ENSG00000165731

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000340058 ENSG00000165731 HEK293_OSMI2_6hA HEK293_TMG_6hB RET protein_coding protein_coding 2.020409 2.479986 1.168167 0.1289465 0.2384413 -1.079593 0.17385348 0.36319648 0.0000000 0.18335858 0.00000000 -5.2218635 0.06865833 0.13926667 0.0000000 -0.13926667 0.4643454409 0.0009965458 FALSE TRUE
ENST00000355710 ENSG00000165731 HEK293_OSMI2_6hA HEK293_TMG_6hB RET protein_coding protein_coding 2.020409 2.479986 1.168167 0.1289465 0.2384413 -1.079593 0.47214145 0.57837592 0.4012542 0.13120612 0.12533705 -0.5167079 0.25992083 0.23890000 0.3932000 0.15430000 0.8589115294 0.0009965458 FALSE TRUE
ENST00000498820 ENSG00000165731 HEK293_OSMI2_6hA HEK293_TMG_6hB RET protein_coding protein_coding 2.020409 2.479986 1.168167 0.1289465 0.2384413 -1.079593 0.12710453 0.00000000 0.0000000 0.00000000 0.00000000 0.0000000 0.05385000 0.00000000 0.0000000 0.00000000   0.0009965458 FALSE FALSE
ENST00000671844 ENSG00000165731 HEK293_OSMI2_6hA HEK293_TMG_6hB RET protein_coding nonsense_mediated_decay 2.020409 2.479986 1.168167 0.1289465 0.2384413 -1.079593 0.04609617 0.11762519 0.0000000 0.11762519 0.00000000 -3.6738412 0.02156667 0.05273333 0.0000000 -0.05273333 0.9569732104 0.0009965458 FALSE TRUE
ENST00000683007 ENSG00000165731 HEK293_OSMI2_6hA HEK293_TMG_6hB RET protein_coding retained_intron 2.020409 2.479986 1.168167 0.1289465 0.2384413 -1.079593 0.10778714 0.04220908 0.2834038 0.04220908 0.05371298 2.4905148 0.06321250 0.01656667 0.2440000 0.22743333 0.0009965458 0.0009965458 FALSE TRUE
ENST00000684216 ENSG00000165731 HEK293_OSMI2_6hA HEK293_TMG_6hB RET protein_coding protein_coding 2.020409 2.479986 1.168167 0.1289465 0.2384413 -1.079593 0.22443587 0.26405378 0.1953959 0.13319192 0.14493781 -0.4160516 0.10443750 0.10126667 0.1315667 0.03030000 0.9955610814 0.0009965458 FALSE TRUE
MSTRG.3842.2 ENSG00000165731 HEK293_OSMI2_6hA HEK293_TMG_6hB RET protein_coding   2.020409 2.479986 1.168167 0.1289465 0.2384413 -1.079593 0.09488500 0.05491405 0.0000000 0.05491405 0.00000000 -2.6985308 0.05176250 0.02063333 0.0000000 -0.02063333 0.9667614219 0.0009965458 FALSE TRUE
MSTRG.3842.8 ENSG00000165731 HEK293_OSMI2_6hA HEK293_TMG_6hB RET protein_coding   2.020409 2.479986 1.168167 0.1289465 0.2384413 -1.079593 0.55865438 0.94391441 0.2613087 0.13001922 0.15205226 -1.8139244 0.26752917 0.37903333 0.2108000 -0.16823333 0.6926686073 0.0009965458 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000165731 E001 0.3032425 0.027442404 0.5518152269   10 43077064 43077068 5 + 0.000 0.150 10.662
ENSG00000165731 E002 0.3032425 0.027442404 0.5518152269   10 43077069 43077073 5 + 0.000 0.150 10.663
ENSG00000165731 E003 0.8092192 0.016960507 0.1212317774   10 43077074 43077085 12 + 0.000 0.308 11.987
ENSG00000165731 E004 4.1870048 0.053269824 0.1852638058 0.4109195493 10 43077086 43077331 246 + 0.479 0.718 1.070
ENSG00000165731 E005 2.7282642 0.012585908 0.9189225095 0.9616610156 10 43100459 43100483 25 + 0.546 0.516 -0.141
ENSG00000165731 E006 12.1573009 0.004040718 0.0185302109 0.0908157344 10 43100484 43100722 239 + 0.878 1.128 0.927
ENSG00000165731 E007 0.1723744 0.033159602 1.0000000000   10 43102302 43102322 21 + 0.000 0.082 9.666
