ENSG00000161016

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262584 ENSG00000161016 HEK293_OSMI2_6hA HEK293_TMG_6hB RPL8 protein_coding protein_coding 6952.079 10519.72 3059.749 2695.409 146.0477 -1.781608 1121.8546 1809.317 524.6843 459.3311 18.89685 -1.785905 0.1669083 0.1732667 0.1719000 -0.0013666667 1.000000000 0.006221728 FALSE  
ENST00000526668 ENSG00000161016 HEK293_OSMI2_6hA HEK293_TMG_6hB RPL8 protein_coding retained_intron 6952.079 10519.72 3059.749 2695.409 146.0477 -1.781608 3338.8759 5046.273 1601.5761 1395.3223 107.58496 -1.655720 0.4876458 0.4710333 0.5224667 0.0514333333 0.673519961 0.006221728 FALSE  
ENST00000531975 ENSG00000161016 HEK293_OSMI2_6hA HEK293_TMG_6hB RPL8 protein_coding nonsense_mediated_decay 6952.079 10519.72 3059.749 2695.409 146.0477 -1.781608 1500.9844 1999.344 599.1937 462.7505 14.85796 -1.738416 0.2124750 0.1960667 0.1965333 0.0004666667 1.000000000 0.006221728 FALSE  
ENST00000533397 ENSG00000161016 HEK293_OSMI2_6hA HEK293_TMG_6hB RPL8 protein_coding protein_coding 6952.079 10519.72 3059.749 2695.409 146.0477 -1.781608 860.2367 1488.877 277.2033 373.1128 28.74910 -2.425165 0.1135667 0.1421000 0.0903000 -0.0518000000 0.006221728 0.006221728 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000161016 E001 5.279416e+00 2.175758e-02 4.807392e-01 6.876058e-01 8 144789679 144789709 31 - 0.801 0.678 -0.497
ENSG00000161016 E002 3.698899e+00 5.780942e-02 4.725958e-01 6.819294e-01 8 144789710 144789717 8 - 0.698 0.548 -0.655
ENSG00000161016 E003 4.995694e+00 5.630868e-03 6.247780e-01 7.872033e-01 8 144789718 144789753 36 - 0.752 0.677 -0.308
ENSG00000161016 E004 5.852769e+00 4.983921e-03 9.983968e-01 1.000000e+00 8 144789754 144789763 10 - 0.752 0.755 0.012
ENSG00000161016 E005 5.644858e+00 5.536141e-03 7.400258e-01 8.604585e-01 8 144789764 144789764 1 - 0.698 0.755 0.234
ENSG00000161016 E006 1.973881e+01 1.430724e-02 1.603439e-02 8.193688e-02 8 144789765 144789765 1 - 0.954 1.271 1.143
ENSG00000161016 E007 2.170966e+01 9.319910e-03 7.749790e-02 2.398609e-01 8 144789766 144789766 1 - 1.090 1.297 0.737
ENSG00000161016 E008 2.921729e+01 1.712357e-02 3.039693e-01 5.445365e-01 8 144789767 144789767 1 - 1.278 1.407 0.450
ENSG00000161016 E009 4.002540e+01 1.514225e-02 6.351429e-01 7.936622e-01 8 144789768 144789768 1 - 1.534 1.511 -0.077
ENSG00000161016 E010 2.093752e+02 6.337328e-03 8.839939e-03 5.310687e-02 8 144789769 144789769 1 - 2.097 2.244 0.493
ENSG00000161016 E011 8.286263e+03 4.434280e-04 2.498025e-01 4.876531e-01 8 144789770 144789801 32 - 3.805 3.813 0.029
ENSG00000161016 E012 9.387033e+03 5.141702e-04 8.372786e-01 9.179357e-01 8 144789802 144789808 7 - 3.867 3.865 -0.007
ENSG00000161016 E013 3.864741e+04 4.909583e-04 8.225823e-01 9.094833e-01 8 144789809 144789962 154 - 4.485 4.478 -0.021
