ENSG00000163659

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000295924 ENSG00000163659 HEK293_OSMI2_6hA HEK293_TMG_6hB TIPARP protein_coding protein_coding 5.461034 2.258481 7.842797 0.6814419 0.1609916 1.79148 4.3830033 1.8294711 4.756572 0.5232847 1.0786579 1.373661 0.7402375 0.863 0.6095000 -0.2535000 0.4463961996 0.0004775427 FALSE TRUE
ENST00000473702 ENSG00000163659 HEK293_OSMI2_6hA HEK293_TMG_6hB TIPARP protein_coding protein_coding 5.461034 2.258481 7.842797 0.6814419 0.1609916 1.79148 0.2063702 0.0000000 0.834565 0.0000000 0.1566426 6.400137 0.0246000 0.000 0.1072667 0.1072667 0.0004775427 0.0004775427 FALSE TRUE
ENST00000481853 ENSG00000163659 HEK293_OSMI2_6hA HEK293_TMG_6hB TIPARP protein_coding protein_coding 5.461034 2.258481 7.842797 0.6814419 0.1609916 1.79148 0.5248776 0.4290101 2.251660 0.4290101 1.2858346 2.365056 0.1101208 0.137 0.2832333 0.1462333 0.7759533483 0.0004775427 FALSE TRUE
ENST00000495891 ENSG00000163659 HEK293_OSMI2_6hA HEK293_TMG_6hB TIPARP protein_coding protein_coding 5.461034 2.258481 7.842797 0.6814419 0.1609916 1.79148 0.3394976 0.0000000 0.000000 0.0000000 0.0000000 0.000000 0.1234083 0.000 0.0000000 0.0000000   0.0004775427 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000163659 E001 0.0000000       3 156673235 156673361 127 +      
ENSG00000163659 E002 14.3388948 0.0427712299 1.580168e-01 0.3737657453 3 156674590 156674796 207 + 1.089 1.247 0.564
ENSG00000163659 E003 0.0000000       3 156674845 156675391 547 +      
ENSG00000163659 E004 0.1779838 0.0408339329 1.000000e+00   3 156675569 156675778 210 + 0.078 0.001 -6.671
ENSG00000163659 E005 0.0000000       3 156676664 156676694 31 +      
ENSG00000163659 E006 143.7943666 0.0003711779 2.158634e-05 0.0004075891 3 156677657 156678614 958 + 2.143 2.018 -0.418
ENSG00000163659 E007 0.0000000       3 156681166 156681189 24 +      
ENSG00000163659 E008 52.5226107 0.0009068175 3.947987e-01 0.6241530211 3 156694020 156694188 169 + 1.691 1.649 -0.142
ENSG00000163659 E009 42.1580984 0.0013697702 9.152616e-01 0.9597973903 3 156695865 156696025 161 + 1.590 1.600 0.033
ENSG00000163659 E010 54.6668030 0.0007666551 9.971124e-01 1.0000000000 3 156703424 156703702 279 + 1.698 1.703 0.017
ENSG00000163659 E011 219.8369934 0.0005836013 3.732457e-04 0.0044710243 3 156704684 156706770 2087 + 2.274 2.351 0.259