ENSG00000163535

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000357799 ENSG00000163535 HEK293_OSMI2_6hA HEK293_TMG_6hB SGO2 protein_coding protein_coding 7.620953 1.718656 16.53414 0.2702612 0.6996098 3.258598 2.8623618 0.6831071 6.3751156 0.3445206 0.91771242 3.2035624 0.4829042 0.4520333 0.38700000 -0.06503333 1.000000000 0.002725176 FALSE TRUE
ENST00000469840 ENSG00000163535 HEK293_OSMI2_6hA HEK293_TMG_6hB SGO2 protein_coding processed_transcript 7.620953 1.718656 16.53414 0.2702612 0.6996098 3.258598 0.3457046 0.1796127 0.3079275 0.0374948 0.08085332 0.7456417 0.1117458 0.1033000 0.01826667 -0.08503333 0.002725176 0.002725176 FALSE FALSE
MSTRG.19736.2 ENSG00000163535 HEK293_OSMI2_6hA HEK293_TMG_6hB SGO2 protein_coding   7.620953 1.718656 16.53414 0.2702612 0.6996098 3.258598 4.1859654 0.8273263 9.4638074 0.4588949 1.14326699 3.5000824 0.3846000 0.4312333 0.57070000 0.13946667 0.910000664 0.002725176 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000163535 E001 0.1614157 0.0326295590 6.874366e-01   2 200510008 200510241 234 + 0.047 0.000 -7.642
ENSG00000163535 E002 0.3040503 0.0274424043 1.000000e+00   2 200510242 200510266 25 + 0.089 0.000 -9.996
ENSG00000163535 E003 0.1779838 0.0338269407 6.881343e-01   2 200525749 200525836 88 + 0.047 0.000 -8.958
ENSG00000163535 E004 2.8992195 0.0089401586 4.278773e-01 0.6493950282 2 200526136 200526158 23 + 0.467 0.283 -1.072
ENSG00000163535 E005 4.2746237 0.0059644633 1.712603e-01 0.3921962888 2 200526159 200526166 8 + 0.596 0.283 -1.683
ENSG00000163535 E006 11.6406023 0.0032541287 2.332530e-01 0.4689054178 2 200526167 200526206 40 + 0.940 0.747 -0.748
ENSG00000163535 E007 17.3256649 0.0021608733 2.133168e-01 0.4456100361 2 200526207 200526252 46 + 1.091 0.921 -0.627
ENSG00000163535 E008 0.3503582 0.0270865566 1.292576e-01   2 200531775 200531901 127 + 0.047 0.283 3.018
ENSG00000163535 E009 0.4986023 0.0389404393 1.000000e+00   2 200532303 200532355 53 + 0.127 0.000 -10.468
ENSG00000163535 E010 30.6564544 0.0013201733 5.086185e-01 0.7069023660 2 200532974 200533108 135 + 1.302 1.362 0.210
ENSG00000163535 E011 0.1614157 0.0326295590 6.874366e-01   2 200533109 200533375 267 + 0.047 0.000 -8.968
ENSG00000163535 E012 25.7572526 0.0017410663 6.006941e-01 0.7706934379 2 200534996 200535045 50 + 1.235 1.287 0.181
ENSG00000163535 E013 34.4052410 0.0030918912 2.140857e-01 0.4464660307 2 200535046 200535140 95 + 1.346 1.455 0.376
ENSG00000163535 E014 25.3147516 0.0013958549 5.285609e-01 0.7210014396 2 200535141 200535171 31 + 1.224 1.287 0.221
ENSG00000163535 E015 38.1888996 0.0012151028 3.265806e-01 0.5657668680 2 200536065 200536142 78 + 1.389 1.469 0.278
ENSG00000163535 E016 45.5711406 0.0010545978 6.116360e-01 0.7779931453 2 200542579 200542664 86 + 1.469 1.508 0.133
ENSG00000163535 E017 0.3088520 0.0253049708 1.305666e-01   2 200542665 200542950 286 + 0.047 0.283 3.014
ENSG00000163535 E018 1.6676880 0.0136681602 8.707392e-01 0.9363817740 2 200543025 200543622 598 + 0.329 0.283 -0.303
ENSG00000163535 E019 76.8651355 0.0004997861 8.962173e-02 0.2631094865 2 200569663 200569892 230 + 1.708 1.597 -0.378
ENSG00000163535 E020 0.3040503 0.0274424043 1.000000e+00   2 200569893 200570142 250 + 0.089 0.000 -9.996
ENSG00000163535 E021 756.6015261 0.0003734653 4.406454e-04 0.0051324916 2 200571050 200573977 2928 + 2.686 2.638 -0.163
ENSG00000163535 E022 84.3433360 0.0007792004 5.184984e-03 0.0355878264 2 200575311 200575461 151 + 1.717 1.866 0.504
ENSG00000163535 E023 47.7577934 0.0050546370 1.990512e-05 0.0003806698 2 200583449 200584096 648 + 1.446 1.749 1.034