ENSG00000168118

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366690 ENSG00000168118 HEK293_OSMI2_6hA HEK293_TMG_6hB RAB4A protein_coding protein_coding 25.80469 16.94138 28.95835 3.533023 2.049121 0.773075 18.866721 10.46141 24.646921 1.672337 1.081704 1.235537 0.7403458 0.6350333 0.85520000 0.2201667 0.03625472 0.0117249 FALSE TRUE
ENST00000481981 ENSG00000168118 HEK293_OSMI2_6hA HEK293_TMG_6hB RAB4A protein_coding retained_intron 25.80469 16.94138 28.95835 3.533023 2.049121 0.773075 5.195653 5.63911 1.826469 1.867468 1.013055 -1.621089 0.1884250 0.3123000 0.05953333 -0.2527667 0.01172490 0.0117249   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000168118 E001 0.0000000       1 229271062 229271074 13 +      
ENSG00000168118 E002 0.9693498 0.1579148378 3.712293e-02   1 229271075 229271110 36 + 0.096 0.534 3.291
ENSG00000168118 E003 36.6802008 0.4514259535 1.650338e-02 8.364880e-02 1 229271111 229271193 83 + 1.158 1.865 2.432
ENSG00000168118 E004 74.4312281 0.0319415317 7.752949e-06 1.683023e-04 1 229271194 229271275 82 + 1.653 2.083 1.450
ENSG00000168118 E005 139.9177362 0.0087871957 1.484335e-03 1.350591e-02 1 229271276 229271370 95 + 2.061 2.240 0.599
ENSG00000168118 E006 0.7379733 0.0204427969 6.082550e-02   1 229286485 229286485 1 + 0.096 0.427 2.750
ENSG00000168118 E007 155.0502924 0.0050531344 7.494777e-01 8.664594e-01 1 229286486 229286566 81 + 2.180 2.181 0.003
ENSG00000168118 E008 276.6645073 0.0003200793 7.826792e-02 2.413424e-01 1 229288729 229288843 115 + 2.440 2.416 -0.079
ENSG00000168118 E009 2.3547189 0.1901350856 7.565369e-01 8.705653e-01 1 229288844 229288882 39 + 0.545 0.482 -0.302
ENSG00000168118 E010 3.5308148 0.0069935611 8.763485e-01 9.394039e-01 1 229288966 229289199 234 + 0.651 0.639 -0.053
ENSG00000168118 E011 269.6385074 0.0002004709 1.318685e-02 7.120154e-02 1 229295848 229295910 63 + 2.435 2.397 -0.129
ENSG00000168118 E012 223.2162107 0.0010887421 5.654159e-01 7.469980e-01 1 229297482 229297519 38 + 2.338 2.337 -0.005
ENSG00000168118 E013 381.3873058 0.0002247998 9.213808e-01 9.629499e-01 1 229297520 229297636 117 + 2.564 2.581 0.057
ENSG00000168118 E014 325.0150494 0.0002090842 6.177708e-01 7.822692e-01 1 229298977 229299072 96 + 2.492 2.518 0.084
ENSG00000168118 E015 340.4706493 0.0001958930 1.199143e-01 3.157226e-01 1 229302862 229302989 128 + 2.506 2.551 0.150
ENSG00000168118 E016 613.6422132 0.0011445115 8.205984e-10 4.872604e-08 1 229303806 229305894 2089 + 2.812 2.710 -0.340