ENSG00000161547

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000358156 ENSG00000161547 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF2 protein_coding protein_coding 416.6683 309.4587 351.0563 24.68887 7.932092 0.1819497 2.027008 16.10513 0.00000 9.355055 0.000000 -10.6542000 0.00668750 0.05330000 0.0000000 -0.05330000 0.2611548012 1.593301e-29 FALSE FALSE
ENST00000359995 ENSG00000161547 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF2 protein_coding protein_coding 416.6683 309.4587 351.0563 24.68887 7.932092 0.1819497 297.918689 205.47684 174.99588 32.265832 3.815400 -0.2316427 0.68687083 0.65623333 0.4987000 -0.15753333 0.3311072223 1.593301e-29 FALSE TRUE
ENST00000392485 ENSG00000161547 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF2 protein_coding protein_coding 416.6683 309.4587 351.0563 24.68887 7.932092 0.1819497 28.977517 12.80470 41.29172 1.288242 1.145229 1.6884020 0.07253750 0.04266667 0.1178667 0.07520000 0.0011494153 1.593301e-29 FALSE TRUE
ENST00000585202 ENSG00000161547 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF2 protein_coding nonsense_mediated_decay 416.6683 309.4587 351.0563 24.68887 7.932092 0.1819497 31.334458 39.95759 22.48983 4.108257 2.302437 -0.8289167 0.08153750 0.13243333 0.0640000 -0.06843333 0.1167136970 1.593301e-29 TRUE TRUE
ENST00000586778 ENSG00000161547 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF2 protein_coding retained_intron 416.6683 309.4587 351.0563 24.68887 7.932092 0.1819497 20.501118 11.64827 45.54469 1.352648 2.719357 1.9662455 0.05615833 0.03883333 0.1296333 0.09080000 0.0001262185 1.593301e-29   FALSE
ENST00000592676 ENSG00000161547 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF2 protein_coding retained_intron 416.6683 309.4587 351.0563 24.68887 7.932092 0.1819497 19.888143 10.48217 50.53881 2.254044 3.283228 2.2683635 0.05518333 0.03546667 0.1438333 0.10836667 0.0015977324 1.593301e-29   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000161547 E001 10.056976 0.014422452 8.294875e-04 8.522874e-03 17 76734115 76734122 8 - 0.736 1.177 1.656
ENSG00000161547 E002 34.220156 0.086596695 5.231652e-02 1.850603e-01 17 76734123 76734146 24 - 1.314 1.655 1.172
ENSG00000161547 E003 54.617623 0.086757682 2.809948e-01 5.212566e-01 17 76734147 76734162 16 - 1.606 1.816 0.713
ENSG00000161547 E004 590.484533 0.033998019 1.990860e-01 4.285690e-01 17 76734163 76734251 89 - 2.806 2.735 -0.236
ENSG00000161547 E005 955.717156 0.031082177 5.703167e-02 1.961187e-01 17 76734252 76734302 51 - 3.041 2.920 -0.403
ENSG00000161547 E006 2741.928599 0.031911542 3.124364e-02 1.310484e-01 17 76734303 76734562 260 - 3.503 3.374 -0.429
ENSG00000161547 E007 893.609886 0.030747074 7.303003e-02 2.308972e-01 17 76734563 76734576 14 - 3.008 2.895 -0.374
ENSG00000161547 E008 3490.762659 0.039828059 2.229123e-01 4.568625e-01 17 76734577 76735060 484 - 3.569 3.513 -0.186
ENSG00000161547 E009 2551.123815 0.000255480 9.926761e-01 9.979991e-01 17 76735061 76735136 76 - 3.391 3.411 0.065
ENSG00000161547 E010 1956.635733 0.006073589 6.139450e-01 7.794997e-01 17 76735137 76735158 22 - 3.270 3.299 0.098
ENSG00000161547 E011 951.311867 0.069549112 3.935195e-06 9.371570e-05 17 76735159 76735617 459 - 3.205 2.657 -1.822
ENSG00000161547 E012 431.634271 0.040728247 5.371025e-09 2.677991e-07 17 76735618 76735771 154 - 2.863 2.312 -1.833
ENSG00000161547 E013 402.814016 0.038054012 8.484461e-05 1.303943e-03 17 76735772 76735875 104 - 2.775 2.406 -1.231
ENSG00000161547 E014 529.442725 0.377201802 4.984152e-01 6.997261e-01 17 76735876 76736153 278 - 2.909 2.495 -1.380
ENSG00000161547 E015 3118.236276 0.295079452 2.271370e-01 4.619199e-01 17 76736154 76736379 226 - 3.391 3.550 0.529
ENSG00000161547 E016 879.172174 1.643827440 4.068688e-01 6.336612e-01 17 76736380 76736404 25 - 2.759 3.037 0.927
ENSG00000161547 E017 797.826142 1.571494741 3.394795e-01 5.775266e-01 17 76736405 76736443 39 - 2.661 3.015 1.178
ENSG00000161547 E018 478.355111 1.331141647 2.306944e-01 4.659311e-01 17 76736444 76736449 6 - 2.351 2.818 1.556
ENSG00000161547 E019 522.625024 1.351103603 2.236492e-01 4.577881e-01 17 76736450 76736464 15 - 2.377 2.860 1.609
ENSG00000161547 E020 5.385384 0.167281022 7.152702e-01 8.451158e-01 17 76736465 76736555 91 - 0.780 0.840 0.233
ENSG00000161547 E021 611.546392 1.351243870 1.786814e-01 4.022138e-01 17 76736799 76736834 36 - 2.377 2.943 1.884
ENSG00000161547 E022 2643.855866 1.970268583 4.115165e-01 6.371987e-01 17 76736835 76737333 499 - 3.212 3.524 1.035