ENSG00000169220

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000502731 ENSG00000169220 HEK293_OSMI2_6hA HEK293_TMG_6hB RGS14 protein_coding retained_intron 21.55477 29.38758 10.91747 3.40324 0.6015995 -1.427738 1.0000651 0.9345998 1.2447663 0.46904703 0.37146650 0.4096436 0.05015000 0.035933333 0.11123333 0.07530000 0.65956953 0.02018202 FALSE  
ENST00000503044 ENSG00000169220 HEK293_OSMI2_6hA HEK293_TMG_6hB RGS14 protein_coding retained_intron 21.55477 29.38758 10.91747 3.40324 0.6015995 -1.427738 2.3778196 3.4558836 0.9286355 0.82369783 0.59769129 -1.8845863 0.10420833 0.114600000 0.07960000 -0.03500000 0.81153643 0.02018202    
ENST00000506944 ENSG00000169220 HEK293_OSMI2_6hA HEK293_TMG_6hB RGS14 protein_coding processed_transcript 21.55477 29.38758 10.91747 3.40324 0.6015995 -1.427738 3.9330058 5.9033555 1.7839681 0.68993611 0.18749267 -1.7208228 0.16877917 0.200833333 0.16610000 -0.03473333 0.75958641 0.02018202 FALSE  
ENST00000509289 ENSG00000169220 HEK293_OSMI2_6hA HEK293_TMG_6hB RGS14 protein_coding retained_intron 21.55477 29.38758 10.91747 3.40324 0.6015995 -1.427738 2.6311643 3.1979618 1.0252032 0.69244444 0.05661875 -1.6317430 0.12700000 0.106666667 0.09463333 -0.01203333 0.90140555 0.02018202 FALSE  
ENST00000512490 ENSG00000169220 HEK293_OSMI2_6hA HEK293_TMG_6hB RGS14 protein_coding retained_intron 21.55477 29.38758 10.91747 3.40324 0.6015995 -1.427738 2.9529976 5.0010327 0.4496024 0.29074821 0.44960243 -3.4466496 0.12854167 0.172500000 0.03743333 -0.13506667 0.21599770 0.02018202 FALSE  
ENST00000514102 ENSG00000169220 HEK293_OSMI2_6hA HEK293_TMG_6hB RGS14 protein_coding retained_intron 21.55477 29.38758 10.91747 3.40324 0.6015995 -1.427738 2.3867889 3.1327335 1.1753275 0.64498877 0.36298532 -1.4067342 0.11306250 0.105600000 0.10610000 0.00050000 1.00000000 0.02018202 FALSE  
ENST00000514713 ENSG00000169220 HEK293_OSMI2_6hA HEK293_TMG_6hB RGS14 protein_coding retained_intron 21.55477 29.38758 10.91747 3.40324 0.6015995 -1.427738 0.4835284 0.1359251 0.6504102 0.09449816 0.16628255 2.1781340 0.02947917 0.004933333 0.06146667 0.05653333 0.02018202 0.02018202 FALSE  
MSTRG.27381.10 ENSG00000169220 HEK293_OSMI2_6hA HEK293_TMG_6hB RGS14 protein_coding   21.55477 29.38758 10.91747 3.40324 0.6015995 -1.427738 3.4012488 5.2155737 1.6392446 0.71670862 0.82046830 -1.6637840 0.15826250 0.177400000 0.15816667 -0.01923333 0.92451918 0.02018202 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000169220 E001 0.0000000       5 177357924 177358069 146 +      
ENSG00000169220 E002 0.0000000       5 177365963 177365984 22 +      
ENSG00000169220 E003 0.0000000       5 177366177 177366355 179 +      
ENSG00000169220 E004 0.0000000       5 177366428 177366565 138 +      
ENSG00000169220 E005 0.0000000       5 177366566 177366707 142 +      
ENSG00000169220 E006 1.5600566 0.0621802819 3.457937e-01 5.833587e-01 5 177366708 177366800 93 + 0.198 0.390 1.334
ENSG00000169220 E007 1.7026913 0.1035519131 7.489556e-01 8.661153e-01 5 177366891 177366942 52 + 0.332 0.392 0.350
