ENSG00000162591

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000294599 ENSG00000162591 HEK293_OSMI2_6hA HEK293_TMG_6hB MEGF6 protein_coding protein_coding 1.706958 2.489139 1.103804 1.020235 0.2367172 -1.165936 0.15980180 0.10638581 0.05658103 0.06754732 0.05658103 -0.8057320 0.10340417 0.067266667 0.03780000 -0.02946667 0.95507944 0.01020583 FALSE TRUE
ENST00000356575 ENSG00000162591 HEK293_OSMI2_6hA HEK293_TMG_6hB MEGF6 protein_coding protein_coding 1.706958 2.489139 1.103804 1.020235 0.2367172 -1.165936 0.66351047 0.90386826 0.32233113 0.32413921 0.16971938 -1.4593648 0.40931250 0.380333333 0.36976667 -0.01056667 0.93917226 0.01020583 FALSE TRUE
ENST00000461795 ENSG00000162591 HEK293_OSMI2_6hA HEK293_TMG_6hB MEGF6 protein_coding retained_intron 1.706958 2.489139 1.103804 1.020235 0.2367172 -1.165936 0.31950385 0.66956683 0.19987808 0.46724493 0.19987808 -1.6950639 0.15532083 0.192700000 0.13350000 -0.05920000 0.81697185 0.01020583 FALSE FALSE
ENST00000475790 ENSG00000162591 HEK293_OSMI2_6hA HEK293_TMG_6hB MEGF6 protein_coding retained_intron 1.706958 2.489139 1.103804 1.020235 0.2367172 -1.165936 0.03925167 0.02153858 0.11146549 0.02153858 0.06568820 1.9453570 0.02682500 0.004833333 0.09060000 0.08576667 0.32894006 0.01020583 FALSE FALSE
ENST00000494257 ENSG00000162591 HEK293_OSMI2_6hA HEK293_TMG_6hB MEGF6 protein_coding retained_intron 1.706958 2.489139 1.103804 1.020235 0.2367172 -1.165936 0.16238514 0.22257216 0.14846892 0.20468194 0.04055257 -0.5534785 0.08698333 0.053366667 0.13486667 0.08150000 0.59201425 0.01020583 TRUE FALSE
MSTRG.166.3 ENSG00000162591 HEK293_OSMI2_6hA HEK293_TMG_6hB MEGF6 protein_coding   1.706958 2.489139 1.103804 1.020235 0.2367172 -1.165936 0.10066190 0.23807961 0.00000000 0.01139874 0.00000000 -4.6327313 0.06267083 0.138033333 0.00000000 -0.13803333 0.06024628 0.01020583 FALSE TRUE
MSTRG.166.4 ENSG00000162591 HEK293_OSMI2_6hA HEK293_TMG_6hB MEGF6 protein_coding   1.706958 2.489139 1.103804 1.020235 0.2367172 -1.165936 0.09173248 0.27028615 0.00000000 0.06654395 0.00000000 -4.8088285 0.05040833 0.129300000 0.00000000 -0.12930000 0.01020583 0.01020583 FALSE TRUE
MSTRG.166.5 ENSG00000162591 HEK293_OSMI2_6hA HEK293_TMG_6hB MEGF6 protein_coding   1.706958 2.489139 1.103804 1.020235 0.2367172 -1.165936 0.05262098 0.00000000 0.17450771 0.00000000 0.17450771 4.2056092 0.03938750 0.000000000 0.15370000 0.15370000 0.79359078 0.01020583 FALSE TRUE
MSTRG.166.7 ENSG00000162591 HEK293_OSMI2_6hA HEK293_TMG_6hB MEGF6 protein_coding   1.706958 2.489139 1.103804 1.020235 0.2367172 -1.165936 0.09482731 0.00000000 0.09057126 0.00000000 0.09057126 3.3301462 0.05236250 0.000000000 0.07976667 0.07976667 0.76962222 0.01020583 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000162591 E001 35.2213347 0.373311796 0.65791451 0.80861100 1 3487951 3489919 1969 - 1.436 1.483 0.163
