ENSG00000165996

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000326961 ENSG00000165996 HEK293_OSMI2_6hA HEK293_TMG_6hB HACD1 protein_coding protein_coding 13.86738 8.320403 11.63331 1.437216 1.174606 0.4830436 1.210067 1.8600906 0.3494543 0.61341485 0.1020507 -2.3792278 0.09883333 0.21880000 0.02883333 -0.18996667 0.0008903627 0.0008903627 FALSE TRUE
ENST00000466335 ENSG00000165996 HEK293_OSMI2_6hA HEK293_TMG_6hB HACD1 protein_coding protein_coding 13.86738 8.320403 11.63331 1.437216 1.174606 0.4830436 6.599127 4.0003207 4.5926636 0.54456882 0.7769510 0.1987514 0.47363750 0.48706667 0.38970000 -0.09736667 0.5337196630 0.0008903627 FALSE TRUE
ENST00000471481 ENSG00000165996 HEK293_OSMI2_6hA HEK293_TMG_6hB HACD1 protein_coding retained_intron 13.86738 8.320403 11.63331 1.437216 1.174606 0.4830436 1.150488 0.5211937 1.3112514 0.07787435 0.1444463 1.3145950 0.08505833 0.06486667 0.11760000 0.05273333 0.5199459868 0.0008903627   FALSE
ENST00000498812 ENSG00000165996 HEK293_OSMI2_6hA HEK293_TMG_6hB HACD1 protein_coding nonsense_mediated_decay 13.86738 8.320403 11.63331 1.437216 1.174606 0.4830436 2.623158 0.9735815 3.0900259 0.64316757 0.9526041 1.6561638 0.17923333 0.09813333 0.25493333 0.15680000 0.6519144098 0.0008903627   FALSE
MSTRG.3613.2 ENSG00000165996 HEK293_OSMI2_6hA HEK293_TMG_6hB HACD1 protein_coding   13.86738 8.320403 11.63331 1.437216 1.174606 0.4830436 1.619205 0.5838326 1.7340963 0.29367408 0.5388506 1.5543515 0.11647083 0.08540000 0.16150000 0.07610000 0.8157743763 0.0008903627 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000165996 E001 3.142707 0.0134400207 6.645719e-01 0.8127522254 10 17589032 17590066 1035 - 0.646 0.592 -0.239
ENSG00000165996 E002 6.379174 0.0587731502 2.650840e-02 0.1167927132 10 17590067 17590274 208 - 0.986 0.631 -1.409
ENSG00000165996 E003 31.761764 0.0011052966 6.590468e-06 0.0001464172 10 17590275 17590446 172 - 1.608 1.344 -0.906
ENSG00000165996 E004 18.712022 0.0026337938 1.235182e-03 0.0116865768 10 17594205 17594208 4 - 1.384 1.138 -0.865
ENSG00000165996 E005 36.088700 0.0015006455 1.153156e-03 0.0110714847 10 17594209 17594383 175 - 1.629 1.457 -0.586
ENSG00000165996 E006 6.444506 0.0079946018 2.279850e-01 0.4629459951 10 17599074 17599171 98 - 0.769 0.938 0.654
ENSG00000165996 E007 29.885890 0.0014042485 1.823821e-01 0.4071121007 10 17599172 17599289 118 - 1.437 1.537 0.346
ENSG00000165996 E008 65.495374 0.0006307134 3.132776e-01 0.5535158928 10 17599290 17599411 122 - 1.825 1.803 -0.074
ENSG00000165996 E009 1.516541 0.1611452734 4.989358e-01 0.7000611879 10 17602906 17603559 654 - 0.437 0.323 -0.656
ENSG00000165996 E010 44.454044 0.0013816468 7.823961e-01 0.8860994278 10 17603560 17603648 89 - 1.649 1.654 0.017
ENSG00000165996 E011 3.117194 0.0599521169 6.193812e-01 0.7834204855 10 17603649 17603725 77 - 0.554 0.668 0.503
ENSG00000165996 E012 27.439998 0.0020411568 7.816493e-01 0.8856199814 10 17603726 17603744 19 - 1.432 1.468 0.125
ENSG00000165996 E013 2.799972 0.0130813864 5.354454e-01 0.7259013282 10 17603745 17603929 185 - 0.518 0.631 0.514
ENSG00000165996 E014 45.938546 0.0009779435 9.846794e-01 0.9941749014 10 17603930 17604047 118 - 1.654 1.674 0.068
ENSG00000165996 E015 14.495157 0.0674664737 4.631682e-06 0.0001077193 10 17615477 17615964 488 - 0.697 1.446 2.758
ENSG00000165996 E016 47.738219 0.0090943578 4.746660e-05 0.0007957473 10 17617083 17617374 292 - 1.539 1.812 0.928