - 0.419 1.364 3.769
ENSG00000171004 E014 65.0057336 0.0008131471 4.188259e-03 3.020352e-02 X 132956808 132956915 108 - 1.757 1.873 0.394
ENSG00000171004 E015 67.5079454 0.0006719795 3.960627e-03 2.892803e-02 X 132956916 132957016 101 - 1.773 1.888 0.386
ENSG00000171004 E016 113.5971536 0.0013034822 4.897896e-07 1.520358e-05 X 132957017 132957326 310 - 1.975 2.146 0.573
ENSG00000171004 E017 0.0000000       X 132957446 132957515 70 -      
ENSG00000171004 E018 0.5008152 1.1073270760 3.644187e-01   X 132957682 132957784 103 - 0.210 0.000 -11.289
ENSG00000171004 E019 1.4155550 0.9882336184 2.560604e-01 4.947938e-01 X 132957874 132957920 47 - 0.419 0.173 -1.727
ENSG00000171004 E020 2.2016395 0.9000197375 1.266430e-01 3.264903e-01 X 132957921 132958174 254 - 0.559 0.174 -2.415
ENSG00000171004 E021 58.5481684 0.7336478953 6.351520e-01 7.936675e-01 X 132958175 132958868 694 - 1.702 1.798 0.325
ENSG00000171004 E022 0.8428035 2.6166861419 8.795794e-01   X 132958869 132959638 770 - 0.274 0.167 -0.911
ENSG00000171004 E023 0.1308682 0.8400332404 4.737651e-01   X 132961246 132961395 150 - 0.000 0.174 12.274