ENSG00000162591 E002 19.5889716 0.048286674 0.17208242 0.39330299 1 3489920 3490309 390 - 1.080 1.268 0.667
ENSG00000162591 E003 5.6588910 0.005224834 0.55145858 0.73731725 1 3490310 3490311 2 - 0.673 0.770 0.399
ENSG00000162591 E004 13.1635404 0.037878487 0.47742683 0.68532920 1 3490312 3490440 129 - 1.007 1.087 0.289
ENSG00000162591 E005 15.2202214 0.087754440 0.31407569 0.55431477 1 3490441 3490561 121 - 1.027 1.154 0.457
ENSG00000162591 E006 8.7795130 0.022377593 0.24309976 0.48013736 1 3490562 3490589 28 - 0.772 0.950 0.687
ENSG00000162591 E007 8.1899036 0.036993330 0.22019661 0.45376657 1 3490912 3490924 13 - 0.724 0.931 0.810
ENSG00000162591 E008 8.6065184 0.008599746 0.13247994 0.33572300 1 3490925 3490959 35 - 0.726 0.950 0.874
ENSG00000162591 E009 7.8742272 0.004062569 0.62540097 0.78756206 1 3492639 3492727 89 - 0.817 0.889 0.277
ENSG00000162591 E010 4.4437284 0.009070765 0.19049218 0.41757616 1 3492728 3492767 40 - 0.817 0.622 -0.802
ENSG00000162591 E011 4.1791542 0.303710597 0.11192432 0.30230335 1 3492768 3493486 719 - 0.820 0.577 -1.014
ENSG00000162591 E012 6.2906470 0.005264888 0.31776652 0.55773928 1 3493771 3493797 27 - 0.892 0.757 -0.528
ENSG00000162591 E013 11.8521366 0.002843772 0.15398544 0.36801302 1 3493798 3493899 102 - 1.145 0.997 -0.540
ENSG00000162591 E014 12.8389595 0.002594483 0.26518383 0.50454657 1 3493996 3494124 129 - 1.145 1.032 -0.408
ENSG00000162591 E015 2.3718776 0.258986132 0.99710065 1.00000000 1 3494125 3494244 120 - 0.449 0.480 0.158
ENSG00000162591 E016 9.2325499 0.015332532 0.87668718 0.93952978 1 3494371 3494499 129 - 0.925 0.940 0.059
ENSG00000162591 E017 0.7007164 0.403766621 0.28098131   1 3494500 3494612 113 - 0.355 0.128 -1.886
ENSG00000162591 E018 8.3677701 0.004646101 0.95528413 0.97955945 1 3494613 3494741 129 - 0.892 0.889 -0.013
ENSG00000162591 E019 7.0949353 0.004870926 0.91910762 0.96178753 1 3495890 3496018 129 - 0.817 0.839 0.084
ENSG00000162591 E020 8.8576112 0.015317250 0.82554919 0.91125928 1 3496655 3496783 129 - 0.892 0.917 0.095
ENSG00000162591 E021 0.2027342 0.158462536 1.00000000   1 3496988 3497119 132 - 0.000 0.068 7.814
ENSG00000162591 E022 7.3110909 0.064258074 0.20775418 0.43910719 1 3497233 3497361 129 - 0.673 0.884 0.843
ENSG00000162591 E023 3.5382819 0.007153116 0.13260791 0.33589192 1 3497491 3497621 131 - 0.349 0.640 1.446
ENSG00000162591 E024 8.0247339 0.004825825 0.36174307 0.59707929 1 3498371 3498499 129 - 0.774 0.907 0.514
ENSG00000162591 E025 12.4697998 0.003162503 0.96515496 0.98449067 1 3498698 3498826 129 - 1.037 1.046 0.032