ENSG00000161016 E014 5.488151e+04 1.483634e-04 2.244441e-01 4.587452e-01 8 144790355 144790470 116 - 4.642 4.630 -0.039
ENSG00000161016 E015 1.960817e+02 3.140227e-02 2.063220e-01 4.373490e-01 8 144790471 144790677 207 - 2.303 2.156 -0.492
ENSG00000161016 E016 6.656533e+01 1.202942e-01 9.514173e-02 2.732405e-01 8 144790678 144790763 86 - 1.959 1.645 -1.060
ENSG00000161016 E017 6.092478e+01 1.554421e-01 3.048552e-02 1.287862e-01 8 144790764 144790872 109 - 2.003 1.563 -1.487
ENSG00000161016 E018 2.123309e+02 2.218975e-02 1.775540e-01 4.005201e-01 8 144791113 144791276 164 - 2.328 2.193 -0.450
ENSG00000161016 E019 3.421472e+04 8.701209e-05 5.907634e-02 2.006962e-01 8 144791277 144791307 31 - 4.440 4.424 -0.052
ENSG00000161016 E020 2.257135e+04 1.265365e-04 3.038773e-14 4.148340e-12 8 144791308 144791310 3 - 4.288 4.237 -0.170
ENSG00000161016 E021 4.201515e+04 4.547454e-05 5.605749e-17 1.113433e-14 8 144791311 144791370 60 - 4.547 4.510 -0.121
ENSG00000161016 E022 5.213665e+04 1.104625e-04 6.766179e-03 4.354617e-02 8 144791371 144791495 125 - 4.625 4.606 -0.063
ENSG00000161016 E023 1.084598e+02 1.062787e-02 6.107639e-02 2.051905e-01 8 144791496 144791506 11 - 2.040 1.902 -0.462
ENSG00000161016 E024 6.911156e+01 1.253016e-01 2.491538e-02 1.119603e-01 8 144791507 144791628 122 - 2.040 1.627 -1.392
ENSG00000161016 E025 5.907210e+01 1.317909e-01 5.865527e-02 1.997522e-01 8 144791629 144791677 49 - 1.938 1.580 -1.210
ENSG00000161016 E026 5.440486e+01 9.821630e-02 1.444046e-02 7.603644e-02 8 144791678 144791772 95 - 1.941 1.524 -1.414
ENSG00000161016 E027 3.496054e+04 2.081789e-04 7.190089e-01 8.475695e-01 8 144791773 144791849 77 - 4.443 4.435 -0.028
ENSG00000161016 E028 2.375018e+04 1.438931e-04 5.787846e-04 6.388751e-03 8 144791850 144791872 23 - 4.255 4.272 0.055
ENSG00000161016 E029 2.697215e+04 1.159488e-05 7.049150e-61 4.275385e-57 8 144791873 144791914 42 - 4.287 4.337 0.169
ENSG00000161016 E030 9.653667e+01 9.331173e-03 5.245581e-01 7.181734e-01 8 144791915 144791930 16 - 1.928 1.871 -0.194
ENSG00000161016 E031 7.754906e+01 9.593545e-03 1.627444e-02 8.279732e-02 8 144791931 144791991 61 - 1.928 1.751 -0.596
ENSG00000161016 E032 3.858562e+04 9.904726e-04 2.168846e-02 1.015579e-01 8 144791992 144792140 149 - 4.460 4.483 0.078
ENSG00000161016 E033 5.241512e+02 2.697195e-03 1.932513e-01 4.211485e-01 8 144792141 144792244 104 - 2.583 2.620 0.123
ENSG00000161016 E034 7.811272e+02 1.155828e-04 5.327782e-18 1.234352e-15 8 144792245 144792265 21 - 2.661 2.818 0.523
ENSG00000161016 E035 9.586460e+02 9.888163e-05 1.167693e-21 4.157255e-19 8 144792266 144792365 100 - 2.750 2.908 0.524
ENSG00000161016 E036 1.788003e+04 1.031531e-03 3.159823e-02 1.320563e-01 8 144792366 144793117 752 - 4.125 4.149 0.082
ENSG00000161016 E037 4.812263e-01 2.149749e-02 5.348168e-01   8 144797400 144797443 44 - 0.221 0.118 -1.086