ENSG00000165449

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000395347 ENSG00000165449 HEK293_OSMI2_6hA HEK293_TMG_6hB SLC16A9 protein_coding protein_coding 3.680807 1.097105 5.470707 0.1979657 0.09247377 2.30757 1.5848713 0.0000000 3.12838132 0.00000000 0.31205606 8.2938768 0.3687875 0.00000000 0.57050000 0.5705 4.788269e-11 4.788269e-11 FALSE TRUE
ENST00000395348 ENSG00000165449 HEK293_OSMI2_6hA HEK293_TMG_6hB SLC16A9 protein_coding protein_coding 3.680807 1.097105 5.470707 0.1979657 0.09247377 2.30757 1.9137972 0.9678838 2.12566367 0.19398130 0.16790858 1.1269495 0.5831708 0.87440000 0.38970000 -0.4847 3.721384e-06 4.788269e-11 FALSE TRUE
MSTRG.4018.4 ENSG00000165449 HEK293_OSMI2_6hA HEK293_TMG_6hB SLC16A9 protein_coding   3.680807 1.097105 5.470707 0.1979657 0.09247377 2.30757 0.1024595 0.0866253 0.09647103 0.04338872 0.05131174 0.1399881 0.0299000 0.09256667 0.01746667 -0.0751 5.741494e-01 4.788269e-11 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000165449 E001 0.000000       10 59650764 59650764 1 -      
ENSG00000165449 E002 136.299888 0.0004477172 0.375686836 0.60875793 10 59650765 59652723 1959 - 2.035 2.063 0.091
ENSG00000165449 E003 5.975172 0.0088215835 0.535683423 0.72608938 10 59652724 59652755 32 - 0.748 0.838 0.356
ENSG00000165449 E004 23.932908 0.0028084057 0.871451423 0.93673251 10 59652756 59652950 195 - 1.303 1.315 0.043
ENSG00000165449 E005 27.103405 0.0017180483 0.854055870 0.92712979 10 59653675 59653809 135 - 1.352 1.366 0.052
ENSG00000165449 E006 84.179248 0.0006898742 0.038053830 0.14976150 10 59653810 59654589 780 - 1.850 1.758 -0.312
ENSG00000165449 E007 21.378720 0.0016152897 0.237580109 0.47380150 10 59664227 59664322 96 - 1.278 1.168 -0.388
ENSG00000165449 E008 26.110799 0.0013991905 0.093796479 0.27078910 10 59672770 59672913 144 - 1.367 1.223 -0.504
ENSG00000165449 E009 11.183296 0.0028438473 0.934980868 0.96970090 10 59684096 59684097 2 - 0.996 1.007 0.040
ENSG00000165449 E010 17.793736 0.0018804154 0.988195627 0.99591541 10 59684098 59684197 100 - 1.185 1.187 0.008
ENSG00000165449 E011 14.590993 0.0022038627 0.069543606 0.22386332 10 59684198 59684327 130 - 1.061 1.239 0.640
ENSG00000165449 E012 1.116306 0.3185602814 0.667060689   10 59708606 59708783 178 - 0.259 0.354 0.631
ENSG00000165449 E013 13.810188 0.0029603271 0.008188338 0.05019739 10 59709479 59709850 372 - 1.010 1.271 0.937
ENSG00000165449 E014 0.000000       10 59735930 59736002 73 -