ENSG00000169018

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000306917 ENSG00000169018 HEK293_OSMI2_6hA HEK293_TMG_6hB FEM1B protein_coding protein_coding 15.51505 3.557842 26.54051 0.8547327 1.194622 2.895616 12.4325607 2.7247412 21.261936 0.4987082 0.5679158 2.959475 0.73588750 0.8052333 0.8037 -0.001533333 9.721379e-01 3.816651e-06 FALSE TRUE
ENST00000566008 ENSG00000169018 HEK293_OSMI2_6hA HEK293_TMG_6hB FEM1B protein_coding protein_coding 15.51505 3.557842 26.54051 0.8547327 1.194622 2.895616 0.5669983 0.8098453 0.000000 0.4652428 0.0000000 -6.357280 0.14696667 0.1822333 0.0000 -0.182233333 5.039966e-02 3.816651e-06 FALSE TRUE
ENST00000566739 ENSG00000169018 HEK293_OSMI2_6hA HEK293_TMG_6hB FEM1B protein_coding protein_coding 15.51505 3.557842 26.54051 0.8547327 1.194622 2.895616 1.8914088 0.0000000 3.075998 0.0000000 0.7914441 8.269593 0.07628333 0.0000000 0.1141 0.114100000 8.314952e-06 3.816651e-06 FALSE FALSE
MSTRG.11108.2 ENSG00000169018 HEK293_OSMI2_6hA HEK293_TMG_6hB FEM1B protein_coding   15.51505 3.557842 26.54051 0.8547327 1.194622 2.895616 0.5479947 0.0000000 2.066664 0.0000000 0.2636305 7.698124 0.02742500 0.0000000 0.0774 0.077400000 3.816651e-06 3.816651e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000169018 E001 46.2012545 0.9361597200 2.995533e-01 0.53997751 15 68277745 68278306 562 + 1.429 1.807 1.288
ENSG00000169018 E002 66.7574622 1.0749870363 4.317936e-01 0.65258609 15 68278307 68278665 359 + 1.609 1.879 0.914
ENSG00000169018 E003 0.9528494 0.3236192012 3.159821e-01   15 68279959 68280298 340 + 0.168 0.441 1.900
ENSG00000169018 E004 0.3228314 0.3964104722 1.000000e+00   15 68289255 68289361 107 + 0.094 0.001 -6.759
ENSG00000169018 E005 286.3374153 0.0012099966 4.953571e-06 0.00011422 15 68289607 68290211 605 + 2.286 2.235 -0.170
ENSG00000169018 E006 197.7396592 0.0002264731 1.365556e-03 0.01265293 15 68290212 68290487 276 + 2.121 2.110 -0.036
ENSG00000169018 E007 171.4363178 0.0003571230 2.787466e-03 0.02211004 15 68290488 68290709 222 + 2.059 2.047 -0.043
ENSG00000169018 E008 1656.1295847 0.0131627202 1.725428e-01 0.39384579 15 68290710 68295862 5153 + 3.020 3.150 0.434