ENSG00000160563

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000292035 ENSG00000160563 HEK293_OSMI2_6hA HEK293_TMG_6hB MED27 protein_coding protein_coding 59.06741 54.85608 45.26952 8.597007 3.081939 -0.2770559 51.999023 51.51508 35.583684 7.732326 1.294572 -0.5336536 0.86757917 0.941 0.7918333 -0.1491667 7.684972e-03 9.920248e-19 FALSE TRUE
ENST00000650776 ENSG00000160563 HEK293_OSMI2_6hA HEK293_TMG_6hB MED27 protein_coding retained_intron 59.06741 54.85608 45.26952 8.597007 3.081939 -0.2770559 4.139559 0.00000 8.281613 0.000000 2.550180 9.6955090 0.08417917 0.000 0.1776333 0.1776333 9.920248e-19 9.920248e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000160563 E001 0.1614157 0.0318804754 2.999199e-01   9 131852928 131853144 217 - 0.150 0.000 -11.699
ENSG00000160563 E002 0.0000000       9 131853558 131853619 62 -      
ENSG00000160563 E003 1.0423134 0.0158157273 3.477596e-02   9 131860107 131860111 5 - 0.000 0.400 12.820
ENSG00000160563 E004 146.3318044 0.0012862293 7.893846e-03 0.048834526 9 131860112 131860237 126 - 2.090 2.184 0.316
ENSG00000160563 E005 560.2361369 0.0015516476 8.766908e-05 0.001338508 9 131860238 131860672 435 - 2.674 2.764 0.300
ENSG00000160563 E006 232.5271507 0.0002331582 5.049438e-03 0.034892198 9 131863063 131863140 78 - 2.302 2.373 0.238
ENSG00000160563 E007 171.1828912 0.0002548206 3.062266e-01 0.546901500 9 131884058 131884099 42 - 2.230 2.205 -0.083
ENSG00000160563 E008 1.2154955 0.0152176250 6.171535e-01   9 131887944 131888033 90 - 0.262 0.354 0.610
ENSG00000160563 E009 308.6729231 0.0009308482 2.245600e-01 0.458877159 9 131893885 131893992 108 - 2.484 2.461 -0.076
ENSG00000160563 E010 0.5474829 0.0649218382 1.991519e-01   9 131937924 131939380 1457 - 0.000 0.246 11.829
ENSG00000160563 E011 323.1766988 0.0021405809 1.845111e-01 0.409828213 9 131939381 131939474 94 - 2.508 2.481 -0.093
ENSG00000160563 E012 0.0000000       9 132014282 132014336 55 -      
ENSG00000160563 E013 376.7821595 0.0007390595 5.264920e-02 0.185861318 9 132014337 132014467 131 - 2.578 2.544 -0.112
ENSG00000160563 E014 0.0000000       9 132031886 132032169 284 -      
ENSG00000160563 E015 2.3933767 0.0089625171 7.330506e-01 0.856017075 9 132073351 132073742 392 - 0.487 0.546 0.283
ENSG00000160563 E016 324.5910766 0.0001680249 1.764541e-02 0.087797561 9 132077442 132077586 145 - 2.519 2.476 -0.143
ENSG00000160563 E017 290.2319700 0.0003359593 3.170589e-03 0.024435626 9 132079642 132079867 226 - 2.481 2.422 -0.198