ENSG00000162591 E026 11.9298446 0.003372928 0.13022424 0.33216285 1 3499138 3499266 129 - 1.146 0.990 -0.566
ENSG00000162591 E027 8.5382230 0.003631942 0.44341068 0.66098119 1 3499588 3499716 129 - 0.817 0.925 0.415
ENSG00000162591 E028 8.3254554 0.004882935 0.68428105 0.82546624 1 3499796 3499924 129 - 0.925 0.879 -0.175
ENSG00000162591 E029 10.0701641 0.004079426 0.56330936 0.74560132 1 3500633 3500764 132 - 1.012 0.950 -0.229
ENSG00000162591 E030 8.3901826 0.004934147 0.50260600 0.70258050 1 3500966 3501094 129 - 0.817 0.916 0.380
ENSG00000162591 E031 8.8789710 0.004306570 0.22290180 0.45686251 1 3501177 3501308 132 - 0.774 0.950 0.678
ENSG00000162591 E032 7.3498702 0.007502556 0.01480613 0.07734541 1 3501796 3501921 126 - 1.061 0.757 -1.155
ENSG00000162591 E033 11.6503528 0.035144414 0.96218016 0.98311244 1 3505208 3505342 135 - 1.012 1.017 0.019
ENSG00000162591 E034 12.0361640 0.003264924 0.47576538 0.68405062 1 3505422 3505556 135 - 0.956 1.046 0.334
ENSG00000162591 E035 10.0591715 0.048197954 0.97866135 0.99111693 1 3506108 3506236 129 - 0.957 0.957 -0.001
ENSG00000162591 E036 8.1957126 0.021348974 0.22811728 0.46309498 1 3507795 3507923 129 - 1.012 0.839 -0.652
ENSG00000162591 E037 9.0968432 0.057000822 0.14337611 0.35250661 1 3508558 3508689 132 - 1.086 0.880 -0.764
ENSG00000162591 E038 12.0907878 0.006866514 0.08009774 0.24501835 1 3509075 3509245 171 - 1.182 0.996 -0.676
ENSG00000162591 E039 5.7536748 0.005438531 0.48881602 0.69318506 1 3509870 3509992 123 - 0.673 0.782 0.446
ENSG00000162591 E040 6.3028460 0.019644988 0.23520711 0.47113830 1 3510783 3510902 120 - 0.925 0.744 -0.704
ENSG00000162591 E041 6.9988603 0.028333970 0.88389012 0.94328455 1 3511550 3511687 138 - 0.854 0.819 -0.135
ENSG00000162591 E042 7.4861735 0.005571733 0.89509026 0.94927192 1 3512006 3512128 123 - 0.856 0.840 -0.064
ENSG00000162591 E043 7.1885405 0.009479524 0.54199488 0.73050156 1 3514550 3514672 123 - 0.892 0.807 -0.328
ENSG00000162591 E044 9.5340969 0.025251010 0.42215438 0.64526662 1 3515402 3515527 126 - 0.818 0.963 0.554
ENSG00000162591 E045 7.1552225 0.005562744 0.92755732 0.96584125 1 3524124 3524246 123 - 0.817 0.839 0.085
ENSG00000162591 E046 0.3336024 0.032584248 0.99366872   1 3531080 3531448 369 - 0.000 0.126 8.797
ENSG00000162591 E047 0.0000000       1 3546998 3547123 126 -      
ENSG00000162591 E048 6.5988739 0.027426060 0.20514355 0.43608122 1 3579825 3579929 105 - 0.926 0.760 -0.645
ENSG00000162591 E049 9.2049480 0.003497380 0.72539073 0.85139717 1 3595338 3595447 110 - 0.956 0.916 -0.148
ENSG00000162591 E050 6.3943936 0.005382166 0.17500796 0.39716665 1 3602466 3602600 135 - 0.612 0.828 0.890
ENSG00000162591 E051 4.9676629 0.050399373 0.11088666 0.30042069 1 3611138 3611508 371 - 0.456 0.755 1.334