ENSG00000165410

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000298159 ENSG00000165410 HEK293_OSMI2_6hA HEK293_TMG_6hB CFL2 protein_coding protein_coding 39.73228 21.1336 63.0944 4.855502 3.190072 1.577519 2.56609 1.320858 3.167482 0.3641685 0.3363034 1.2555270 0.06253333 0.0607000 0.05006667 -0.01063333 0.7882491081 9.634105e-06 FALSE TRUE
ENST00000341223 ENSG00000165410 HEK293_OSMI2_6hA HEK293_TMG_6hB CFL2 protein_coding protein_coding 39.73228 21.1336 63.0944 4.855502 3.190072 1.577519 10.03952 2.289426 18.135324 0.7450554 0.4099066 2.9802521 0.21777083 0.1012667 0.28950000 0.18823333 0.0002382849 9.634105e-06 FALSE TRUE
ENST00000422678 ENSG00000165410 HEK293_OSMI2_6hA HEK293_TMG_6hB CFL2 protein_coding nonsense_mediated_decay 39.73228 21.1336 63.0944 4.855502 3.190072 1.577519 9.89271 5.622503 15.966769 1.0956646 1.4682561 1.5041276 0.25476667 0.2725000 0.25253333 -0.01996667 0.9041547569 9.634105e-06 TRUE FALSE
ENST00000673315 ENSG00000165410 HEK293_OSMI2_6hA HEK293_TMG_6hB CFL2 protein_coding protein_coding 39.73228 21.1336 63.0944 4.855502 3.190072 1.577519 14.36997 10.451812 20.702522 2.7325375 2.0281242 0.9853705 0.39864167 0.4845000 0.32693333 -0.15756667 0.0228860220 9.634105e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000165410 E001 23.6370562 0.0018463249 9.460249e-01 9.753744e-01 14 34709113 34710381 1269 - 1.333 1.346 0.045
ENSG00000165410 E002 2.0088685 0.0193636937 7.442734e-01 8.631987e-01 14 34710382 34710386 5 - 0.435 0.500 0.327
ENSG00000165410 E003 463.7794016 0.0034684486 8.522773e-14 1.075307e-11 14 34710387 34712007 1621 - 2.665 2.428 -0.790
ENSG00000165410 E004 64.4010050 0.0008592317 9.078786e-06 1.930129e-04 14 34712008 34712010 3 - 1.818 1.584 -0.792
ENSG00000165410 E005 78.4490336 0.0005917457 4.432717e-07 1.392743e-05 14 34712011 34712036 26 - 1.904 1.663 -0.814
ENSG00000165410 E006 257.3293011 0.0002945191 9.037940e-01 9.538029e-01 14 34712037 34712164 128 - 2.357 2.369 0.038
ENSG00000165410 E007 925.3391784 0.0036655722 4.064366e-05 6.976881e-04 14 34712165 34712851 687 - 2.886 2.982 0.320
ENSG00000165410 E008 153.6227952 0.0002821554 4.365979e-02 1.642691e-01 14 34712852 34712857 6 - 2.120 2.191 0.237
ENSG00000165410 E009 323.0323653 0.0001834314 6.653809e-02 2.172765e-01 14 34712858 34712977 120 - 2.445 2.493 0.157
ENSG00000165410 E010 275.7421135 0.0016736746 4.202000e-01 6.437808e-01 14 34713060 34713136 77 - 2.380 2.411 0.101
ENSG00000165410 E011 361.4338337 0.0002771657 6.515142e-01 8.045060e-01 14 34713254 34713396 143 - 2.503 2.520 0.058
ENSG00000165410 E012 299.9108493 0.0002013754 3.312948e-01 5.701406e-01 14 34713397 34713507 111 - 2.431 2.417 -0.047
ENSG00000165410 E013 178.6433561 0.0002699215 7.808470e-01 8.852074e-01 14 34713508 34713561 54 - 2.202 2.201 -0.002
ENSG00000165410 E014 0.3206185 0.0274424043 7.183348e-01   14 34713643 34713673 31 - 0.141 0.000 -8.992
ENSG00000165410 E015 4.0141048 0.0069709113 2.104555e-02 9.950215e-02 14 34713674 34713904 231 - 0.733 0.319 -2.022
ENSG00000165410 E016 2.0542032 0.0097958589 1.012100e-02 5.865538e-02 14 34714196 34714434 239 - 0.543 0.000 -11.692
ENSG00000165410 E017 2.4349212 0.0086439997 5.196283e-02 1.843119e-01 14 34714435 34714537 103 - 0.566 0.188 -2.304
ENSG00000165410 E018 138.5636107 0.0007767692 3.353065e-03 2.549714e-02 14 34714538 34714823 286 - 2.063 2.170 0.358