ENSG00000160460

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392023 ENSG00000160460 HEK293_OSMI2_6hA HEK293_TMG_6hB SPTBN4 protein_coding protein_coding 2.502977 3.69109 0.4764637 0.3653246 0.03345245 -2.927546 0.64650360 0.90303574 0.12709913 0.10675735 0.004863441 -2.735451935 0.25103750 0.246866667 0.27050000 0.02363333 0.96329902 0.02556845 FALSE TRUE
ENST00000593816 ENSG00000160460 HEK293_OSMI2_6hA HEK293_TMG_6hB SPTBN4 protein_coding processed_transcript 2.502977 3.69109 0.4764637 0.3653246 0.03345245 -2.927546 0.10262562 0.11352748 0.11385584 0.09092047 0.059798081 0.003829868 0.06645833 0.035266667 0.24700000 0.21173333 0.44507407 0.02556845   FALSE
ENST00000595690 ENSG00000160460 HEK293_OSMI2_6hA HEK293_TMG_6hB SPTBN4 protein_coding retained_intron 2.502977 3.69109 0.4764637 0.3653246 0.03345245 -2.927546 0.09502192 0.01663665 0.06079669 0.01663665 0.030792761 1.410269406 0.05062500 0.004766667 0.13573333 0.13096667 0.09514881 0.02556845   FALSE
ENST00000596900 ENSG00000160460 HEK293_OSMI2_6hA HEK293_TMG_6hB SPTBN4 protein_coding retained_intron 2.502977 3.69109 0.4764637 0.3653246 0.03345245 -2.927546 0.15655488 0.19195128 0.04820583 0.06842713 0.032286839 -1.794771826 0.07237917 0.049733333 0.09403333 0.04430000 0.86522579 0.02556845 FALSE TRUE
ENST00000597389 ENSG00000160460 HEK293_OSMI2_6hA HEK293_TMG_6hB SPTBN4 protein_coding nonsense_mediated_decay 2.502977 3.69109 0.4764637 0.3653246 0.03345245 -2.927546 0.14201213 0.08698731 0.04048218 0.04425604 0.020488930 -0.942021688 0.06462500 0.022600000 0.07950000 0.05690000 0.57735957 0.02556845 FALSE TRUE
ENST00000599926 ENSG00000160460 HEK293_OSMI2_6hA HEK293_TMG_6hB SPTBN4 protein_coding processed_transcript 2.502977 3.69109 0.4764637 0.3653246 0.03345245 -2.927546 0.75093324 1.57440243 0.03125738 0.22286736 0.031257377 -5.263142905 0.27372500 0.428666667 0.06063333 -0.36803333 0.02556845 0.02556845   FALSE
MSTRG.17165.6 ENSG00000160460 HEK293_OSMI2_6hA HEK293_TMG_6hB SPTBN4 protein_coding   2.502977 3.69109 0.4764637 0.3653246 0.03345245 -2.927546 0.49835910 0.57685371 0.04222420 0.34013999 0.042224202 -3.490210464 0.17322917 0.147200000 0.08190000 -0.06530000 0.92037515 0.02556845 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000160460 E001 0.3751086 0.027690937 1.00000000   19 40466241 40466311 71 + 0.000 0.100 9.140
ENSG00000160460 E002 0.0000000       19 40467001 40467305 305 +      
ENSG00000160460 E003 0.3032425 0.027442404 1.00000000   19 40472607 40472790 184 + 0.000 0.099 11.172
ENSG00000160460 E004 0.6592102 0.023733208 0.06906608   19 40487697 40487848 152 + 0.419 0.099 -2.659
ENSG00000160460 E005 0.1723744 0.032318203 0.79243781   19 40490075 40490248 174 + 0.000 0.053 10.334
ENSG00000160460 E006 0.1426347 0.034138420 0.04897175   19 40492963 40493054 92 + 0.257 0.000 -14.761
ENSG00000160460 E007 0.3150090 0.028279462 0.16096613   19 40494897 40494977 81 + 0.257 0.053 -2.653
ENSG00000160460 E008 0.0000000       19 40497429 40497488 60 +      
ENSG00000160460 E009 0.3150090 0.028279462 0.16096613   19 40497489 40497604 116 + 0.257 0.053 -2.653
ENSG00000160460 E010 0.4873834 0.271425103 0.34178144   19 40501921 40502033 113 + 0.256 0.100 -1.624
ENSG00000160460 E011 0.3447487 0.416502069 1.00000000   19 40502128 40502315 188 + 0.000 0.101 11.066
ENSG00000160460 E012 0.0000000       19 40502390 40502507 118 +      
ENSG00000160460 E013 0.1723744 0.032318203 0.79243781   19 40502775 40502933 159 + 0.000 0.053 10.334
ENSG00000160460 E014 0.4756169 0.026521228 1.00000000   19 40503830 40504132 303 + 0.000 0.142 11.640
ENSG00000160460 E015 0.1308682 0.031319897 0.78907337   19 40506236 40506386 151 + 0.000 0.052 10.332
ENSG00000160460 E016 0.1308682 0.031319897 0.78907337   19 40512606 40512613 8 + 0.000 0.052 10.332
ENSG00000160460 E017 0.4341107 0.024190347 1.00000000   19 40512614 40513554 941 + 0.000 0.141 11.638
