ENSG00000169155

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373464 ENSG00000169155 HEK293_OSMI2_6hA HEK293_TMG_6hB ZBTB43 protein_coding protein_coding 5.494604 3.65664 7.001392 0.6967604 0.2887198 0.9352423 3.38113933 0.9292482 5.2701342 0.5057944 0.4916719 2.4909962 0.58209167 0.30006667 0.75086667 0.45080000 4.911977e-01 5.512263e-14 FALSE TRUE
ENST00000449886 ENSG00000169155 HEK293_OSMI2_6hA HEK293_TMG_6hB ZBTB43 protein_coding protein_coding 5.494604 3.65664 7.001392 0.6967604 0.2887198 0.9352423 0.81056803 1.1661726 1.3069554 0.4912120 0.6886698 0.1631066 0.14106250 0.28823333 0.19236667 -0.09586667 8.696152e-01 5.512263e-14 FALSE TRUE
ENST00000450858 ENSG00000169155 HEK293_OSMI2_6hA HEK293_TMG_6hB ZBTB43 protein_coding protein_coding 5.494604 3.65664 7.001392 0.6967604 0.2887198 0.9352423 0.07899112 0.0000000 0.4090881 0.0000000 0.4090881 5.3891818 0.01094583 0.00000000 0.05456667 0.05456667 9.367566e-01 5.512263e-14 FALSE TRUE
ENST00000497064 ENSG00000169155 HEK293_OSMI2_6hA HEK293_TMG_6hB ZBTB43 protein_coding processed_transcript 5.494604 3.65664 7.001392 0.6967604 0.2887198 0.9352423 1.13306884 1.2237542 0.0000000 0.2935672 0.0000000 -6.9469112 0.24837083 0.33826667 0.00000000 -0.33826667 5.512263e-14 5.512263e-14   FALSE
MSTRG.33401.3 ENSG00000169155 HEK293_OSMI2_6hA HEK293_TMG_6hB ZBTB43 protein_coding   5.494604 3.65664 7.001392 0.6967604 0.2887198 0.9352423 0.06301603 0.3127800 0.0000000 0.3127800 0.0000000 -5.0124791 0.01201667 0.06486667 0.00000000 -0.06486667 7.998299e-01 5.512263e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000169155 E001 2.3587651 0.009544168 6.966566e-02 2.241257e-01 9 126805006 126805032 27 + 0.370 0.675 1.474
ENSG00000169155 E002 3.3206206 0.007696791 5.200902e-01 7.150352e-01 9 126805033 126805039 7 + 0.566 0.675 0.474
ENSG00000169155 E003 11.4974820 0.002734293 4.932296e-01 6.961175e-01 9 126805040 126805132 93 + 1.093 1.032 -0.222
ENSG00000169155 E004 6.2793088 0.095263041 1.826993e-01 4.075436e-01 9 126808793 126808795 3 + 0.918 0.666 -1.000
ENSG00000169155 E005 17.2251662 0.002175024 1.578366e-02 8.103600e-02 9 126808796 126808915 120 + 1.296 1.085 -0.750
ENSG00000169155 E006 0.9989696 0.015700715 9.530721e-02   9 126831409 126832486 1078 + 0.160 0.457 2.060
ENSG00000169155 E007 448.3618765 0.001124243 1.221260e-08 5.605351e-07 9 126832487 126836219 3733 + 2.647 2.582 -0.217
ENSG00000169155 E008 249.8514508 0.003129091 2.875412e-08 1.215393e-06 9 126836220 126838210 1991 + 2.314 2.467 0.511