ENSG00000163781

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000260810 ENSG00000163781 HEK293_OSMI2_6hA HEK293_TMG_6hB TOPBP1 protein_coding protein_coding 25.46247 10.49522 46.91824 2.370656 1.786811 2.15935 8.001312 4.9892652 12.066737 1.2628987 0.6452886 1.272443 0.4021625 0.4670667 0.2576667 -0.209400000 0.0002407591 0.0002407591 FALSE TRUE
ENST00000505804 ENSG00000163781 HEK293_OSMI2_6hA HEK293_TMG_6hB TOPBP1 protein_coding retained_intron 25.46247 10.49522 46.91824 2.370656 1.786811 2.15935 2.730187 1.2302285 5.535698 0.2422473 0.4997275 2.160763 0.1238417 0.1195000 0.1179333 -0.001566667 1.0000000000 0.0002407591   FALSE
ENST00000513818 ENSG00000163781 HEK293_OSMI2_6hA HEK293_TMG_6hB TOPBP1 protein_coding retained_intron 25.46247 10.49522 46.91824 2.370656 1.786811 2.15935 3.384504 1.4072927 6.536238 0.4042347 0.7120439 2.207528 0.1223875 0.1302333 0.1392333 0.009000000 0.9658040210 0.0002407591 FALSE TRUE
MSTRG.23733.5 ENSG00000163781 HEK293_OSMI2_6hA HEK293_TMG_6hB TOPBP1 protein_coding   25.46247 10.49522 46.91824 2.370656 1.786811 2.15935 4.105064 0.8850215 8.316047 0.4615680 0.4081762 3.217637 0.1103792 0.0688000 0.1776000 0.108800000 0.5879667038 0.0002407591 FALSE TRUE
MSTRG.23733.6 ENSG00000163781 HEK293_OSMI2_6hA HEK293_TMG_6hB TOPBP1 protein_coding   25.46247 10.49522 46.91824 2.370656 1.786811 2.15935 5.925526 1.4698007 11.193472 0.1916051 0.5408813 2.920471 0.1858000 0.1500333 0.2383667 0.088333333 0.1695981519 0.0002407591 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000163781 E001 2.5923910 0.0480664660 2.713194e-01 5.112793e-01 3 133598175 133598462 288 - 0.462 0.660 0.913
ENSG00000163781 E002 1.0172733 0.0194915921 2.430552e-01   3 133598463 133598555 93 - 0.229 0.446 1.371
ENSG00000163781 E003 1.7543189 0.0110556207 2.304359e-02 1.060926e-01 3 133598556 133598630 75 - 0.271 0.663 2.054
ENSG00000163781 E004 3.3621970 0.0078099229 1.098057e-01 2.987190e-01 3 133600238 133600270 33 - 0.512 0.764 1.093
ENSG00000163781 E005 93.3190927 0.0037574479 5.288991e-07 1.625982e-05 3 133600271 133600789 519 - 1.829 2.073 0.820
ENSG00000163781 E006 129.4176367 0.0027343029 3.316482e-10 2.107840e-08 3 133600790 133600987 198 - 1.965 2.224 0.865
ENSG00000163781 E007 65.4223115 0.0007416919 8.661078e-07 2.508197e-05 3 133600988 133601018 31 - 1.681 1.917 0.795
ENSG00000163781 E008 269.7401956 0.0002062512 9.162345e-12 7.926525e-10 3 133601019 133601393 375 - 2.314 2.477 0.544
ENSG00000163781 E009 200.3445150 0.0007921930 1.429763e-05 2.857222e-04 3 133608535 133608696 162 - 2.193 2.323 0.434
ENSG00000163781 E010 188.2493130 0.0002408829 4.213193e-07 1.333887e-05 3 133608873 133608962 90 - 2.165 2.310 0.485
ENSG00000163781 E011 236.1337432 0.0002164919 1.057878e-08 4.938210e-07 3 133611004 133611141 138 - 2.264 2.410 0.488
ENSG00000163781 E012 215.8225521 0.0002441320 4.074033e-07 1.293688e-05 3 133612389 133612552 164 - 2.228 2.364 0.456
ENSG00000163781 E013 81.7729470 0.0005117386 1.906419e-01 4.177162e-01 3 133616814 133616820 7 - 1.830 1.888 0.195
ENSG00000163781 E014 178.7766629 0.0002650755 5.048757e-02 1.808064e-01 3 133616821 133616925 105 - 2.168 2.226 0.196
ENSG00000163781 E015 206.2666391 0.0003777050 2.006440e-04 2.682990e-03 3 133617160 133617326 167 - 2.216 2.322 0.352
ENSG00000163781 E016 18.5424445 0.0041414190 2.159634e-01 4.485758e-01 3 133617327 133617526 200 - 1.242 1.114 -0.456
ENSG00000163781 E017 12.6133445 0.0025364682 6.347006e-02 2.105210e-01 3 133618133 133618212 80 - 1.107 0.881 -0.837
ENSG00000163781 E018 229.2648335 0.0002590161 7.666920e-03 4.778930e-02 3 133618213 133618433 221 - 2.269 2.340 0.237
