ENSG00000163536

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000446050 ENSG00000163536 HEK293_OSMI2_6hA HEK293_TMG_6hB SERPINI1 protein_coding protein_coding 5.807579 1.885366 11.09542 0.2161099 0.6565089 2.550715 5.0549201 1.827924 9.225469 0.2235304 0.2916257 2.329108 0.9070917 0.968 0.8341667 -0.1338333 5.677047e-03 1.659912e-06 FALSE TRUE
ENST00000472747 ENSG00000163536 HEK293_OSMI2_6hA HEK293_TMG_6hB SERPINI1 protein_coding protein_coding 5.807579 1.885366 11.09542 0.2161099 0.6565089 2.550715 0.6443242 0.000000 1.725779 0.0000000 0.2644289 7.439439 0.0689875 0.000 0.1539000 0.1539000 1.659912e-06 1.659912e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000163536 E001 0.5117739 0.0244411696 0.51816477   3 167735243 167735325 83 + 0.117 0.224 1.135
ENSG00000163536 E002 1.7409751 0.0619007294 0.95345933 0.9786706 3 167735721 167735722 2 + 0.379 0.369 -0.053
ENSG00000163536 E003 1.8836098 0.0321906421 0.85760079 0.9291393 3 167735723 167735724 2 + 0.405 0.370 -0.195
ENSG00000163536 E004 19.4681340 0.0333275629 0.51544879 0.7119486 3 167735725 167735812 88 + 1.234 1.154 -0.282
ENSG00000163536 E005 15.1961346 0.0058893567 0.85010353 0.9250271 3 167735813 167735823 11 + 1.118 1.140 0.078
ENSG00000163536 E006 1.5280020 0.0173261933 0.11960834 0.3152159 3 167735824 167736135 312 + 0.284 0.569 1.553
ENSG00000163536 E007 58.1275548 0.0007092071 0.01869005 0.0913618 3 167789111 167789378 268 + 1.694 1.558 -0.464
ENSG00000163536 E008 62.8820444 0.0009422138 0.05001804 0.1797223 3 167790372 167790602 231 + 1.721 1.612 -0.369
ENSG00000163536 E009 60.9550058 0.0007494218 0.65473363 0.8065636 3 167792590 167792784 195 + 1.696 1.672 -0.080
ENSG00000163536 E010 64.5730377 0.0007203289 0.34565543 0.5832521 3 167794620 167794824 205 + 1.704 1.752 0.162
ENSG00000163536 E011 0.0000000       3 167800046 167800122 77 +      
ENSG00000163536 E012 0.0000000       3 167807238 167807243 6 +      
ENSG00000163536 E013 40.1871449 0.0015762312 0.22166130 0.4554856 3 167807244 167807341 98 + 1.494 1.574 0.275
ENSG00000163536 E014 0.3336024 0.0274424043 0.01679739   3 167822936 167822985 50 + 0.000 0.372 12.355
ENSG00000163536 E015 30.6928770 0.0013701125 0.80595997 0.8996923 3 167822986 167823072 87 + 1.396 1.415 0.068
ENSG00000163536 E016 33.8269219 0.0010736189 0.35512891 0.5918937 3 167824473 167824562 90 + 1.431 1.497 0.226
ENSG00000163536 E017 33.3933229 0.0010877325 0.05384648 0.1887242 3 167825247 167825569 323 + 1.409 1.541 0.457