ENSG00000169925

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000303407 ENSG00000169925 HEK293_OSMI2_6hA HEK293_TMG_6hB BRD3 protein_coding protein_coding 18.77506 21.82457 18.27268 2.229587 0.200357 -0.2561362 14.472260 17.669111 13.576437 1.5012075 0.6110575 -0.3798785 0.7727333 0.8131667 0.74253333 -0.07063333 5.574806e-01 2.280989e-12 FALSE TRUE
ENST00000494743 ENSG00000169925 HEK293_OSMI2_6hA HEK293_TMG_6hB BRD3 protein_coding retained_intron 18.77506 21.82457 18.27268 2.229587 0.200357 -0.2561362 1.902855 2.271341 1.600464 0.7302826 0.6782587 -0.5024057 0.1016958 0.0988000 0.08823333 -0.01056667 9.508566e-01 2.280989e-12 FALSE FALSE
MSTRG.33581.4 ENSG00000169925 HEK293_OSMI2_6hA HEK293_TMG_6hB BRD3 protein_coding   18.77506 21.82457 18.27268 2.229587 0.200357 -0.2561362 1.062531 0.000000 1.546333 0.0000000 0.3016515 7.2820067 0.0544250 0.0000000 0.08500000 0.08500000 2.280989e-12 2.280989e-12 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000169925 E001 827.4736382 0.0796367993 3.383432e-02 1.384174e-01 9 134030305 134032013 1709 - 2.740 3.012 0.903
ENSG00000169925 E002 171.1265910 0.0050806169 3.383038e-04 4.122839e-03 9 134032014 134032165 152 - 2.091 2.314 0.746
ENSG00000169925 E003 264.1633974 0.0101121476 1.804707e-02 8.919437e-02 9 134032166 134032929 764 - 2.303 2.490 0.624
ENSG00000169925 E004 30.5443798 0.0145930928 9.523072e-01 9.781349e-01 9 134032930 134032944 15 - 1.463 1.532 0.236
ENSG00000169925 E005 63.2062311 0.0005755880 2.373560e-01 4.734936e-01 9 134032945 134032992 48 - 1.800 1.807 0.023
ENSG00000169925 E006 351.0490355 0.0017304042 1.497032e-03 1.359584e-02 9 134032993 134033401 409 - 2.553 2.532 -0.068
ENSG00000169925 E007 154.7303876 0.0003484211 1.979687e-03 1.693258e-02 9 134033402 134033488 87 - 2.204 2.175 -0.098
ENSG00000169925 E008 175.4854539 0.0002852026 8.628133e-01 9.319184e-01 9 134033489 134033705 217 - 2.204 2.269 0.218
ENSG00000169925 E009 137.3606321 0.0009764989 1.303140e-01 3.323200e-01 9 134034701 134034826 126 - 2.129 2.139 0.035
ENSG00000169925 E010 66.9663901 0.0015369754 7.517403e-03 4.710978e-02 9 134034827 134034829 3 - 1.861 1.797 -0.218
ENSG00000169925 E011 0.6653672 0.1330588094 7.150106e-01   9 134036028 134036031 4 - 0.238 0.197 -0.352
ENSG00000169925 E012 241.0453948 0.0003140816 5.647082e-04 6.262272e-03 9 134036032 134036320 289 - 2.392 2.371 -0.067
ENSG00000169925 E013 64.6589722 0.0005576198 8.985468e-02 2.635246e-01 9 134036321 134036324 4 - 1.823 1.807 -0.051
ENSG00000169925 E014 5.6112164 0.1065796097 3.605111e-01 5.960770e-01 9 134036325 134036591 267 - 0.876 0.770 -0.414
ENSG00000169925 E015 164.5454915 0.0043379134 1.216963e-01 3.185541e-01 9 134040034 134040269 236 - 2.217 2.216 -0.001
ENSG00000169925 E016 161.2239955 0.0004145611 7.520287e-04 7.878093e-03 9 134041760 134041951 192 - 2.226 2.190 -0.121
ENSG00000169925 E017 161.6724200 0.0002879676 1.438720e-06 3.902998e-05 9 134045293 134045421 129 - 2.249 2.175 -0.249
ENSG00000169925 E018 9.4103604 0.0584699598 1.907117e-01 4.178027e-01 9 134048002 134048082 81 - 0.836 1.116 1.042
ENSG00000169925 E019 261.2779949 0.0026505063 1.004563e-04 1.500949e-03 9 134048083 134048454 372 - 2.448 2.388 -0.199
ENSG00000169925 E020 3.3218355 0.0083998577 8.283565e-01 9.128509e-01 9 134049676 134049757 82 - 0.594 0.672 0.340
ENSG00000169925 E021 135.6822846 0.0068171791 1.856208e-02 9.094042e-02 9 134050374 134050549 176 - 2.161 2.109 -0.173
ENSG00000169925 E022 42.6365057 0.0107779029 5.413316e-01 7.300246e-01 9 134050550 134050588 39 - 1.627 1.645 0.060
ENSG00000169925 E023 96.1931025 0.0010346617 7.321025e-01 8.554864e-01 9 134051562 134051709 148 - 1.959 2.007 0.161
ENSG00000169925 E024 88.1818291 0.0004907797 1.191860e-02 6.610231e-02 9 134052306 134052432 127 - 1.967 1.932 -0.119
ENSG00000169925 E025 44.8204425 0.0017053463 9.302507e-04 9.340071e-03 9 134052433 134052443 11 - 1.721 1.599 -0.412
ENSG00000169925 E026 87.6014635 0.0021214839 2.724836e-03 2.171061e-02 9 134053265 134053417 153 - 1.978 1.910 -0.226
ENSG00000169925 E027 65.5053374 0.0080820332 3.205856e-01 5.602185e-01 9 134053418 134053590 173 - 1.817 1.820 0.012
ENSG00000169925 E028 4.6314717 0.0059519034 2.201309e-03 1.841269e-02 9 134053715 134054140 426 - 0.391 0.909 2.281
ENSG00000169925 E029 1.1707238 0.4593299690 3.895821e-01   9 134055776 134056012 237 - 0.137 0.436 2.219
ENSG00000169925 E030 22.9990551 0.0014662385 5.429270e-01 7.311660e-01 9 134067945 134068077 133 - 1.317 1.420 0.358
ENSG00000169925 E031 1.9283586 0.0390460121 5.052031e-01 7.044465e-01 9 134068327 134068535 209 - 0.505 0.431 -0.374