ENSG00000160460 E018 0.0000000       19 40515311 40515448 138 +      
ENSG00000160460 E019 0.1723744 0.032318203 0.79243781   19 40519401 40520057 657 + 0.000 0.053 10.334
ENSG00000160460 E020 0.3751086 0.027690937 1.00000000   19 40520058 40520151 94 + 0.000 0.100 11.176
ENSG00000160460 E021 4.2154593 0.009492523 0.92951335 0.96677704 19 40523437 40523639 203 + 0.535 0.584 0.223
ENSG00000160460 E022 0.0000000       19 40524544 40524629 86 +      
ENSG00000160460 E023 5.4350164 0.072366163 0.64271018 0.79866364 19 40529041 40529131 91 + 0.705 0.648 -0.238
ENSG00000160460 E024 0.3751086 0.027690937 1.00000000   19 40530488 40530525 38 + 0.000 0.100 11.176
ENSG00000160460 E025 1.2674129 0.133116622 0.31805992   19 40530526 40530590 65 + 0.421 0.219 -1.317
ENSG00000160460 E026 0.0000000       19 40530766 40530856 91 +      
ENSG00000160460 E027 8.6442516 0.012919230 0.82237622 0.90934092 19 40532625 40532771 147 + 0.825 0.825 0.003
ENSG00000160460 E028 11.1731577 0.004128703 0.32479474 0.56402418 19 40534080 40534343 264 + 0.768 0.953 0.714
ENSG00000160460 E029 0.3336024 0.027442404 1.00000000   19 40534344 40534856 513 + 0.000 0.099 11.173
ENSG00000160460 E030 1.5307943 0.475235702 0.46863527 0.67911065 19 40538613 40538735 123 + 0.000 0.329 12.693
ENSG00000160460 E031 6.8027472 0.004996356 0.01505561 0.07829219 19 40549189 40549413 225 + 0.258 0.805 2.734
ENSG00000160460 E032 3.1123584 0.009132097 0.04037920 0.15572070 19 40550238 40550327 90 + 0.000 0.552 13.680
ENSG00000160460 E033 6.5647365 0.009439221 0.26844845 0.50812217 19 40554147 40554425 279 + 0.535 0.777 1.037
ENSG00000160460 E034 6.4701951 0.005999572 0.65504338 0.80673079 19 40554516 40554646 131 + 0.627 0.740 0.474
ENSG00000160460 E035 3.5717173 0.127703401 0.47079121 0.68067305 19 40554647 40555302 656 + 0.632 0.497 -0.615
ENSG00000160460 E036 11.1498353 0.013804669 0.60708954 0.77495046 19 40556084 40556288 205 + 0.959 0.927 -0.120
ENSG00000160460 E037 16.9502634 0.002233876 0.15551857 0.37019432 19 40557023 40557403 381 + 1.192 1.086 -0.382
ENSG00000160460 E038 9.6118991 0.003748384 0.08740616 0.25900049 19 40560159 40560403 245 + 1.030 0.847 -0.688
ENSG00000160460 E039 1.9163318 0.010502377 0.67829055 0.82163230 19 40560404 40560752 349 + 0.418 0.359 -0.336
ENSG00000160460 E040 3.2721968 0.009258101 0.05084554 0.18170753 19 40565423 40565561 139 + 0.768 0.466 -1.339
ENSG00000160460 E041 2.0757223 0.010646811 0.08571757 0.25589000 19 40565661 40565745 85 + 0.627 0.334 -1.487
ENSG00000160460 E042 2.8078985 0.009218571 0.60828540 0.77579632 19 40566163 40566359 197 + 0.535 0.466 -0.336
ENSG00000160460 E043 3.4752231 0.018527538 0.19517025 0.42358158 19 40567663 40568282 620 + 0.257 0.580 1.792
ENSG00000160460 E044 0.3503582 0.025908689 0.16100576   19 40569657 40569726 70 + 0.258 0.053 -2.655
ENSG00000160460 E045 1.8213604 0.013160970 0.72380325 0.85043477 19 40570436 40570728 293 + 0.258 0.359 0.668
ENSG00000160460 E046 0.5061644 0.241576389 0.34402895   19 40571039 40571045 7 + 0.258 0.100 -1.641
ENSG00000160460 E047 3.4277427 0.010161722 0.87193508 0.93697154 19 40571046 40571093 48 + 0.535 0.536 0.005
ENSG00000160460 E048 0.5474829 0.072468461 1.00000000   19 40571544 40571639 96 + 0.000 0.143 11.637
ENSG00000160460 E049 1.7022587 0.097489359 0.53660247 0.72676366 19 40571640 40572018 379 + 0.417 0.311 -0.627
ENSG00000160460 E050 7.2294786 0.006530505 0.77151959 0.87951193 19 40572019 40572192 174 + 0.703 0.788 0.344
ENSG00000160460 E051 0.1723744 0.032318203 0.79243781   19 40572193 40572337 145 + 0.000 0.053 10.334
ENSG00000160460 E052 5.5975559 0.110160078 0.94489105 0.97475539 19 40572338 40572380 43 + 0.628 0.700 0.305
ENSG00000160460 E053 1.2967903 0.305266333 0.35550331   19 40572381 40572632 252 + 0.416 0.250 -1.044
ENSG00000160460 E054 32.7073086 0.086975165 0.14290401 0.35181265 19 40575411 40576464 1054 + 1.139 1.396 0.903