ENSG00000165868

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369209 ENSG00000165868 HEK293_OSMI2_6hA HEK293_TMG_6hB HSPA12A protein_coding protein_coding 8.648621 9.841755 7.509363 0.6113516 0.1635565 -0.3897702 2.6545631 1.4340320 2.5437849 1.43403204 0.40829638 0.8225343 0.31646250 0.14310000 0.34110000 0.19800000 0.295426898 0.005234468 FALSE TRUE
ENST00000453491 ENSG00000165868 HEK293_OSMI2_6hA HEK293_TMG_6hB HSPA12A protein_coding processed_transcript 8.648621 9.841755 7.509363 0.6113516 0.1635565 -0.3897702 0.4527911 0.8380963 0.3578954 0.09505215 0.04522540 -1.2049327 0.05173750 0.08543333 0.04746667 -0.03796667 0.358798564 0.005234468   FALSE
ENST00000481291 ENSG00000165868 HEK293_OSMI2_6hA HEK293_TMG_6hB HSPA12A protein_coding retained_intron 8.648621 9.841755 7.509363 0.6113516 0.1635565 -0.3897702 0.4925932 0.4803672 0.7053323 0.25145379 0.18031426 0.5447510 0.05970000 0.05240000 0.09333333 0.04093333 0.698160457 0.005234468 FALSE TRUE
ENST00000674291 ENSG00000165868 HEK293_OSMI2_6hA HEK293_TMG_6hB HSPA12A protein_coding retained_intron 8.648621 9.841755 7.509363 0.6113516 0.1635565 -0.3897702 1.0058127 1.0431103 0.5525437 0.11159560 0.22561027 -0.9046196 0.10990833 0.10586667 0.07280000 -0.03306667 0.766413807 0.005234468 FALSE TRUE
ENST00000674372 ENSG00000165868 HEK293_OSMI2_6hA HEK293_TMG_6hB HSPA12A protein_coding processed_transcript 8.648621 9.841755 7.509363 0.6113516 0.1635565 -0.3897702 0.2423595 1.4293839 0.5094920 1.42938393 0.50949201 -1.4702780 0.02468333 0.13236667 0.06510000 -0.06726667 1.000000000 0.005234468 FALSE TRUE
ENST00000674389 ENSG00000165868 HEK293_OSMI2_6hA HEK293_TMG_6hB HSPA12A protein_coding retained_intron 8.648621 9.841755 7.509363 0.6113516 0.1635565 -0.3897702 0.6400230 0.5156976 0.8155695 0.41648676 0.03258967 0.6511566 0.07597083 0.05773333 0.10873333 0.05100000 0.606595728 0.005234468 FALSE TRUE
MSTRG.4696.27 ENSG00000165868 HEK293_OSMI2_6hA HEK293_TMG_6hB HSPA12A protein_coding   8.648621 9.841755 7.509363 0.6113516 0.1635565 -0.3897702 0.9784604 1.3015370 0.2015653 0.26147859 0.08080295 -2.6320833 0.10589583 0.13020000 0.02700000 -0.10320000 0.005234468 0.005234468 FALSE TRUE
MSTRG.4696.4 ENSG00000165868 HEK293_OSMI2_6hA HEK293_TMG_6hB HSPA12A protein_coding   8.648621 9.841755 7.509363 0.6113516 0.1635565 -0.3897702 0.3732756 0.7216498 0.1697969 0.17551897 0.10472079 -2.0247850 0.04444583 0.07503333 0.02256667 -0.05246667 0.438514280 0.005234468 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000165868 E001 0.3088520 0.0264941606 8.192761e-01   10 116671192 116671196 5 - 0.137 0.107 -0.413
ENSG00000165868 E002 4.4415155 0.0594754911 3.997555e-01 0.6280551221 10 116671197 116671210 14 - 0.640 0.797 0.646
ENSG00000165868 E003 655.7382193 0.0047591494 8.033780e-01 0.8982885459 10 116671211 116675418 4208 - 2.802 2.819 0.057
ENSG00000165868 E004 41.2866945 0.0087810403 8.281513e-01 0.9126900165 10 116676399 116676502 104 - 1.608 1.630 0.075
ENSG00000165868 E005 6.7304643 0.0727595911 5.956789e-02 0.2018033443 10 116676503 116678334 1832 - 0.639 0.994 1.398
ENSG00000165868 E006 55.5227452 0.0008484668 1.526567e-01 0.3661307782 10 116679503 116679761 259 - 1.782 1.725 -0.192
ENSG00000165868 E007 1.0287093 0.0241329234 1.864031e-01   10 116679762 116680079 318 - 0.137 0.381 1.915
ENSG00000165868 E008 35.3073473 0.0012786449 1.563510e-01 0.3713982099 10 116681152 116681256 105 - 1.594 1.521 -0.251
ENSG00000165868 E009 39.2455723 0.0045809660 7.942667e-02 0.2436727868 10 116681791 116681877 87 - 1.656 1.558 -0.332
ENSG00000165868 E010 3.7921032 0.0068377475 9.435072e-01 0.9740817154 10 116681878 116682024 147 - 0.673 0.692 0.077
