ENSG00000162869

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000294952 ENSG00000162869 HEK293_OSMI2_6hA HEK293_TMG_6hB PPP1R21 protein_coding protein_coding 4.966814 2.879058 6.945741 0.5026371 0.2954518 1.267605 2.7815260 1.4990641 3.70173305 0.07666567 0.35394334 1.298439 0.57363333 0.5457667 0.5387333 -0.007033333 1.00000000 0.01892838 FALSE  
ENST00000416913 ENSG00000162869 HEK293_OSMI2_6hA HEK293_TMG_6hB PPP1R21 protein_coding nonsense_mediated_decay 4.966814 2.879058 6.945741 0.5026371 0.2954518 1.267605 1.1464144 0.4736294 1.96692175 0.21955450 0.48284620 2.031282 0.20957500 0.1493000 0.2787000 0.129400000 0.63350436 0.01892838 FALSE  
ENST00000460299 ENSG00000162869 HEK293_OSMI2_6hA HEK293_TMG_6hB PPP1R21 protein_coding retained_intron 4.966814 2.879058 6.945741 0.5026371 0.2954518 1.267605 0.4794772 0.3311830 0.89530958 0.33118301 0.26293196 1.407865 0.09105417 0.0858000 0.1267000 0.040900000 0.61928420 0.01892838 FALSE  
ENST00000476199 ENSG00000162869 HEK293_OSMI2_6hA HEK293_TMG_6hB PPP1R21 protein_coding processed_transcript 4.966814 2.879058 6.945741 0.5026371 0.2954518 1.267605 0.2468851 0.3005738 0.05112537 0.06088606 0.05112537 -2.345093 0.06005417 0.1067333 0.0068000 -0.099933333 0.01892838 0.01892838 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000162869 E001 0.0000000       2 48440598 48440698 101 +      
ENSG00000162869 E002 0.0000000       2 48440766 48440768 3 +      
ENSG00000162869 E003 21.9066934 0.0018584257 0.309674665 0.55012868 2 48440769 48441010 242 + 1.352 1.272 -0.279
ENSG00000162869 E004 0.5227326 0.4379532889 0.322126812   2 48441129 48441470 342 + 0.087 0.296 2.146
ENSG00000162869 E005 31.6700332 0.0089640681 0.366200057 0.60091503 2 48451008 48451076 69 + 1.502 1.429 -0.250
ENSG00000162869 E006 8.4349798 0.0131252531 0.271392135 0.51133443 2 48454556 48454594 39 + 0.995 0.857 -0.520
ENSG00000162869 E007 46.2039682 0.0009936590 0.030946168 0.13019946 2 48454595 48454741 147 + 1.677 1.556 -0.411
ENSG00000162869 E008 0.0000000       2 48458002 48458125 124 +      
ENSG00000162869 E009 34.5221613 0.0053126173 0.939953691 0.97221362 2 48458126 48458227 102 + 1.517 1.521 0.013
ENSG00000162869 E010 38.9414856 0.0012861904 0.144204336 0.35368033 2 48459754 48459918 165 + 1.540 1.626 0.293
ENSG00000162869 E011 24.7721250 0.0014108514 0.474078117 0.68288736 2 48460095 48460153 59 + 1.396 1.344 -0.180
ENSG00000162869 E012 31.4497287 0.0011803095 0.507625450 0.70612036 2 48461138 48461232 95 + 1.494 1.452 -0.145
ENSG00000162869 E013 22.7974250 0.0017584547 0.570382102 0.75050556 2 48464937 48464965 29 + 1.335 1.380 0.156
ENSG00000162869 E014 22.4841132 0.0051638530 0.092994395 0.26929247 2 48464966 48464989 24 + 1.298 1.436 0.476
ENSG00000162869 E015 36.2544173 0.0189478376 0.497008240 0.69893533 2 48465493 48465642 150 + 1.523 1.590 0.227
ENSG00000162869 E016 24.7439420 0.0016142333 0.171412015 0.39240396 2 48471087 48471188 102 + 1.352 1.452 0.345
ENSG00000162869 E017 19.6808686 0.0022965977 0.583054789 0.75904055 2 48471279 48471310 32 + 1.269 1.315 0.160
ENSG00000162869 E018 23.5096835 0.0018165887 0.831102820 0.91441771 2 48471311 48471367 57 + 1.368 1.353 -0.052
ENSG00000162869 E019 2.0088685 0.0135516370 0.964039690 0.98390634 2 48471368 48471450 83 + 0.471 0.464 -0.032
ENSG00000162869 E020 25.4105701 0.0016366705 0.815979529 0.90583100 2 48474683 48474819 137 + 1.396 1.380 -0.054
ENSG00000162869 E021 1.1855836 0.0156905153 0.307175617   2 48479484 48479556 73 + 0.363 0.170 -1.442
ENSG00000162869 E022 19.2977730 0.0019029092 0.772875391 0.88040741 2 48479924 48480016 93 + 1.284 1.261 -0.080
ENSG00000162869 E023 21.5098709 0.0022128704 0.329764058 0.56871979 2 48486631 48486758 128 + 1.340 1.262 -0.273
ENSG00000162869 E024 27.0556092 0.0013417446 0.210204881 0.44208678 2 48491018 48491170 153 + 1.443 1.353 -0.309
ENSG00000162869 E025 26.2092734 0.0013803984 0.023540599 0.10767440 2 48495679 48495771 93 + 1.453 1.284 -0.587
ENSG00000162869 E026 47.9030923 0.0012243425 0.296574380 0.53708808 2 48498493 48498735 243 + 1.680 1.625 -0.188
ENSG00000162869 E027 22.8676254 0.0017117363 0.435437256 0.65531683 2 48505564 48505596 33 + 1.373 1.314 -0.202
ENSG00000162869 E028 0.9989696 0.0153787590 0.078506428   2 48507211 48507268 58 + 0.157 0.465 2.143
ENSG00000162869 E029 33.2459950 0.0011662371 0.693757830 0.83153097 2 48507269 48507385 117 + 1.512 1.487 -0.083
ENSG00000162869 E030 35.6482528 0.0011603606 0.742063706 0.86168347 2 48510015 48510113 99 + 1.529 1.550 0.073
ENSG00000162869 E031 42.1820298 0.0010020586 0.007057249 0.04489542 2 48511340 48511468 129 + 1.553 1.702 0.506
ENSG00000162869 E032 52.9072393 0.0008128033 0.031446558 0.13163406 2 48514715 48515391 677 + 1.667 1.774 0.364