ENSG00000169220 E008 2.2379304 0.0445893487 8.321444e-01 9.150894e-01 5 177366943 177367034 92 + 0.436 0.474 0.196
ENSG00000169220 E009 0.1779838 0.0333978612 1.418533e-01   5 177367217 177367265 49 + 0.198 0.000 -12.682
ENSG00000169220 E010 0.0000000       5 177367302 177367302 1 +      
ENSG00000169220 E011 0.1308682 0.0326491905 1.000000e+00   5 177367303 177367313 11 + 0.000 0.072 9.813
ENSG00000169220 E012 2.5774498 0.0208023723 5.833595e-01 7.592396e-01 5 177367314 177367333 20 + 0.437 0.544 0.529
ENSG00000169220 E013 27.1812354 0.0014547399 2.088394e-01 4.404658e-01 5 177367334 177367413 80 + 1.303 1.406 0.356
ENSG00000169220 E014 45.5743276 0.0009510167 9.429218e-02 2.717070e-01 5 177367414 177367557 144 + 1.516 1.623 0.364
ENSG00000169220 E015 6.3752911 0.0250860666 3.432077e-02 1.397376e-01 5 177367558 177367713 156 + 1.012 0.711 -1.166
ENSG00000169220 E016 36.2775568 0.0012183882 4.451600e-01 6.621971e-01 5 177367714 177367825 112 + 1.459 1.514 0.187
ENSG00000169220 E017 18.6249606 0.0141192923 1.352153e-02 7.244889e-02 5 177367826 177368156 331 + 1.395 1.151 -0.857
ENSG00000169220 E018 56.6918833 0.0009621983 8.334740e-01 9.157027e-01 5 177368157 177368266 110 + 1.680 1.693 0.045
ENSG00000169220 E019 86.3218324 0.0006991376 2.009257e-02 9.619903e-02 5 177368717 177368920 204 + 1.943 1.848 -0.320
ENSG00000169220 E020 4.6408418 0.0239723964 2.987902e-06 7.408591e-05 5 177368921 177369075 155 + 1.099 0.419 -2.832
ENSG00000169220 E021 3.2463988 0.1101105990 9.985132e-02 2.814887e-01 5 177370553 177370587 35 + 0.798 0.452 -1.527
ENSG00000169220 E022 26.5175092 0.0016863292 6.842483e-01 8.254548e-01 5 177370588 177370590 3 + 1.393 1.364 -0.102
ENSG00000169220 E023 51.3632795 0.0011157266 7.861721e-01 8.882080e-01 5 177370591 177370664 74 + 1.659 1.645 -0.047
ENSG00000169220 E024 7.5987607 0.0039606609 4.295983e-01 6.507218e-01 5 177370821 177370904 84 + 0.793 0.903 0.428
ENSG00000169220 E025 69.1687684 0.0014989605 7.073716e-01 8.401447e-01 5 177370905 177371031 127 + 1.761 1.781 0.068
ENSG00000169220 E026 0.5649788 0.0219845478 3.168968e-01   5 177371066 177371106 41 + 0.000 0.235 11.834
ENSG00000169220 E027 1.5354261 0.0127878958 4.165615e-02 1.590282e-01 5 177371107 177371112 6 + 0.000 0.446 13.135
ENSG00000169220 E028 12.5131509 0.0025681065 3.577663e-02 1.436472e-01 5 177371113 177371164 52 + 0.867 1.121 0.939
ENSG00000169220 E029 78.5552107 0.0005802273 6.388840e-01 7.962122e-01 5 177371165 177371246 82 + 1.818 1.842 0.082
ENSG00000169220 E030 68.6153764 0.0080116144 7.617185e-01 8.736581e-01 5 177371350 177371396 47 + 1.790 1.776 -0.047
ENSG00000169220 E031 17.5963065 0.0040351866 4.316232e-01 6.524562e-01 5 177371397 177371474 78 + 1.264 1.195 -0.243
ENSG00000169220 E032 87.0304144 0.0052760953 7.809056e-01 8.852496e-01 5 177371475 177371589 115 + 1.888 1.878 -0.036
ENSG00000169220 E033 261.5409329 0.0021765794 2.057886e-01 4.367955e-01 5 177371873 177372596 724 + 2.323 2.359 0.121