ENSG00000165238

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000411624 ENSG00000165238 HEK293_OSMI2_6hA HEK293_TMG_6hB WNK2 protein_coding protein_coding 21.91193 34.11934 13.11183 3.979064 0.6085266 -1.379043 1.5809593 2.8648682 0.6665981 0.5405289 0.21375053 -2.0871249 0.06731250 0.08286667 0.05266667 -0.030200000 6.480932e-01 8.324678e-06 FALSE TRUE
ENST00000432730 ENSG00000165238 HEK293_OSMI2_6hA HEK293_TMG_6hB WNK2 protein_coding protein_coding 21.91193 34.11934 13.11183 3.979064 0.6085266 -1.379043 2.0017997 3.3756406 1.3493184 0.3800320 0.23474700 -1.3165454 0.08192917 0.09900000 0.10310000 0.004100000 1.000000e+00 8.324678e-06 FALSE TRUE
ENST00000460335 ENSG00000165238 HEK293_OSMI2_6hA HEK293_TMG_6hB WNK2 protein_coding processed_transcript 21.91193 34.11934 13.11183 3.979064 0.6085266 -1.379043 3.7521708 5.8538236 0.2343000 1.4624156 0.23429998 -4.5851160 0.13361667 0.16683333 0.01690000 -0.149933333 9.424510e-02 8.324678e-06   FALSE
ENST00000467401 ENSG00000165238 HEK293_OSMI2_6hA HEK293_TMG_6hB WNK2 protein_coding retained_intron 21.91193 34.11934 13.11183 3.979064 0.6085266 -1.379043 2.5187184 3.4948899 1.7022824 0.2623039 0.07048479 -1.0334485 0.11480000 0.10613333 0.13013333 0.024000000 7.862277e-01 8.324678e-06 FALSE TRUE
ENST00000471076 ENSG00000165238 HEK293_OSMI2_6hA HEK293_TMG_6hB WNK2 protein_coding retained_intron 21.91193 34.11934 13.11183 3.979064 0.6085266 -1.379043 1.1691134 1.5670855 0.5374264 0.5869485 0.05226605 -1.5265239 0.05543750 0.05030000 0.04143333 -0.008866667 1.000000e+00 8.324678e-06   FALSE
ENST00000478583 ENSG00000165238 HEK293_OSMI2_6hA HEK293_TMG_6hB WNK2 protein_coding processed_transcript 21.91193 34.11934 13.11183 3.979064 0.6085266 -1.379043 1.3819372 1.1293538 1.3797903 1.1293538 0.83389322 0.2866514 0.07538333 0.03993333 0.10023333 0.060300000 6.271864e-01 8.324678e-06 FALSE FALSE
ENST00000693671 ENSG00000165238 HEK293_OSMI2_6hA HEK293_TMG_6hB WNK2 protein_coding processed_transcript 21.91193 34.11934 13.11183 3.979064 0.6085266 -1.379043 0.6529952 0.4230333 1.7079812 0.1179082 0.09888290 1.9881643 0.04525000 0.01346667 0.13160000 0.118133333 8.324678e-06 8.324678e-06 FALSE TRUE
MSTRG.33011.19 ENSG00000165238 HEK293_OSMI2_6hA HEK293_TMG_6hB WNK2 protein_coding   21.91193 34.11934 13.11183 3.979064 0.6085266 -1.379043 2.7623401 4.6762531 0.4420893 1.8774249 0.44208934 -3.3737551 0.11332500 0.12863333 0.03256667 -0.096066667 3.184275e-01 8.324678e-06 FALSE TRUE
MSTRG.33011.9 ENSG00000165238 HEK293_OSMI2_6hA HEK293_TMG_6hB WNK2 protein_coding   21.91193 34.11934 13.11183 3.979064 0.6085266 -1.379043 1.3335116 2.2102212 1.1992361 0.2514705 0.30102450 -0.8766075 0.07137917 0.06613333 0.09380000 0.027666667 8.366593e-01 8.324678e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000165238 E001 0.3336024 0.0244411696 7.456656e-01   9 93184122 93184138 17 + 0.000 0.138 11.688
ENSG00000165238 E002 0.1308682 0.0326491905 1.000000e+00   9 93184139 93184145 7 + 0.000 0.074 10.678
ENSG00000165238 E003 0.6064850 0.0231718018 2.936211e-01   9 93184146 93184157 12 + 0.000 0.241 12.679
