ENSG00000165300

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000683689 ENSG00000165300 HEK293_OSMI2_6hA HEK293_TMG_6hB SLITRK5 protein_coding protein_coding 5.692614 5.992581 7.127337 0.6628591 0.1950877 0.2498029 5.205 5.742362 6.286764 0.6194903 0.1730145 0.1304562 0.9187 0.9588333 0.8821 -0.07673333 0.008200204 0.008200204 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000165300 E001 48.694998 0.0287497006 7.120687e-01 8.431398e-01 13 87671371 87672209 839 + 1.686 1.714 0.092
ENSG00000165300 E002 12.671218 0.0101002545 2.237196e-02 1.038103e-01 13 87672615 87672825 211 + 0.997 1.230 0.838
ENSG00000165300 E003 2.878194 0.3291059396 5.464631e-01 7.337983e-01 13 87673485 87673574 90 + 0.661 0.491 -0.774
ENSG00000165300 E004 872.561276 0.0002924461 3.043357e-28 2.144532e-25 13 87675381 87680102 4722 + 2.914 2.966 0.175
ENSG00000165300 E005 44.252716 0.0198537457 3.332119e-11 2.602900e-09 13 87680103 87686078 5976 + 1.847 1.240 -2.082
ENSG00000165300 E006 3.280535 0.0811039852 2.020180e-01 4.321063e-01 13 87686079 87686120 42 + 0.731 0.479 -1.121
ENSG00000165300 E007 18.434593 0.0099517499 6.350979e-08 2.472587e-06 13 87686121 87688236 2116 + 1.469 0.941 -1.881
ENSG00000165300 E008 3.614721 0.0330999795 2.169426e-02 1.015710e-01 13 87688237 87688354 118 + 0.816 0.429 -1.718
ENSG00000165300 E009 10.149484 0.0032509206 6.334997e-04 6.870508e-03 13 87688355 87692464 4110 + 1.182 0.806 -1.393
ENSG00000165300 E010 37.610587 0.0010413819 5.631387e-12 5.078805e-10 13 87692465 87696272 3808 + 1.740 1.320 -1.439