ENSG00000165731 E008 0.0000000       10 43102323 43102341 19 +      
ENSG00000165731 E009 10.8291961 0.003095055 0.1641730002 0.3829192612 10 43102342 43102470 129 + 0.932 1.070 0.511
ENSG00000165731 E010 12.7131428 0.003222023 0.1551818935 0.3697121911 10 43102471 43102629 159 + 1.002 1.134 0.478
ENSG00000165731 E011 2.4161019 0.008716961 0.7886955325 0.8897474426 10 43102630 43102839 210 + 0.546 0.487 -0.282
ENSG00000165731 E012 0.4458772 0.021768165 0.9039397665   10 43104753 43104951 199 + 0.177 0.150 -0.283
ENSG00000165731 E013 8.8762163 0.006186067 0.3020373262 0.5426095933 10 43104952 43105193 242 + 0.877 0.986 0.410
ENSG00000165731 E014 7.3565506 0.004834694 0.4088488696 0.6351177586 10 43106376 43106571 196 + 0.815 0.905 0.347
ENSG00000165731 E015 8.3822073 0.031265588 0.7477678904 0.8653427721 10 43109031 43109206 176 + 0.929 0.946 0.063
ENSG00000165731 E016 5.1240396 0.006850619 0.3025734374 0.5431245652 10 43109207 43109230 24 + 0.655 0.790 0.556
ENSG00000165731 E017 10.5452976 0.003141946 0.6837745429 0.8251189793 10 43111207 43111465 259 + 1.002 1.030 0.102
ENSG00000165731 E018 11.1281346 0.003497116 0.3334187608 0.5719700411 10 43112099 43112224 126 + 0.980 1.070 0.328
ENSG00000165731 E019 11.9791751 0.005835483 0.1697579642 0.3901710494 10 43112853 43112963 111 + 0.980 1.113 0.486
ENSG00000165731 E020 12.4885639 0.004534949 0.7420510487 0.8616805604 10 43113556 43113675 120 + 1.082 1.099 0.062
ENSG00000165731 E021 0.7962354 0.018883156 0.6396149271   10 43113676 43114479 804 + 0.302 0.210 -0.698
ENSG00000165731 E022 19.0441040 0.010093718 0.8394434725 0.9190691047 10 43114480 43114736 257 + 1.269 1.267 -0.004
ENSG00000165731 E023 16.2919627 0.002589875 0.1482554711 0.3598231964 10 43116584 43116731 148 + 1.116 1.233 0.417
ENSG00000165731 E024 15.3892497 0.002489148 0.1913453300 0.4185591289 10 43118373 43118480 108 + 1.099 1.206 0.382
ENSG00000165731 E025 18.4422352 0.002192616 0.1469626561 0.3579541779 10 43119531 43119745 215 + 1.164 1.274 0.390
ENSG00000165731 E026 16.2286278 0.008129167 0.5013017132 0.7017190970 10 43120081 43120203 123 + 1.149 1.198 0.177
ENSG00000165731 E027 0.5893196 0.034122090 0.0044292472   10 43120204 43120912 709 + 0.478 0.000 -14.260
ENSG00000165731 E028 12.7664673 0.047502806 0.7639323120 0.8750271615 10 43121946 43122016 71 + 1.119 1.074 -0.165
ENSG00000165731 E029 15.6544865 0.026608759 0.4345819114 0.6546619352 10 43123671 43123808 138 + 1.119 1.200 0.289
ENSG00000165731 E030 14.9897753 0.003190187 0.4886506236 0.6930937514 10 43124883 43124982 100 + 1.220 1.140 -0.284
ENSG00000165731 E031 15.2680277 0.002527552 0.0761864345 0.2371134946 10 43126575 43126722 148 + 1.281 1.107 -0.619
ENSG00000165731 E032 14.4596642 0.002444127 0.3326516391 0.5713474861 10 43126723 43128111 1389 + 1.220 1.114 -0.379
ENSG00000165731 E033 7.4052631 0.004910869 0.0000483217 0.0008076905 10 43128112 43128285 174 + 1.164 0.675 -1.865
ENSG00000165731 E034 23.6503038 0.048158180 0.0048911518 0.0340370606 10 43128286 43129517 1232 + 1.566 1.219 -1.201
ENSG00000165731 E035 16.0746898 0.010308197 0.0102338631 0.0591167181 10 43129518 43130351 834 + 1.354 1.095 -0.914