ENSG00000170525

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000379789 ENSG00000170525 HEK293_OSMI2_6hA HEK293_TMG_6hB PFKFB3 protein_coding protein_coding 23.67595 30.45001 18.46654 1.619856 1.050014 -0.7212214 13.681380 16.745663 13.170157 0.270100 1.2289489 -0.3462813 0.5926958 0.5526667 0.7101667 0.1575000 8.682194e-02 3.660678e-19 FALSE TRUE
ENST00000441697 ENSG00000170525 HEK293_OSMI2_6hA HEK293_TMG_6hB PFKFB3 protein_coding protein_coding 23.67595 30.45001 18.46654 1.619856 1.050014 -0.7212214 3.671489 4.513637 0.000000 0.760586 0.0000000 -8.8213394 0.1348708 0.1467333 0.0000000 -0.1467333 3.660678e-19 3.660678e-19 FALSE TRUE
MSTRG.3496.1 ENSG00000170525 HEK293_OSMI2_6hA HEK293_TMG_6hB PFKFB3 protein_coding   23.67595 30.45001 18.46654 1.619856 1.050014 -0.7212214 2.644705 3.540517 2.024898 0.777574 0.1334463 -0.8030730 0.1133625 0.1143333 0.1110333 -0.0033000 1.000000e+00 3.660678e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000170525 E001 12.5533114 0.0034768047 0.077063441 0.238930704 10 6144918 6144933 16 + 0.963 1.163 0.726
ENSG00000170525 E002 63.6238526 0.0066098933 0.764624668 0.875391971 10 6144934 6145013 80 + 1.770 1.801 0.103
ENSG00000170525 E003 0.1614157 0.0329592374 0.266917105   10 6146316 6146496 181 + 0.156 0.000 -11.258
ENSG00000170525 E004 6.7408484 0.0104636066 0.539662772 0.728752224 10 6163833 6164011 179 + 0.820 0.914 0.364
ENSG00000170525 E005 0.1779838 0.0348669859 0.266706033   10 6202866 6202876 11 + 0.156 0.000 -11.232
ENSG00000170525 E006 0.3206185 0.0274424043 0.081099602   10 6202877 6202899 23 + 0.270 0.000 -12.341
ENSG00000170525 E007 0.6709767 0.0367313657 0.112150730   10 6202900 6202931 32 + 0.361 0.094 -2.422
ENSG00000170525 E008 27.7942532 0.0012322937 0.267023088 0.506628966 10 6202932 6203336 405 + 1.378 1.470 0.318
ENSG00000170525 E009 138.7641603 0.0004052552 0.078788104 0.242391889 10 6213623 6213748 126 + 2.151 2.110 -0.137
ENSG00000170525 E010 139.5987584 0.0003218242 0.110273995 0.299436540 10 6215221 6215317 97 + 2.150 2.115 -0.117
ENSG00000170525 E011 135.0554600 0.0003278201 0.067490188 0.219421417 10 6216125 6216191 67 + 2.139 2.096 -0.144
ENSG00000170525 E012 138.5439961 0.0003101097 0.004503409 0.031956477 10 6216706 6216780 75 + 2.169 2.096 -0.245
ENSG00000170525 E013 120.0157134 0.0003475651 0.004187902 0.030202232 10 6217135 6217191 57 + 2.112 2.031 -0.270
ENSG00000170525 E014 175.6620993 0.0002913678 0.003612048 0.026997442 10 6219569 6219693 125 + 2.268 2.203 -0.219
ENSG00000170525 E015 205.2592741 0.0002368944 0.346080816 0.583635934 10 6220658 6220865 208 + 2.299 2.290 -0.032
ENSG00000170525 E016 81.4161169 0.0005861346 0.924911434 0.964784468 10 6221381 6221403 23 + 1.881 1.899 0.062
ENSG00000170525 E017 144.5361227 0.0003254393 0.801733629 0.897347952 10 6221404 6221527 124 + 2.125 2.147 0.075
ENSG00000170525 E018 124.6976379 0.0009448382 0.176743326 0.399423547 10 6221641 6221745 105 + 2.038 2.102 0.214
ENSG00000170525 E019 3.5445794 0.0980241994 0.944206934 0.974456300 10 6222708 6222854 147 + 0.604 0.649 0.193
ENSG00000170525 E020 137.8639799 0.0003696181 0.718911080 0.847522702 10 6222855 6222984 130 + 2.121 2.124 0.011
ENSG00000170525 E021 121.3292893 0.0010439482 0.343518419 0.581182228 10 6223958 6224020 63 + 2.079 2.061 -0.061
ENSG00000170525 E022 88.0389704 0.0030109032 0.425132016 0.647354169 10 6224149 6224165 17 + 1.943 1.923 -0.067
ENSG00000170525 E023 97.9438775 0.0035688952 0.642318788 0.798393439 10 6224166 6224213 48 + 1.980 1.976 -0.013
ENSG00000170525 E024 1.1969022 0.0287365843 0.691581410   10 6224506 6224623 118 + 0.360 0.295 -0.411
ENSG00000170525 E025 1.6911278 0.6673454624 0.301954792 0.542505492 10 6225161 6225248 88 + 0.564 0.301 -1.413
ENSG00000170525 E026 153.1914490 0.0007140498 0.657162577 0.808107922 10 6226192 6226365 174 + 2.151 2.180 0.098
ENSG00000170525 E027 2.8632642 0.0089057219 0.994232874 0.998684592 10 6226366 6226447 82 + 0.555 0.564 0.042
ENSG00000170525 E028 3.2798968 0.1009213406 0.823946120 0.910254577 10 6228196 6228218 23 + 0.645 0.615 -0.132
ENSG00000170525 E029 2.9157469 0.2545804860 0.867635468 0.934630244 10 6228219 6228295 77 + 0.598 0.584 -0.062
ENSG00000170525 E030 3.2244815 0.0262748937 0.060049046 0.202947562 10 6229109 6229180 72 + 0.761 0.468 -1.296
ENSG00000170525 E031 2.6419862 0.0145373781 0.403693437 0.631308694 10 6231263 6231314 52 + 0.436 0.591 0.747
ENSG00000170525 E032 1547.8865159 0.0014130617 0.001010097 0.009988795 10 6232895 6235532 2638 + 3.128 3.191 0.210
ENSG00000170525 E033 2.4826188 0.0090229307 0.370283515 0.604193600 10 6254178 6254644 467 + 0.604 0.467 -0.642