ENSG00000165832

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000298746 ENSG00000165832 HEK293_OSMI2_6hA HEK293_TMG_6hB TRUB1 protein_coding protein_coding 14.57054 2.926614 26.00596 0.767649 0.2992843 3.147172 12.869342 1.783383 24.301086 0.5011826 0.07265266 3.7608592 0.7883417 0.6006 0.93473333 0.3341333 1.783122e-07 1.783122e-07 FALSE TRUE
ENST00000485065 ENSG00000165832 HEK293_OSMI2_6hA HEK293_TMG_6hB TRUB1 protein_coding processed_transcript 14.57054 2.926614 26.00596 0.767649 0.2992843 3.147172 1.701196 1.143231 1.704878 0.2969111 0.36552176 0.5724242 0.2116583 0.3994 0.06526667 -0.3341333 1.842511e-07 1.783122e-07 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000165832 E001 70.896520 0.0036515363 1.656236e-01 3.846819e-01 10 114938195 114938385 191 + 1.591 1.698 0.364
ENSG00000165832 E002 109.634273 0.0013955624 1.859367e-01 4.117720e-01 10 114938386 114938539 154 + 1.780 1.862 0.276
ENSG00000165832 E003 108.375633 0.0004402604 2.736527e-01 5.135255e-01 10 114942645 114942743 99 + 1.788 1.727 -0.207
ENSG00000165832 E004 94.353548 0.0004668552 8.893996e-01 9.463944e-01 10 114951094 114951149 56 + 1.721 1.736 0.049
ENSG00000165832 E005 105.996419 0.0004352855 3.987026e-01 6.272401e-01 10 114959726 114959807 82 + 1.768 1.822 0.183
ENSG00000165832 E006 7.254055 0.0178160893 1.070477e-12 1.090393e-10 10 114959808 114960000 193 + 0.477 1.419 3.655
ENSG00000165832 E007 86.285090 0.0004808866 6.975471e-02 2.243085e-01 10 114970368 114970440 73 + 1.693 1.568 -0.424
ENSG00000165832 E008 132.609923 0.0003257218 7.010283e-04 7.451382e-03 10 114972135 114972274 140 + 1.880 1.680 -0.674
ENSG00000165832 E009 127.181682 0.0004163048 9.260678e-03 5.495420e-02 10 114974329 114974385 57 + 1.860 1.709 -0.509
ENSG00000165832 E010 724.321865 0.0003165534 2.491427e-01 4.869231e-01 10 114975123 114977676 2554 + 2.598 2.624 0.086