ENSG00000165238 E004 1.1124618 0.0153787590 9.697354e-02   9 93184158 93184165 8 + 0.000 0.362 13.488
ENSG00000165238 E005 4.9151303 0.0102620001 1.242192e-03 1.173524e-02 9 93184166 93184211 46 + 0.193 0.828 3.360
ENSG00000165238 E006 5.9088705 0.0505537906 3.132737e-02 1.312894e-01 9 93184212 93184247 36 + 0.429 0.875 1.948
ENSG00000165238 E007 9.5501339 0.0917907815 1.647950e-01 3.837360e-01 9 93184248 93184361 114 + 0.743 1.026 1.087
ENSG00000165238 E008 5.0327696 0.1332796366 2.591428e-01 4.980411e-01 9 93184362 93184385 24 + 0.513 0.790 1.194
ENSG00000165238 E009 3.3800795 0.0247053248 2.516156e-01 4.896366e-01 9 93184928 93184929 2 + 0.428 0.654 1.063
ENSG00000165238 E010 96.6416376 0.0004733734 2.951729e-09 1.557543e-07 9 93184930 93185610 681 + 2.081 1.867 -0.721
ENSG00000165238 E011 0.0000000       9 93204975 93205003 29 +      
ENSG00000165238 E012 108.1850650 0.0003542056 1.165050e-09 6.722808e-08 9 93229696 93229868 173 + 2.125 1.918 -0.694
ENSG00000165238 E013 137.6634313 0.0007540188 1.110282e-08 5.150365e-07 9 93230888 93231108 221 + 2.211 2.029 -0.609
ENSG00000165238 E014 0.5363366 0.0428909058 4.493938e-01   9 93234776 93234807 32 + 0.000 0.193 12.272
ENSG00000165238 E015 114.0017102 0.0004681767 2.743339e-10 1.770741e-08 9 93234808 93234922 115 + 2.150 1.937 -0.713
ENSG00000165238 E016 69.0490793 0.0005684593 4.919838e-05 8.204062e-04 9 93234923 93234965 43 + 1.908 1.734 -0.585
ENSG00000165238 E017 99.1048313 0.0004288840 2.357764e-06 6.025622e-05 9 93238233 93238321 89 + 2.061 1.892 -0.565
ENSG00000165238 E018 138.0623301 0.0002924404 6.887484e-06 1.517847e-04 9 93239757 93239976 220 + 2.180 2.046 -0.448
ENSG00000165238 E019 121.5839173 0.0077232060 2.686345e-01 5.083086e-01 9 93247543 93247834 292 + 2.061 2.011 -0.167
ENSG00000165238 E020 71.2558241 0.0037441318 4.408054e-02 1.653291e-01 9 93252883 93253082 200 + 1.695 1.824 0.434
ENSG00000165238 E021 28.9804485 0.0012337226 2.777122e-04 3.505941e-03 9 93256299 93256454 156 + 1.159 1.480 1.123
ENSG00000165238 E022 0.0000000       9 93256726 93256769 44 +      
ENSG00000165238 E023 48.9868496 0.0008305589 2.279332e-01 4.628999e-01 9 93256948 93257139 192 + 1.578 1.664 0.293
ENSG00000165238 E024 131.4344223 0.0075419621 8.586627e-01 9.296823e-01 9 93258931 93259614 684 + 2.046 2.068 0.073
ENSG00000165238 E025 120.2101053 0.0029724601 1.029390e-01 2.870322e-01 9 93261814 93262107 294 + 2.065 2.010 -0.185
ENSG00000165238 E026 71.3083051 0.0009297963 7.582553e-03 4.740785e-02 9 93262670 93262719 50 + 1.883 1.773 -0.371
ENSG00000165238 E027 0.1723744 0.0426157330 1.000000e+00   9 93263266 93263324 59 + 0.000 0.074 10.684
ENSG00000165238 E028 107.7301332 0.0003940012 5.951363e-02 2.017088e-01 9 93263566 93263734 169 + 2.019 1.960 -0.196
ENSG00000165238 E029 103.4279232 0.0004855305 1.140956e-01 3.059064e-01 9 93263917 93264033 117 + 1.995 1.947 -0.161
ENSG00000165238 E030 0.0000000       9 93267020 93267244 225 +      
ENSG00000165238 E031 127.5192668 0.0065699411 7.324612e-01 8.556594e-01 9 93267746 93267916 171 + 2.026 2.054 0.097
