ENSG00000162408

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000377705 ENSG00000162408 HEK293_OSMI2_6hA HEK293_TMG_6hB NOL9 protein_coding protein_coding 18.29902 14.88229 20.64818 1.538163 0.9781163 0.4721483 10.581171 12.3165847 7.039931 1.80557696 0.2793434 -0.8060921 0.60335000 0.81966667 0.3412000 -0.4784667 8.851571e-09 8.851571e-09 FALSE TRUE
MSTRG.201.4 ENSG00000162408 HEK293_OSMI2_6hA HEK293_TMG_6hB NOL9 protein_coding   18.29902 14.88229 20.64818 1.538163 0.9781163 0.4721483 3.980808 0.2006978 5.889697 0.20069777 0.7216897 4.8073940 0.19844167 0.01673333 0.2866333 0.2699000 3.514285e-02 8.851571e-09 FALSE TRUE
MSTRG.201.5 ENSG00000162408 HEK293_OSMI2_6hA HEK293_TMG_6hB NOL9 protein_coding   18.29902 14.88229 20.64818 1.538163 0.9781163 0.4721483 1.815741 0.9231902 4.799590 0.06062963 1.3609663 2.3656708 0.09334583 0.06263333 0.2296333 0.1670000 1.593573e-02 8.851571e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000162408 E001 537.279122 0.0330424387 2.683691e-03 2.147907e-02 1 6521347 6523258 1912 - 2.474 2.863 1.297
ENSG00000162408 E002 268.009934 1.1495066245 3.306680e-01 5.696172e-01 1 6523259 6524275 1017 - 2.204 2.551 1.158
ENSG00000162408 E003 75.190331 0.4438318562 1.986827e-01 4.280211e-01 1 6524276 6524409 134 - 1.671 1.998 1.103
ENSG00000162408 E004 104.495435 0.5627657076 2.180573e-01 4.511097e-01 1 6524410 6524805 396 - 1.809 2.141 1.115
ENSG00000162408 E005 113.692412 0.5987509029 2.370549e-01 4.731541e-01 1 6524806 6525571 766 - 1.854 2.173 1.072
ENSG00000162408 E006 111.876962 0.6733754474 3.935142e-01 6.231665e-01 1 6525572 6525718 147 - 1.937 2.125 0.631
ENSG00000162408 E007 216.573671 1.2272812133 6.105919e-01 7.773416e-01 1 6525719 6526003 285 - 2.284 2.373 0.297
ENSG00000162408 E008 149.851051 0.0006248832 1.002516e-04 1.498023e-03 1 6526696 6526829 134 - 2.182 2.173 -0.031
ENSG00000162408 E009 168.041290 0.0245915639 2.499853e-02 1.122201e-01 1 6528994 6529171 178 - 2.241 2.213 -0.093
ENSG00000162408 E010 79.318217 0.0134774866 7.679551e-02 2.383734e-01 1 6531968 6531994 27 - 1.897 1.909 0.042
ENSG00000162408 E011 97.961741 0.0005368520 1.290180e-01 3.302335e-01 1 6531995 6532079 85 - 1.967 2.020 0.178
ENSG00000162408 E012 4.611842 0.0068059454 3.531255e-03 2.652784e-02 1 6532229 6532462 234 - 0.905 0.596 -1.257
ENSG00000162408 E013 177.670096 0.2973061586 5.809800e-01 7.577911e-01 1 6532463 6532760 298 - 2.210 2.280 0.236
ENSG00000162408 E014 139.653186 1.0729257738 6.564309e-01 8.076176e-01 1 6533280 6533441 162 - 2.122 2.165 0.145
ENSG00000162408 E015 77.449675 0.0071483798 2.712076e-02 1.186493e-01 1 6541830 6541927 98 - 1.911 1.881 -0.098
ENSG00000162408 E016 99.760782 0.0055152577 3.591595e-06 8.662626e-05 1 6544826 6544919 94 - 2.058 1.952 -0.355
ENSG00000162408 E017 51.062466 0.0398195658 3.617507e-02 1.446960e-01 1 6544920 6544922 3 - 1.757 1.676 -0.272
ENSG00000162408 E018 3.342845 0.0382115733 7.042807e-01 8.383255e-01 1 6544923 6545017 95 - 0.621 0.655 0.148
ENSG00000162408 E019 126.229733 0.0415629012 1.627053e-03 1.451028e-02 1 6545045 6545180 136 - 2.187 2.017 -0.571
ENSG00000162408 E020 1.855492 0.2166329011 9.912583e-01 9.973898e-01 1 6549256 6549361 106 - 0.384 0.492 0.568
ENSG00000162408 E021 4.329856 0.1846562925 8.795038e-01 9.410459e-01 1 6549362 6549570 209 - 0.657 0.767 0.454
ENSG00000162408 E022 135.030574 0.0581940328 2.308041e-02 1.062074e-01 1 6549571 6549698 128 - 2.192 2.075 -0.391
ENSG00000162408 E023 140.203503 0.0674723026 2.079105e-02 9.864494e-02 1 6550396 6550615 220 - 2.219 2.079 -0.471
ENSG00000162408 E024 69.547998 0.0728142334 5.851621e-01 7.604143e-01 1 6554107 6554514 408 - 1.805 1.877 0.245