ENSG00000164654

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000340080 ENSG00000164654 HEK293_OSMI2_6hA HEK293_TMG_6hB MIOS protein_coding protein_coding 9.349286 3.493769 15.8156 0.5029456 0.8458784 2.17528 0.86775744 0.6611021 0.5986718 0.18212179 0.38961063 -0.1408679 0.13702083 0.2088333 0.04003333 -0.16880000 1.966246e-01 8.781697e-17 FALSE TRUE
ENST00000405785 ENSG00000164654 HEK293_OSMI2_6hA HEK293_TMG_6hB MIOS protein_coding protein_coding 9.349286 3.493769 15.8156 0.5029456 0.8458784 2.17528 1.63105071 1.1457529 1.3210874 0.44377958 0.33194264 0.2037724 0.19283750 0.3031000 0.08363333 -0.21946667 2.378003e-01 8.781697e-17 FALSE TRUE
ENST00000433056 ENSG00000164654 HEK293_OSMI2_6hA HEK293_TMG_6hB MIOS protein_coding protein_coding 9.349286 3.493769 15.8156 0.5029456 0.8458784 2.17528 0.06670616 0.2247834 0.0000000 0.22478344 0.00000000 -4.5532587 0.01630417 0.0522000 0.00000000 -0.05220000 6.985388e-01 8.781697e-17 FALSE FALSE
ENST00000433635 ENSG00000164654 HEK293_OSMI2_6hA HEK293_TMG_6hB MIOS protein_coding protein_coding 9.349286 3.493769 15.8156 0.5029456 0.8458784 2.17528 0.91172822 0.1479644 1.4806963 0.08024480 0.25910448 3.2383150 0.08272917 0.0473000 0.09243333 0.04513333 6.580976e-01 8.781697e-17 FALSE FALSE
ENST00000445169 ENSG00000164654 HEK293_OSMI2_6hA HEK293_TMG_6hB MIOS protein_coding protein_coding 9.349286 3.493769 15.8156 0.5029456 0.8458784 2.17528 1.56822194 0.5650200 3.2640214 0.18183705 0.25642168 2.5093798 0.16064583 0.1567667 0.20626667 0.04950000 7.227839e-01 8.781697e-17 FALSE FALSE
ENST00000456533 ENSG00000164654 HEK293_OSMI2_6hA HEK293_TMG_6hB MIOS protein_coding protein_coding 9.349286 3.493769 15.8156 0.5029456 0.8458784 2.17528 0.97710652 0.3896574 1.6672389 0.04304257 0.16327034 2.0692525 0.07841667 0.1136000 0.10530000 -0.00830000 9.617870e-01 8.781697e-17 FALSE TRUE
MSTRG.29491.5 ENSG00000164654 HEK293_OSMI2_6hA HEK293_TMG_6hB MIOS protein_coding   9.349286 3.493769 15.8156 0.5029456 0.8458784 2.17528 0.66294543 0.0000000 0.4429939 0.00000000 0.07130951 5.5014198 0.08485417 0.0000000 0.02793333 0.02793333 1.742286e-02 8.781697e-17 FALSE TRUE
MSTRG.29491.7 ENSG00000164654 HEK293_OSMI2_6hA HEK293_TMG_6hB MIOS protein_coding   9.349286 3.493769 15.8156 0.5029456 0.8458784 2.17528 1.50331752 0.0000000 4.5344889 0.00000000 0.08362023 8.8279742 0.10274167 0.0000000 0.28816667 0.28816667 8.781697e-17 8.781697e-17 FALSE TRUE
MSTRG.29491.8 ENSG00000164654 HEK293_OSMI2_6hA HEK293_TMG_6hB MIOS protein_coding   9.349286 3.493769 15.8156 0.5029456 0.8458784 2.17528 0.74199938 0.1433187 2.1699732 0.14331871 0.52776337 3.8297047 0.07200417 0.0557000 0.13460000 0.07890000 4.292226e-01 8.781697e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000164654 E001 0.1723744 0.0371199908 1.330355e-01   7 7566875 7566883 9 + 0.000 0.201 10.682
ENSG00000164654 E002 2.9123373 0.0082329632 1.120430e-07 4.105173e-06 7 7566884 7566960 77 + 0.185 0.992 4.057