ENSG00000165868 E011 3.5799334 0.1271995969 2.682822e-01 0.5079207017 10 116683076 116683455 380 - 0.514 0.735 0.968
ENSG00000165868 E012 3.6234648 0.0259312052 7.695591e-02 0.2387054347 10 116683456 116683790 335 - 0.457 0.760 1.349
ENSG00000165868 E013 23.4976014 0.0105816385 6.923377e-02 0.2232462282 10 116683791 116683812 22 - 1.456 1.315 -0.488
ENSG00000165868 E014 25.4903318 0.0042034521 2.735973e-02 0.1193695094 10 116683813 116683831 19 - 1.498 1.348 -0.518
ENSG00000165868 E015 42.9828549 0.0013837537 1.072526e-01 0.2944383868 10 116683832 116683958 127 - 1.683 1.607 -0.260
ENSG00000165868 E016 17.0575171 0.0023701264 4.549388e-02 0.1687930503 10 116683959 116683962 4 - 1.331 1.176 -0.547
ENSG00000165868 E017 43.4170385 0.0015397520 2.222288e-02 0.1033488391 10 116692351 116692467 117 - 1.703 1.589 -0.387
ENSG00000165868 E018 45.4520157 0.1037891833 5.547079e-01 0.7395657190 10 116692468 116694207 1740 - 1.675 1.650 -0.087
ENSG00000165868 E019 14.5700725 0.1666405037 2.969475e-01 0.5373998772 10 116697855 116698634 780 - 1.267 1.114 -0.543
ENSG00000165868 E020 36.7101860 0.0087005430 1.171410e-01 0.3110680979 10 116698635 116698739 105 - 1.622 1.523 -0.338
ENSG00000165868 E021 20.2933060 0.0068139878 1.903793e-01 0.4174384929 10 116700943 116700953 11 - 1.375 1.277 -0.340
ENSG00000165868 E022 44.0167253 0.0097233616 1.557100e-02 0.0802474386 10 116700954 116701129 176 - 1.731 1.579 -0.516
ENSG00000165868 E023 7.4627180 0.0069940176 1.778704e-03 0.0155575272 10 116703744 116703968 225 - 0.638 1.055 1.627
ENSG00000165868 E024 40.6525804 0.0094376882 5.374223e-01 0.7272475531 10 116705151 116705278 128 - 1.637 1.608 -0.097
ENSG00000165868 E025 0.3751086 0.0250579222 2.946943e-01   10 116705279 116705376 98 - 0.000 0.194 11.722
ENSG00000165868 E026 23.6756143 0.0116154633 9.112967e-01 0.9578514349 10 116707200 116707285 86 - 1.381 1.405 0.084
ENSG00000165868 E027 0.0000000       10 116707286 116707378 93 -      
ENSG00000165868 E028 0.2027342 0.0355076951 6.379152e-01   10 116708041 116708246 206 - 0.000 0.107 10.665
ENSG00000165868 E029 2.7174072 0.0225575744 2.530289e-02 0.1131447652 10 116727227 116727380 154 - 0.738 0.379 -1.681
ENSG00000165868 E030 0.1723744 0.0330834908 6.382128e-01   10 116742006 116742078 73 - 0.000 0.107 10.680
ENSG00000165868 E031 13.6410655 0.0424591607 8.152954e-01 0.9053737689 10 116742430 116742596 167 - 1.144 1.190 0.163
ENSG00000165868 E032 16.1549850 0.0121890327 1.024226e-03 0.0100997082 10 116826791 116828736 1946 - 0.996 1.344 1.244
ENSG00000165868 E033 10.8247548 0.0755374367 2.672669e-04 0.0033980738 10 116828764 116829075 312 - 0.559 1.232 2.614
ENSG00000165868 E034 68.4487645 0.0157795072 4.689631e-05 0.0007871192 10 116829076 116833066 3991 - 1.623 1.942 1.076
ENSG00000165868 E035 4.5888159 0.0105575222 4.858365e-01 0.6911311070 10 116833067 116833091 25 - 0.674 0.782 0.443
ENSG00000165868 E036 11.7319060 0.0153619382 5.112676e-02 0.1823686713 10 116833092 116833373 282 - 0.945 1.179 0.852
ENSG00000165868 E037 3.4625395 0.2778560191 3.836480e-01 0.6152650409 10 116833374 116834934 1561 - 0.461 0.741 1.252
ENSG00000165868 E038 5.4095910 0.0469022249 5.074286e-03 0.0350183082 10 116834935 116835022 88 - 0.456 0.948 2.081
ENSG00000165868 E039 3.0589609 0.0826462361 1.369951e-02 0.0731555296 10 116835198 116835257 60 - 0.241 0.746 2.622
ENSG00000165868 E040 0.8397668 0.0172671820 2.959765e-01   10 116847595 116848223 629 - 0.137 0.327 1.592
ENSG00000165868 E041 4.0477119 0.0064355341 5.493347e-03 0.0371829262 10 116849566 116849741 176 - 0.396 0.838 1.984
ENSG00000165868 E042 0.0000000       10 116850091 116850251 161 -