ENSG00000163781 E019 237.4766527 0.0007788195 2.406653e-01 4.773420e-01 3 133620155 133620347 193 - 2.295 2.327 0.105
ENSG00000163781 E020 175.4389012 0.0031598522 9.347492e-01 9.695880e-01 3 133623091 133623193 103 - 2.173 2.166 -0.021
ENSG00000163781 E021 178.3325416 0.0002885588 1.158837e-01 3.089408e-01 3 133623311 133623457 147 - 2.168 2.215 0.158
ENSG00000163781 E022 152.4272006 0.0002844576 3.999360e-01 6.281871e-01 3 133624052 133624175 124 - 2.105 2.132 0.090
ENSG00000163781 E023 0.8048655 0.0216157068 2.500971e-01   3 133628107 133628361 255 - 0.271 0.000 -10.671
ENSG00000163781 E024 143.0658575 0.0019515934 4.163733e-01 6.409282e-01 3 133628362 133628471 110 - 2.093 2.059 -0.115
ENSG00000163781 E025 168.5408522 0.0003517926 4.142644e-01 6.392522e-01 3 133628560 133628733 174 - 2.152 2.177 0.084
ENSG00000163781 E026 221.7341709 0.0019469021 9.213682e-01 9.629480e-01 3 133637876 133638162 287 - 2.271 2.274 0.008
ENSG00000163781 E027 193.1963020 0.0009842524 5.877840e-02 2.000185e-01 3 133639959 133640170 212 - 2.226 2.160 -0.221
ENSG00000163781 E028 201.8212319 0.0002145096 8.109727e-04 8.373165e-03 3 133643200 133643372 173 - 2.254 2.149 -0.352
ENSG00000163781 E029 135.4283152 0.0029897205 2.287944e-02 1.055533e-01 3 133644020 133644096 77 - 2.085 1.977 -0.360
ENSG00000163781 E030 89.8781400 0.0008385628 5.884922e-02 2.001673e-01 3 133644097 133644112 16 - 1.905 1.814 -0.306
ENSG00000163781 E031 232.7982904 0.0006291164 1.984491e-03 1.696525e-02 3 133644113 133644363 251 - 2.316 2.218 -0.325
ENSG00000163781 E032 188.4675852 0.0033376496 1.221652e-03 1.159408e-02 3 133649383 133649604 222 - 2.232 2.083 -0.496
ENSG00000163781 E033 87.2503173 0.0196131703 9.438166e-03 5.569754e-02 3 133649605 133649633 29 - 1.913 1.676 -0.799
ENSG00000163781 E034 2.6143101 0.0091774778 7.290992e-01 8.536687e-01 3 133649634 133649779 146 - 0.513 0.447 -0.325
ENSG00000163781 E035 194.5955630 0.0005186528 6.196042e-05 1.000124e-03 3 133649780 133649943 164 - 2.241 2.106 -0.453
ENSG00000163781 E036 143.1415529 0.0003358229 1.265262e-03 1.189693e-02 3 133652463 133652547 85 - 2.108 1.987 -0.407
ENSG00000163781 E037 127.5050983 0.0004836643 3.681098e-03 2.737327e-02 3 133652548 133652614 67 - 2.058 1.941 -0.391
ENSG00000163781 E038 64.1442852 0.0010369982 1.367208e-01 3.424836e-01 3 133652615 133652629 15 - 1.756 1.674 -0.279
ENSG00000163781 E039 207.9407459 0.0003086726 8.734207e-07 2.524714e-05 3 133653345 133653524 180 - 2.277 2.120 -0.526
ENSG00000163781 E040 184.2761335 0.0003037662 3.183300e-11 2.496986e-09 3 133655290 133655486 197 - 2.237 2.005 -0.775
ENSG00000163781 E041 157.7703828 0.0014317517 4.927423e-09 2.482319e-07 3 133656676 133656857 182 - 2.173 1.931 -0.811
ENSG00000163781 E042 144.5350256 0.0008834516 2.391888e-10 1.565309e-08 3 133657798 133657941 144 - 2.138 1.874 -0.889
ENSG00000163781 E043 119.2720904 0.0003687823 3.007507e-08 1.264133e-06 3 133659016 133659150 135 - 2.050 1.810 -0.807
ENSG00000163781 E044 66.8685438 0.0005970998 5.995778e-03 3.970601e-02 3 133661044 133661129 86 - 1.790 1.639 -0.511
ENSG00000163781 E045 22.2854357 0.0024092069 5.823692e-01 7.586310e-01 3 133661130 133661134 5 - 1.313 1.264 -0.172
ENSG00000163781 E046 33.0592093 0.0011793579 7.703367e-01 8.788596e-01 3 133661769 133662184 416 - 1.456 1.474 0.064
ENSG00000163781 E047 1.5665054 0.7732305759 4.214085e-01 6.446695e-01 3 133662185 133662380 196 - 0.425 0.000 -11.610
ENSG00000163781 E048 0.1723744 0.0754865448 1.265118e-01   3 133671157 133671206 50 - 0.000 0.205 11.899