ENSG00000164654 E003 3.9659847 0.0077625418 9.994576e-10 5.834682e-08 7 7566961 7566987 27 + 0.232 1.107 4.059
ENSG00000164654 E004 8.3168306 0.0045569338 3.641981e-07 1.169836e-05 7 7566988 7567052 65 + 0.704 1.270 2.118
ENSG00000164654 E005 9.7468583 0.0030380681 1.394314e-05 2.795262e-04 7 7567053 7567072 20 + 0.812 1.271 1.687
ENSG00000164654 E006 3.9350300 0.0789309276 1.966333e-03 1.684384e-02 7 7567073 7567155 83 + 0.416 0.971 2.377
ENSG00000164654 E007 3.6203809 0.0732944543 1.259187e-04 1.810350e-03 7 7567156 7567196 41 + 0.315 0.999 3.074
ENSG00000164654 E008 5.9711196 0.0045009075 8.891828e-04 9.006542e-03 7 7567197 7567296 100 + 0.639 1.064 1.657
ENSG00000164654 E009 13.8706868 0.0415043798 9.040679e-01 9.539484e-01 7 7567297 7567482 186 + 1.114 1.086 -0.103
ENSG00000164654 E010 7.8322135 0.1227123814 5.887301e-01 7.627995e-01 7 7567483 7567484 2 + 0.915 0.761 -0.599
ENSG00000164654 E011 8.0589429 0.0038003372 9.980995e-02 2.814123e-01 7 7567485 7567606 122 + 0.943 0.709 -0.917
ENSG00000164654 E012 16.4312905 0.0025326839 6.936390e-01 8.314460e-01 7 7567607 7567688 82 + 1.160 1.196 0.130
ENSG00000164654 E013 36.5954019 0.0015830524 6.264348e-01 7.881856e-01 7 7568026 7568123 98 + 1.493 1.524 0.107
ENSG00000164654 E014 145.7916380 0.0075314994 3.113678e-02 1.307423e-01 7 7572436 7572925 490 + 2.118 2.003 -0.387
ENSG00000164654 E015 206.5049923 0.0003699360 2.976907e-05 5.361622e-04 7 7572926 7573769 844 + 2.274 2.153 -0.404
ENSG00000164654 E016 66.0408572 0.0006079468 1.375659e-01 3.437988e-01 7 7574098 7574196 99 + 1.775 1.698 -0.261
ENSG00000164654 E017 1.2131104 0.0203370511 7.367599e-01   7 7581729 7581844 116 + 0.275 0.338 0.414
ENSG00000164654 E018 2.2303340 0.0372776794 4.534904e-01 6.683334e-01 7 7582630 7582819 190 + 0.493 0.339 -0.839
ENSG00000164654 E019 1.4052459 0.0857344198 1.225181e-01 3.197884e-01 7 7582954 7583117 164 + 0.232 0.532 1.767
ENSG00000164654 E020 102.7174735 0.0004431610 7.161164e-01 8.456859e-01 7 7583118 7583372 255 + 1.951 1.936 -0.051
ENSG00000164654 E021 85.7879683 0.0045409725 1.139912e-01 3.057705e-01 7 7585636 7585805 170 + 1.889 1.801 -0.297
ENSG00000164654 E022 57.2511554 0.0164084175 7.257773e-01 8.516287e-01 7 7588498 7588563 66 + 1.703 1.668 -0.117
ENSG00000164654 E023 84.0655380 0.0018958524 1.483390e-01 3.599359e-01 7 7589405 7589563 159 + 1.874 1.802 -0.243
ENSG00000164654 E024 75.9988772 0.0016481558 7.725656e-01 8.802079e-01 7 7594980 7595132 153 + 1.811 1.822 0.039
ENSG00000164654 E025 95.6981288 0.0004717686 5.871158e-02 1.998913e-01 7 7596257 7596461 205 + 1.898 1.973 0.251
ENSG00000164654 E026 1.4277311 0.0125111628 3.950320e-02 1.534681e-01 7 7605118 7605941 824 + 0.232 0.595 2.054
ENSG00000164654 E027 88.3685870 0.0004969290 1.113638e-01 3.012504e-01 7 7605942 7606071 130 + 1.867 1.933 0.221
ENSG00000164654 E028 89.7186410 0.0060434734 7.023544e-02 2.252285e-01 7 7606996 7608932 1937 + 1.865 1.965 0.337