ENSG00000168061

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000398846 ENSG00000168061 HEK293_OSMI2_6hA HEK293_TMG_6hB SAC3D1 protein_coding protein_coding 84.47865 118.932 39.25246 25.94709 0.5358221 -1.599035 4.432908 7.49187 2.021613 1.977495 0.3076086 -1.884625 0.05232917 0.0614000 0.0515000 -0.0099000 8.077573e-01 1.722218e-09 FALSE TRUE
ENST00000533017 ENSG00000168061 HEK293_OSMI2_6hA HEK293_TMG_6hB SAC3D1 protein_coding processed_transcript 84.47865 118.932 39.25246 25.94709 0.5358221 -1.599035 8.438323 11.84406 4.974218 2.195011 0.3995727 -1.249941 0.09932500 0.1075333 0.1266333 0.0191000 8.612531e-01 1.722218e-09   FALSE
ENST00000652489 ENSG00000168061 HEK293_OSMI2_6hA HEK293_TMG_6hB SAC3D1 protein_coding protein_coding 84.47865 118.932 39.25246 25.94709 0.5358221 -1.599035 26.847401 39.26064 5.617411 9.823939 0.2112437 -2.802908 0.30904167 0.3240333 0.1431000 -0.1809333 1.722218e-09 1.722218e-09 FALSE TRUE
MSTRG.5695.2 ENSG00000168061 HEK293_OSMI2_6hA HEK293_TMG_6hB SAC3D1 protein_coding   84.47865 118.932 39.25246 25.94709 0.5358221 -1.599035 41.552067 55.61568 26.211565 11.778526 0.3848019 -1.084997 0.50257917 0.4685667 0.6678333 0.1992667 5.685272e-06 1.722218e-09   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000168061 E001 1.594428 0.0307177707 8.421206e-02 2.531893e-01 11 65040901 65040917 17 + 0.000 0.414 10.579
ENSG00000168061 E002 14.109478 0.0025791132 4.220093e-01 6.451519e-01 11 65040918 65040940 23 + 1.010 1.078 0.251
ENSG00000168061 E003 49.488068 0.0010467057 4.591049e-02 1.698322e-01 11 65040941 65040969 29 + 1.711 1.565 -0.495
ENSG00000168061 E004 106.444597 0.0004487195 3.386358e-04 4.126293e-03 11 65040970 65041106 137 + 2.051 1.878 -0.583
ENSG00000168061 E005 76.458957 0.0104770305 9.072017e-01 9.557317e-01 11 65041107 65041209 103 + 1.786 1.763 -0.077
ENSG00000168061 E006 84.501708 0.0007514444 3.148450e-04 3.879829e-03 11 65041210 65041244 35 + 1.674 1.843 0.574
ENSG00000168061 E007 137.703184 0.0046819439 4.057149e-06 9.610784e-05 11 65041245 65041285 41 + 1.831 2.055 0.754
ENSG00000168061 E008 413.319253 0.0017941159 7.932198e-18 1.772460e-15 11 65041286 65041866 581 + 2.298 2.527 0.764
ENSG00000168061 E009 5.849597 0.0057483362 4.834421e-02 1.757908e-01 11 65041867 65041928 62 + 0.978 0.681 -1.164
ENSG00000168061 E010 58.447136 0.0122613123 1.675484e-01 3.873395e-01 11 65043025 65043164 140 + 1.773 1.626 -0.499
ENSG00000168061 E011 1356.478488 0.0021496347 2.267452e-09 1.235769e-07 11 65044225 65044828 604 + 3.097 2.987 -0.365