ENSG00000170473

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000398213 ENSG00000170473 HEK293_OSMI2_6hA HEK293_TMG_6hB PYM1 protein_coding protein_coding 38.02602 44.69289 24.33735 7.022807 0.7893422 -0.8766034 11.272204 8.105825 10.806645 1.0233938 0.31538045 0.4144434 0.32155417 0.1858333 0.44436667 0.25853333 7.263168e-07 7.263168e-07 FALSE TRUE
ENST00000408946 ENSG00000170473 HEK293_OSMI2_6hA HEK293_TMG_6hB PYM1 protein_coding protein_coding 38.02602 44.69289 24.33735 7.022807 0.7893422 -0.8766034 21.382573 31.760924 9.945172 5.7704508 0.96937189 -1.6741888 0.53170000 0.7045000 0.40696667 -0.29753333 1.167669e-05 7.263168e-07 FALSE TRUE
ENST00000549939 ENSG00000170473 HEK293_OSMI2_6hA HEK293_TMG_6hB PYM1 protein_coding protein_coding 38.02602 44.69289 24.33735 7.022807 0.7893422 -0.8766034 2.399429 1.797233 1.665983 0.1897488 0.06840665 -0.1087747 0.06449583 0.0413000 0.06843333 0.02713333 1.712011e-01 7.263168e-07   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000170473 E001 0.5474829 0.1512350630 3.125116e-01   12 55901413 55901413 1 - 0.000 0.219 9.642
ENSG00000170473 E002 67.1284878 0.0007024058 3.739231e-01 6.072947e-01 12 55901414 55901500 87 - 1.822 1.781 -0.140
ENSG00000170473 E003 224.7129276 0.0013039894 6.279924e-01 7.892290e-01 12 55901501 55901686 186 - 2.326 2.310 -0.052
ENSG00000170473 E004 371.5614794 0.0003231935 1.301811e-01 3.320978e-01 12 55901687 55901970 284 - 2.514 2.541 0.092
ENSG00000170473 E005 198.8477309 0.0002418017 6.667777e-02 2.175991e-01 12 55901971 55902040 70 - 2.229 2.277 0.160
ENSG00000170473 E006 398.9953772 0.0002055455 8.133524e-01 9.042615e-01 12 55902041 55902355 315 - 2.563 2.566 0.012
ENSG00000170473 E007 186.1724497 0.0003862249 4.907007e-01 6.944818e-01 12 55903387 55903480 94 - 2.251 2.232 -0.064
ENSG00000170473 E008 1.5489959 0.0132317344 6.247350e-02 2.083025e-01 12 55913746 55913825 80 - 0.591 0.269 -1.756
ENSG00000170473 E009 16.8312587 0.0021020342 1.190956e-01 3.143900e-01 12 55913826 55914018 193 - 1.309 1.175 -0.473
ENSG00000170473 E010 36.0764743 0.0012156162 8.630076e-03 5.218763e-02 12 55914019 55914275 257 - 1.640 1.480 -0.545
ENSG00000170473 E011 28.7759215 0.0033695393 9.413410e-02 2.714253e-01 12 55914276 55914366 91 - 1.519 1.399 -0.413
ENSG00000170473 E012 3.4214186 0.0165474874 2.069119e-01 4.380637e-01 12 55915443 55915531 89 - 0.730 0.528 -0.880
ENSG00000170473 E013 56.5301346 0.0038318216 4.769151e-08 1.910619e-06 12 55927076 55927267 192 - 1.911 1.615 -0.999
ENSG00000170473 E014 2.7735944 0.0237832349 2.017923e-01 4.318602e-01 12 55927318 55927724 407 - 0.390 0.623 1.137
ENSG00000170473 E015 107.5895938 0.0016379042 1.118268e-06 3.128112e-05 12 55927725 55927894 170 - 1.847 2.055 0.700
ENSG00000170473 E016 0.0000000       12 55932529 55932618 90 -