ENSG00000164291

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380009 ENSG00000164291 HEK293_OSMI2_6hA HEK293_TMG_6hB ARSK protein_coding protein_coding 6.542951 2.250354 11.3844 0.2803754 0.3061836 2.333705 3.892104 0.829862 7.806169 0.1346929 0.2058789 3.218238 0.5017917 0.3646667 0.6857667 0.3211 7.794482e-07 3.316973e-07 FALSE TRUE
ENST00000504763 ENSG00000164291 HEK293_OSMI2_6hA HEK293_TMG_6hB ARSK protein_coding protein_coding 6.542951 2.250354 11.3844 0.2803754 0.3061836 2.333705 2.424470 1.402080 3.086452 0.1394231 0.1655899 1.132795 0.4683083 0.6279000 0.2713000 -0.3566 3.316973e-07 3.316973e-07   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000164291 E001 6.4084433 0.004251525 8.397679e-01 9.192601e-01 5 95555101 95555136 36 + 0.741 0.703 -0.157
ENSG00000164291 E002 12.4224788 0.002885115 6.177517e-01 7.822692e-01 5 95555137 95555166 30 + 0.972 1.030 0.214
ENSG00000164291 E003 17.3407246 0.002025443 7.139325e-01 8.443047e-01 5 95555167 95555182 16 + 1.109 1.144 0.128
ENSG00000164291 E004 36.2436103 0.003160781 1.690414e-02 8.510128e-02 5 95555183 95555356 174 + 1.388 1.563 0.602
ENSG00000164291 E005 27.2971641 0.001544311 5.701417e-02 1.960950e-01 5 95555357 95555404 48 + 1.273 1.429 0.542
ENSG00000164291 E006 45.2562399 0.001093537 1.322468e-20 4.197860e-18 5 95556208 95557057 850 + 1.392 1.909 1.756
ENSG00000164291 E007 29.6824864 0.001666297 7.055184e-02 2.258284e-01 5 95565998 95566127 130 + 1.352 1.169 -0.644
ENSG00000164291 E008 46.3689015 0.002100549 5.915459e-03 3.932594e-02 5 95567890 95568049 160 + 1.543 1.310 -0.805
ENSG00000164291 E009 1.3200360 0.013985914 2.741253e-01   5 95580892 95580942 51 + 0.308 0.001 -9.239
ENSG00000164291 E010 68.9643559 0.004523778 8.126007e-05 1.257961e-03 5 95582916 95583198 283 + 1.717 1.417 -1.027
ENSG00000164291 E011 42.0715994 0.011551305 2.308828e-02 1.062321e-01 5 95586562 95586733 172 + 1.505 1.275 -0.796
ENSG00000164291 E012 50.1706173 0.009032465 2.223029e-02 1.033774e-01 5 95591401 95591625 225 + 1.580 1.374 -0.709
ENSG00000164291 E013 0.9006742 0.246373786 3.399464e-02   5 95600625 95600683 59 + 0.100 0.546 3.283
ENSG00000164291 E014 56.7467987 0.004081734 1.998824e-01 4.295213e-01 5 95600847 95601071 225 + 1.621 1.523 -0.336
ENSG00000164291 E015 165.0321954 0.005651165 9.871277e-01 9.954000e-01 5 95603237 95605102 1866 + 2.065 2.060 -0.015