ENSG00000169251

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000351193 ENSG00000169251 HEK293_OSMI2_6hA HEK293_TMG_6hB NMD3 protein_coding protein_coding 26.65973 3.915649 54.90827 0.7353963 4.448821 3.806283 15.461638 2.154215 34.15681 0.3593094 2.067430 3.9806800 0.6204792 0.5600 0.62436667 0.06436667 7.169891e-01 1.091947e-09 FALSE TRUE
ENST00000473909 ENSG00000169251 HEK293_OSMI2_6hA HEK293_TMG_6hB NMD3 protein_coding retained_intron 26.65973 3.915649 54.90827 0.7353963 4.448821 3.806283 5.205856 0.000000 13.80742 0.0000000 3.326756 10.4322724 0.1053083 0.0000 0.24530000 0.24530000 1.091947e-09 1.091947e-09 FALSE TRUE
MSTRG.24022.3 ENSG00000169251 HEK293_OSMI2_6hA HEK293_TMG_6hB NMD3 protein_coding   26.65973 3.915649 54.90827 0.7353963 4.448821 3.806283 1.999701 1.293270 1.46201 0.2996086 1.462010 0.1756513 0.1372667 0.3357 0.03173333 -0.30396667 8.488306e-02 1.091947e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000169251 E001 0.0000000       3 161104696 161104821 126 +      
ENSG00000169251 E002 0.0000000       3 161104822 161104881 60 +      
ENSG00000169251 E003 0.0000000       3 161104882 161104885 4 +      
ENSG00000169251 E004 0.0000000       3 161156971 161157055 85 +      
ENSG00000169251 E005 0.1779838 0.0369108818 6.066171e-01   3 161221254 161221261 8 + 0.042 0.000 -8.624
ENSG00000169251 E006 0.1779838 0.0369108818 6.066171e-01   3 161221262 161221275 14 + 0.042 0.000 -8.607
ENSG00000169251 E007 1.2140381 0.0223604600 3.870893e-02   3 161221276 161221287 12 + 0.180 0.606 2.566
ENSG00000169251 E008 6.3266637 0.0051872765 9.393791e-01 0.9719268036 3 161221288 161221307 20 + 0.671 0.704 0.138
ENSG00000169251 E009 12.7836277 0.0027041719 4.047312e-01 0.6320902724 3 161221308 161221310 3 + 0.912 1.048 0.502
ENSG00000169251 E010 33.3923416 0.0011307496 2.122215e-01 0.4443578346 3 161221311 161221323 13 + 1.322 1.211 -0.390
ENSG00000169251 E011 47.1761786 0.0010982521 3.791004e-01 0.6114828047 3 161221324 161221331 8 + 1.460 1.405 -0.192
ENSG00000169251 E012 60.2133945 0.0007387982 9.685595e-02 0.2762156999 3 161221332 161221409 78 + 1.566 1.454 -0.386
ENSG00000169251 E013 5.7762614 0.0218580537 7.959595e-01 0.8939843948 3 161221410 161221556 147 + 0.643 0.607 -0.157
ENSG00000169251 E014 2.2086540 0.0096388007 5.825372e-01 0.7587267856 3 161221557 161221558 2 + 0.348 0.481 0.723
ENSG00000169251 E015 7.4027695 0.0037866161 1.699414e-01 0.3903840280 3 161221559 161221724 166 + 0.749 0.482 -1.181
ENSG00000169251 E016 0.1779838 0.0369108818 6.066171e-01   3 161221725 161221735 11 + 0.042 0.000 -8.607
ENSG00000169251 E017 0.5008152 0.0409410833 1.000000e+00   3 161221736 161221993 258 + 0.117 0.000 -10.193
ENSG00000169251 E018 110.5268047 0.0004215174 1.715619e-02 0.0860230787 3 161221994 161222057 64 + 1.828 1.706 -0.412
ENSG00000169251 E019 146.6771628 0.0007589745 1.373533e-02 0.0733006908 3 161224930 161225018 89 + 1.948 1.839 -0.366
ENSG00000169251 E020 123.4274926 0.0035421037 1.592425e-02 0.0815089418 3 161225019 161225064 46 + 1.875 1.740 -0.457
ENSG00000169251 E021 160.1388432 0.0012008060 4.051002e-04 0.0047791132 3 161227247 161227343 97 + 1.989 1.820 -0.570
ENSG00000169251 E022 139.4496233 0.0039248475 5.295474e-05 0.0008742037 3 161233399 161233479 81 + 1.934 1.688 -0.830
ENSG00000169251 E023 170.0582720 0.0045909644 1.885762e-01 0.4151555075 3 161234727 161234855 129 + 2.006 1.951 -0.184
ENSG00000169251 E024 1.4461025 0.0120459090 4.182928e-01 0.6423491480 3 161234856 161234905 50 + 0.284 0.000 -11.776
ENSG00000169251 E025 125.5501444 0.0004385925 5.825661e-01 0.7587477821 3 161235122 161235212 91 + 1.866 1.915 0.166
ENSG00000169251 E026 0.8380019 0.0299796912 1.000000e+00   3 161235213 161235331 119 + 0.180 0.000 -10.931
ENSG00000169251 E027 143.3594114 0.0010946046 4.921195e-01 0.6954292358 3 161238113 161238191 79 + 1.921 1.976 0.184
ENSG00000169251 E028 176.6993508 0.0033985961 9.744440e-01 0.9890860591 3 161238730 161238826 97 + 2.015 2.036 0.072
ENSG00000169251 E029 182.0423035 0.0017149295 8.293019e-01 0.9133949676 3 161241046 161241163 118 + 2.028 2.059 0.103
ENSG00000169251 E030 178.1939782 0.0002616097 5.702841e-01 0.7504678427 3 161242508 161242653 146 + 2.018 2.064 0.154
ENSG00000169251 E031 144.3643563 0.0004285481 1.656201e-01 0.3846819297 3 161246336 161246448 113 + 1.924 2.011 0.290
ENSG00000169251 E032 134.4244315 0.0003979392 5.954443e-01 0.7673403874 3 161247258 161247330 73 + 1.898 1.946 0.163
ENSG00000169251 E033 185.7009685 0.0002695755 7.507175e-01 0.8671902362 3 161249454 161249560 107 + 2.040 2.048 0.029
ENSG00000169251 E034 169.0794418 0.0002701450 5.235531e-01 0.7174945383 3 161250256 161250326 71 + 1.995 2.044 0.166
ENSG00000169251 E035 220.4964635 0.0002357358 2.001029e-01 0.4298186762 3 161250780 161250890 111 + 2.107 2.177 0.234
ENSG00000169251 E036 595.8262530 0.0138297397 8.817515e-03 0.0530059056 3 161250891 161252307 1417 + 2.524 2.677 0.508
ENSG00000169251 E037 6.9930606 0.0047365024 1.196094e-02 0.0662894874 3 161252769 161253532 764 + 0.652 1.047 1.542