ENSG00000163946

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000459993 ENSG00000163946 HEK293_OSMI2_6hA HEK293_TMG_6hB TASOR protein_coding retained_intron 19.30027 5.70922 35.61636 1.161834 2.962208 2.639055 2.6857743 1.5715561 2.9449382 0.56155364 1.0478300 0.9017832 0.19097500 0.25810000 0.07956667 -0.17853333 1.957999e-01 3.085006e-05 FALSE TRUE
ENST00000478052 ENSG00000163946 HEK293_OSMI2_6hA HEK293_TMG_6hB TASOR protein_coding processed_transcript 19.30027 5.70922 35.61636 1.161834 2.962208 2.639055 1.1015567 0.0000000 2.7156153 0.00000000 0.9506041 8.0904382 0.03102083 0.00000000 0.07346667 0.07346667 3.085006e-05 3.085006e-05 FALSE FALSE
ENST00000485156 ENSG00000163946 HEK293_OSMI2_6hA HEK293_TMG_6hB TASOR protein_coding processed_transcript 19.30027 5.70922 35.61636 1.161834 2.962208 2.639055 4.6575946 1.5925935 8.4499672 0.45088847 1.4049321 2.4002435 0.27185000 0.27140000 0.23410000 -0.03730000 8.450486e-01 3.085006e-05 FALSE FALSE
ENST00000493960 ENSG00000163946 HEK293_OSMI2_6hA HEK293_TMG_6hB TASOR protein_coding protein_coding 19.30027 5.70922 35.61636 1.161834 2.962208 2.639055 4.0113794 0.8807999 6.7144837 0.05022504 0.8690649 2.9162501 0.15765833 0.17500000 0.19083333 0.01583333 9.456523e-01 3.085006e-05 FALSE TRUE
ENST00000614531 ENSG00000163946 HEK293_OSMI2_6hA HEK293_TMG_6hB TASOR protein_coding protein_coding 19.30027 5.70922 35.61636 1.161834 2.962208 2.639055 1.0343922 0.2243381 3.7722556 0.22433813 0.3388907 4.0125832 0.03947083 0.03406667 0.10750000 0.07343333 3.504008e-01 3.085006e-05 FALSE TRUE
ENST00000683822 ENSG00000163946 HEK293_OSMI2_6hA HEK293_TMG_6hB TASOR protein_coding protein_coding 19.30027 5.70922 35.61636 1.161834 2.962208 2.639055 2.5053812 0.3696526 5.0645293 0.36965260 0.5511766 3.7405222 0.13004167 0.05180000 0.14653333 0.09473333 3.769034e-01 3.085006e-05 FALSE TRUE
MSTRG.23131.2 ENSG00000163946 HEK293_OSMI2_6hA HEK293_TMG_6hB TASOR protein_coding   19.30027 5.70922 35.61636 1.161834 2.962208 2.639055 0.1846866 0.3359117 0.2130404 0.19654542 0.2130404 -0.6330989 0.01858333 0.08316667 0.00520000 -0.07796667 5.412063e-01 3.085006e-05 FALSE TRUE
MSTRG.23131.5 ENSG00000163946 HEK293_OSMI2_6hA HEK293_TMG_6hB TASOR protein_coding   19.30027 5.70922 35.61636 1.161834 2.962208 2.639055 1.8380331 0.2623089 3.2908287 0.26230890 0.3473099 3.5995122 0.09108750 0.03676667 0.09280000 0.05603333 4.417329e-01 3.085006e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000163946 E001 0.9854382 0.4289697480 1.000000e+00   3 56620132 56620132 1 - 0.220 0.253 0.262
ENSG00000163946 E002 94.1062608 0.0126234414 3.254660e-04 3.988768e-03 3 56620133 56620544 412 - 1.752 2.101 1.171
ENSG00000163946 E003 279.1769845 0.0130962115 2.591688e-07 8.672477e-06 3 56620545 56622737 2193 - 2.211 2.596 1.285
ENSG00000163946 E004 15.1435018 0.5198194706 1.628796e-01 3.810744e-01 3 56622738 56622858 121 - 0.954 1.433 1.707
ENSG00000163946 E005 2.8734422 0.1407637388 3.103428e-02 1.304344e-01 3 56622859 56622861 3 - 0.356 0.876 2.361
ENSG00000163946 E006 13.8255322 0.3238008394 1.368394e-02 7.309506e-02 3 56622862 56623003 142 - 0.845 1.518 2.414
ENSG00000163946 E007 44.0930122 0.8252546804 3.055449e-01 5.462067e-01 3 56623004 56623116 113 - 1.433 1.772 1.154
ENSG00000163946 E008 195.7437813 1.5843763911 4.797998e-01 6.869313e-01 3 56623117 56623566 450 - 2.084 2.362 0.929
ENSG00000163946 E009 109.8082348 1.3974979676 5.175994e-01 7.133431e-01 3 56623567 56623843 277 - 1.850 2.064 0.721
ENSG00000163946 E010 112.8058472 1.4187065466 5.390636e-01 7.283654e-01 3 56624479 56624643 165 - 1.866 2.058 0.645