ENSG00000165238 E032 70.9953455 0.0106660252 2.251158e-01 4.595374e-01 9 93268020 93268065 46 + 1.723 1.817 0.316
ENSG00000165238 E033 86.7398785 0.0015878014 4.240727e-01 6.465677e-01 9 93268627 93268746 120 + 1.846 1.898 0.174
ENSG00000165238 E034 0.3336024 0.0244411696 7.456656e-01   9 93268840 93268950 111 + 0.000 0.138 11.682
ENSG00000165238 E035 166.3428940 0.0019421318 1.168856e-01 3.106512e-01 9 93288788 93289186 399 + 2.106 2.179 0.244
ENSG00000165238 E036 204.0768920 0.0008878810 1.791005e-01 4.027675e-01 9 93289187 93289620 434 + 2.211 2.266 0.186
ENSG00000165238 E037 93.1366382 0.0017783807 7.112099e-01 8.425627e-01 9 93289978 93290047 70 + 1.890 1.917 0.093
ENSG00000165238 E038 39.7559667 0.0017792519 6.784281e-01 8.217387e-01 9 93292308 93292310 3 + 1.559 1.543 -0.054
ENSG00000165238 E039 106.9348557 0.0013221558 8.762045e-01 9.393157e-01 9 93292311 93292396 86 + 1.967 1.971 0.013
ENSG00000165238 E040 327.9511166 0.0027520401 1.828718e-01 4.077062e-01 9 93292491 93293132 642 + 2.412 2.467 0.185
ENSG00000165238 E041 75.1490931 0.0012581141 5.594569e-01 7.429268e-01 9 93293133 93293173 41 + 1.792 1.836 0.149
ENSG00000165238 E042 154.6524296 0.0054395085 5.350821e-01 7.256392e-01 9 93297853 93298067 215 + 2.095 2.143 0.160
ENSG00000165238 E043 79.3789481 0.0023736183 2.111324e-01 4.431097e-01 9 93299070 93299261 192 + 1.784 1.863 0.266
ENSG00000165238 E044 35.6301507 0.0019924631 4.385426e-01 6.576814e-01 9 93300051 93300149 99 + 1.453 1.523 0.239
ENSG00000165238 E045 9.9720010 0.0033996194 4.927006e-01 6.958046e-01 9 93300150 93300326 177 + 0.917 1.013 0.356
ENSG00000165238 E046 23.2706496 0.0302285628 7.517184e-01 8.678033e-01 9 93306637 93306776 140 + 1.347 1.320 -0.096
ENSG00000165238 E047 33.4430623 0.0011566122 1.084275e-01 2.964417e-01 9 93306777 93306821 45 + 1.379 1.509 0.448
ENSG00000165238 E048 115.3428569 0.0357961885 3.989390e-01 6.273405e-01 9 93306822 93308327 1506 + 2.058 1.981 -0.261
ENSG00000165238 E049 164.3146904 0.0003352880 2.777786e-08 1.179920e-06 9 93308328 93308500 173 + 1.995 2.202 0.694
ENSG00000165238 E050 100.4724501 0.0008966642 2.586208e-06 6.524029e-05 9 93308501 93308584 84 + 1.763 1.995 0.779
ENSG00000165238 E051 72.0808191 0.0232624131 9.461269e-03 5.578769e-02 9 93308721 93309195 475 + 1.606 1.850 0.826
ENSG00000165238 E052 62.9871791 0.1569267742 9.207317e-01 9.625855e-01 9 93315488 93317519 2032 + 1.668 1.763 0.324
ENSG00000165238 E053 93.5614278 0.0004794613 4.484311e-04 5.208886e-03 9 93317520 93317631 112 + 1.784 1.956 0.581
ENSG00000165238 E054 23.4493189 0.0089625802 2.365442e-01 4.725902e-01 9 93317632 93317872 241 + 1.234 1.354 0.419
ENSG00000165238 E055 35.4397047 0.0208362791 5.843935e-02 1.992801e-01 9 93317873 93318027 155 + 1.334 1.541 0.713
ENSG00000165238 E056 183.9390645 0.0196925398 6.497409e-03 4.218894e-02 9 93318028 93319208 1181 + 2.044 2.245 0.671
ENSG00000165238 E057 48.7058345 0.0009417060 1.621292e-01 3.799494e-01 9 93320367 93320572 206 + 1.565 1.664 0.337