ENSG00000169122

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000519262 ENSG00000169122 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM110B protein_coding protein_coding 3.562294 4.362095 2.533663 0.774642 0.1759653 -0.7814175 2.7828655 3.6555607 1.5484127 0.74302492 0.02967915 -1.23395652 0.76862500 0.8283 0.6165667 -0.21173333 0.03653095 0.03653095 FALSE TRUE
ENST00000520369 ENSG00000169122 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM110B protein_coding processed_transcript 3.562294 4.362095 2.533663 0.774642 0.1759653 -0.7814175 0.3507577 0.2509979 0.5338647 0.07177607 0.08426860 1.05920955 0.10992500 0.0641 0.2085333 0.14443333 0.08196319 0.03653095 TRUE FALSE
ENST00000523486 ENSG00000169122 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM110B protein_coding processed_transcript 3.562294 4.362095 2.533663 0.774642 0.1759653 -0.7814175 0.2149747 0.3313225 0.3147396 0.13467547 0.04370494 -0.07185217 0.06334583 0.0797 0.1276333 0.04793333 0.74749568 0.03653095   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000169122 E001 1.7495669 0.012782452 8.796676e-01 0.9410920938 8 57994523 57994535 13 + 0.386 0.438 0.282
ENSG00000169122 E002 4.2573457 0.006976359 9.917323e-02 0.2803151712 8 57994536 57994553 18 + 0.464 0.763 1.329
ENSG00000169122 E003 5.0777839 0.041516509 2.924818e-01 0.5331583874 8 57994554 57994567 14 + 0.589 0.808 0.915
ENSG00000169122 E004 18.1165008 0.002529343 9.373927e-01 0.9708916240 8 57994568 57994806 239 + 1.236 1.265 0.104
ENSG00000169122 E005 8.0118414 0.076370056 4.889556e-01 0.6932888967 8 58031606 58031615 10 + 0.998 0.898 -0.374
ENSG00000169122 E006 15.3251362 0.145738231 7.906531e-01 0.8908816006 8 58031616 58031703 88 + 1.235 1.162 -0.256
ENSG00000169122 E007 0.6979399 0.092583622 4.448643e-01   8 58043184 58043299 116 + 0.291 0.158 -1.117
ENSG00000169122 E008 0.0000000       8 58070207 58070226 20 +      
ENSG00000169122 E009 0.0000000       8 58070227 58070317 91 +      
ENSG00000169122 E010 0.0000000       8 58070318 58070362 45 +      
ENSG00000169122 E011 0.0000000       8 58070363 58070387 25 +      
ENSG00000169122 E012 20.8460364 0.007655450 5.496528e-02 0.1912675876 8 58075535 58075623 89 + 1.394 1.266 -0.445
ENSG00000169122 E013 0.3032425 0.027442404 5.596991e-01   8 58077127 58077214 88 + 0.000 0.157 9.270
ENSG00000169122 E014 208.5679489 0.008986233 2.262420e-03 0.0188110151 8 58145907 58148788 2882 + 2.211 2.316 0.350
ENSG00000169122 E015 2.3461411 0.009883211 3.049968e-01 0.5455773382 8 58189318 58189416 99 + 0.587 0.439 -0.710
ENSG00000169122 E016 7.8785291 0.011445463 1.292412e-02 0.0701472483 8 58197831 58198157 327 + 1.079 0.810 -1.008
ENSG00000169122 E017 7.7758978 0.005511543 8.193285e-06 0.0001763956 8 58203515 58204279 765 + 1.185 0.711 -1.791