ENSG00000168661

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000303586 ENSG00000168661 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF30 protein_coding protein_coding 2.812285 1.32844 4.087341 0.2484599 0.3881908 1.614136 0.3753889 0.0000000 1.6442794 0.00000000 0.2222669 7.370059 0.1062667 0.00000000 0.4014000 0.40140000 3.347103e-10 3.347103e-10 FALSE TRUE
ENST00000595818 ENSG00000168661 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF30 protein_coding processed_transcript 2.812285 1.32844 4.087341 0.2484599 0.3881908 1.614136 0.2037326 0.3370286 0.0000000 0.33702856 0.0000000 -5.116982 0.1279542 0.22546667 0.0000000 -0.22546667 7.901527e-01 3.347103e-10 FALSE TRUE
ENST00000601142 ENSG00000168661 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF30 protein_coding protein_coding 2.812285 1.32844 4.087341 0.2484599 0.3881908 1.614136 0.5056649 0.1656236 0.0000000 0.16562358 0.0000000 -4.134415 0.1688708 0.10033333 0.0000000 -0.10033333 6.981617e-01 3.347103e-10 FALSE TRUE
ENST00000601957 ENSG00000168661 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF30 protein_coding protein_coding 2.812285 1.32844 4.087341 0.2484599 0.3881908 1.614136 0.8721766 0.6468760 1.8726658 0.21540996 0.4094729 1.519084 0.3255792 0.52716667 0.4465667 -0.08060000 9.341670e-01 3.347103e-10 FALSE TRUE
MSTRG.16961.5 ENSG00000168661 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF30 protein_coding   2.812285 1.32844 4.087341 0.2484599 0.3881908 1.614136 0.7841412 0.1207476 0.5012829 0.06417872 0.2520131 1.967337 0.2442083 0.07776667 0.1312000 0.05343333 9.786174e-01 3.347103e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000168661 E001 0.5173834 0.619341771 0.41147939   19 34926903 34926909 7 + 0.173 0.000 -10.195
ENSG00000168661 E002 1.7148037 0.011916063 0.01336507 0.07190125 19 34926910 34926926 17 + 0.438 0.000 -12.021
ENSG00000168661 E003 3.9577617 0.066463389 0.01820991 0.08971981 19 34926927 34926979 53 + 0.666 0.364 -1.468
ENSG00000168661 E004 12.4969896 0.004020416 0.04176901 0.15931627 19 34926980 34927216 237 + 1.053 1.035 -0.064
ENSG00000168661 E005 0.6238610 0.020267995 0.37759022   19 34927217 34927282 66 + 0.120 0.364 2.048
ENSG00000168661 E006 11.4230631 0.047448814 0.05879324 0.20004898 19 34929884 34929956 73 + 1.019 0.976 -0.159
ENSG00000168661 E007 16.8656347 0.108487330 0.19524428 0.42364931 19 34931843 34931993 151 + 1.153 1.200 0.167
ENSG00000168661 E008 0.1426347 0.031544297 1.00000000   19 34933611 34933624 14 + 0.064 0.000 -8.561
ENSG00000168661 E009 3.7305668 0.184815262 0.05902319 0.20056905 19 34933625 34933627 3 + 0.637 0.368 -1.320
ENSG00000168661 E010 15.4989646 0.030900756 0.28268839 0.52311171 19 34933628 34933723 96 + 1.108 1.219 0.397
ENSG00000168661 E011 9.3940422 0.003606057 0.06778858 0.22012428 19 34942594 34942673 80 + 0.942 0.915 -0.104
ENSG00000168661 E012 101.2696857 0.721476576 0.15541617 0.37006612 19 34943223 34945170 1948 + 1.811 2.179 1.234