ENSG00000163946 E011 41.3513615 0.8441230278 4.678162e-01 6.785806e-01 3 56624828 56624834 7 - 1.445 1.611 0.570
ENSG00000163946 E012 91.3078618 1.3377120584 5.373690e-01 7.272345e-01 3 56624835 56625006 172 - 1.778 1.956 0.601
ENSG00000163946 E013 85.8603278 0.0004799834 6.853847e-01 8.260957e-01 3 56627037 56627145 109 - 1.769 1.884 0.387
ENSG00000163946 E014 74.7829166 0.0006198245 8.428278e-01 9.210406e-01 3 56627582 56627648 67 - 1.713 1.797 0.286
ENSG00000163946 E015 80.8716083 0.0005132289 7.920847e-01 8.916611e-01 3 56627649 56627741 93 - 1.748 1.830 0.277
ENSG00000163946 E016 97.8544276 0.0162739450 9.364709e-01 9.704551e-01 3 56628492 56628614 123 - 1.822 1.918 0.324
ENSG00000163946 E017 1.1412444 0.0487084497 4.511495e-01   3 56628615 56628640 26 - 0.216 0.418 1.334
ENSG00000163946 E018 2.7182150 0.0199592679 9.189720e-01 9.616871e-01 3 56628744 56629103 360 - 0.447 0.535 0.435
ENSG00000163946 E019 308.4482774 0.0171273303 6.884949e-01 8.281568e-01 3 56633044 56633843 800 - 2.320 2.400 0.269
ENSG00000163946 E020 56.6882502 0.0148057719 7.649235e-01 8.755894e-01 3 56633844 56633898 55 - 1.595 1.672 0.262
ENSG00000163946 E021 60.0427210 0.0046739215 6.633450e-02 2.168246e-01 3 56633899 56633966 68 - 1.634 1.602 -0.112
ENSG00000163946 E022 1.5111914 0.1068295117 6.825487e-01 8.243351e-01 3 56633967 56633972 6 - 0.309 0.259 -0.348
ENSG00000163946 E023 70.4144096 0.0072423128 2.718925e-02 1.188571e-01 3 56638706 56638765 60 - 1.706 1.649 -0.193
ENSG00000163946 E024 118.5060212 0.0112805477 9.462508e-03 5.578988e-02 3 56639986 56640130 145 - 1.930 1.849 -0.272
ENSG00000163946 E025 200.0695988 0.0122617190 1.614040e-02 8.229783e-02 3 56641349 56641752 404 - 2.152 2.099 -0.178
ENSG00000163946 E026 268.2315712 0.0266151789 1.612634e-01 3.786446e-01 3 56646522 56647223 702 - 2.274 2.251 -0.075
ENSG00000163946 E027 80.3600365 0.0043249714 7.349814e-04 7.741566e-03 3 56648822 56648893 72 - 1.769 1.643 -0.427
ENSG00000163946 E028 74.7197901 0.0034492692 1.475529e-05 2.939341e-04 3 56648985 56649057 73 - 1.747 1.545 -0.688
ENSG00000163946 E029 105.8032190 0.0296510999 3.814497e-03 2.810610e-02 3 56660731 56660834 104 - 1.893 1.703 -0.642
ENSG00000163946 E030 82.6791311 0.0293040062 2.831415e-03 2.238128e-02 3 56660914 56660968 55 - 1.790 1.572 -0.740
ENSG00000163946 E031 67.6397834 0.0327880829 6.137014e-03 4.040498e-02 3 56660969 56661017 49 - 1.704 1.490 -0.730
ENSG00000163946 E032 76.4287032 0.0432107394 5.892835e-02 2.003481e-01 3 56662385 56662490 106 - 1.748 1.621 -0.432
ENSG00000163946 E033 53.2030512 0.0280543832 1.197341e-02 6.634560e-02 3 56663541 56663572 32 - 1.600 1.415 -0.632
ENSG00000163946 E034 3.5409499 0.3162124906 2.854900e-01 5.260835e-01 3 56663573 56664331 759 - 0.564 0.260 -1.701
ENSG00000163946 E035 76.4961877 0.0181439891 4.028126e-03 2.932518e-02 3 56666260 56666384 125 - 1.752 1.590 -0.550
ENSG00000163946 E036 81.3127637 0.0067158608 1.761022e-04 2.404616e-03 3 56668397 56668558 162 - 1.780 1.609 -0.581
ENSG00000163946 E037 75.1855059 0.0090336574 9.699644e-04 9.674749e-03 3 56669700 56669791 92 - 1.744 1.591 -0.522
ENSG00000163946 E038 66.1934680 0.0090908573 2.608706e-03 2.101804e-02 3 56670073 56670145 73 - 1.688 1.545 -0.489
ENSG00000163946 E039 0.1723744 0.0539360837 7.668794e-02   3 56671352 56671599 248 - 0.000 0.258 11.916
ENSG00000163946 E040 70.1938270 0.0042987256 4.122045e-05 7.063042e-04 3 56671600 56671692 93 - 1.721 1.524 -0.670
ENSG00000163946 E041 68.3229403 0.0172421770 3.556136e-02 1.430872e-01 3 56673580 56673725 146 - 1.701 1.604 -0.332
ENSG00000163946 E042 80.4370178 0.4303321147 2.825885e-02 1.220504e-01 3 56682676 56683789 1114 - 1.587 2.228 2.158