ENSG00000158636

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000334736 ENSG00000158636 HEK293_OSMI2_6hA HEK293_TMG_6hB EMSY protein_coding protein_coding 6.862643 4.818073 10.98197 1.157618 0.766916 1.18693 0.20505058 0.39170599 0.1156709 0.19590178 0.1156709 -1.67648910 0.03139583 0.103600000 0.01213333 -0.09146667 5.769296e-01 9.387068e-15 FALSE TRUE
ENST00000531641 ENSG00000158636 HEK293_OSMI2_6hA HEK293_TMG_6hB EMSY protein_coding retained_intron 6.862643 4.818073 10.98197 1.157618 0.766916 1.18693 0.09251361 0.41229821 0.0000000 0.30649403 0.0000000 -5.40019024 0.02225000 0.079066667 0.00000000 -0.07906667 1.545894e-01 9.387068e-15 FALSE TRUE
ENST00000533988 ENSG00000158636 HEK293_OSMI2_6hA HEK293_TMG_6hB EMSY protein_coding protein_coding 6.862643 4.818073 10.98197 1.157618 0.766916 1.18693 1.23294505 0.28925252 1.8904951 0.12018425 0.2578758 2.66693999 0.16109583 0.059233333 0.17050000 0.11126667 4.080795e-02 9.387068e-15   FALSE
ENST00000534573 ENSG00000158636 HEK293_OSMI2_6hA HEK293_TMG_6hB EMSY protein_coding retained_intron 6.862643 4.818073 10.98197 1.157618 0.766916 1.18693 0.87005788 0.74179866 1.5595679 0.22042812 0.1101052 1.06194916 0.12455833 0.159033333 0.14206667 -0.01696667 9.406737e-01 9.387068e-15 FALSE FALSE
ENST00000695367 ENSG00000158636 HEK293_OSMI2_6hA HEK293_TMG_6hB EMSY protein_coding protein_coding 6.862643 4.818073 10.98197 1.157618 0.766916 1.18693 0.49555603 0.68623932 0.0000000 0.34324612 0.0000000 -6.12151138 0.08607083 0.118500000 0.00000000 -0.11850000 1.323456e-01 9.387068e-15 FALSE TRUE
MSTRG.6025.11 ENSG00000158636 HEK293_OSMI2_6hA HEK293_TMG_6hB EMSY protein_coding   6.862643 4.818073 10.98197 1.157618 0.766916 1.18693 1.03450570 0.39858959 2.0730740 0.25557810 0.2281158 2.34998973 0.14145833 0.084100000 0.19366667 0.10956667 6.437374e-01 9.387068e-15 FALSE TRUE
MSTRG.6025.12 ENSG00000158636 HEK293_OSMI2_6hA HEK293_TMG_6hB EMSY protein_coding   6.862643 4.818073 10.98197 1.157618 0.766916 1.18693 1.19003324 0.02051021 2.7745667 0.02051021 0.1416916 6.51201684 0.12304583 0.003966667 0.25356667 0.24960000 9.387068e-15 9.387068e-15 FALSE TRUE
MSTRG.6025.8 ENSG00000158636 HEK293_OSMI2_6hA HEK293_TMG_6hB EMSY protein_coding   6.862643 4.818073 10.98197 1.157618 0.766916 1.18693 0.68775936 1.35292005 1.2961516 0.46981680 0.7051834 -0.06137853 0.11845000 0.284466667 0.10986667 -0.17460000 6.761552e-01 9.387068e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000158636 E001 2.5763882 0.0096941267 3.456356e-03 2.608287e-02 11 76444923 76445017 95 + 0.660 0.158 -3.030
ENSG00000158636 E002 1.6642372 0.2144711784 1.111549e-01 3.008855e-01 11 76445018 76445021 4 + 0.505 0.156 -2.350
ENSG00000158636 E003 2.1816206 0.2748115092 7.459675e-02 2.339737e-01 11 76445022 76445024 3 + 0.598 0.155 -2.786
ENSG00000158636 E004 2.1462714 0.1354910803 3.382583e-02 1.384027e-01 11 76445025 76445026 2 + 0.592 0.156 -2.746
ENSG00000158636 E005 4.4457447 0.0283228273 1.016084e-01 2.846727e-01 11 76445027 76445041 15 + 0.780 0.559 -0.935
ENSG00000158636 E006 4.8618822 0.0069701485 9.057732e-02 2.648390e-01 11 76445042 76445043 2 + 0.811 0.609 -0.839
ENSG00000158636 E007 5.1659325 0.0058773438 5.353123e-02 1.879398e-01 11 76445044 76445046 3 + 0.842 0.609 -0.959
ENSG00000158636 E008 25.0257651 0.0016133270 1.046069e-01 2.899643e-01 11 76445047 76445128 82 + 1.419 1.351 -0.234
ENSG00000158636 E009 25.1002193 0.0068161311 8.981733e-02 2.634605e-01 11 76446900 76446938 39 + 1.426 1.335 -0.318
ENSG00000158636 E010 31.1629886 0.0012355448 2.122928e-03 1.789146e-02 11 76446939 76447008 70 + 1.538 1.384 -0.532
ENSG00000158636 E011 36.8431236 0.0011652946 1.714364e-05 3.352199e-04 11 76451858 76451957 100 + 1.627 1.407 -0.755
ENSG00000158636 E012 32.2745871 0.0013840798 3.062897e-04 3.793119e-03 11 76453314 76453388 75 + 1.564 1.376 -0.649
ENSG00000158636 E013 12.5863676 0.0030436146 2.068678e-04 2.750321e-03 11 76453389 76453931 543 + 1.222 0.878 -1.259
ENSG00000158636 E014 3.2499719 0.0081331018 1.404207e-01 3.481670e-01 11 76454749 76454790 42 + 0.497 0.764 1.166
ENSG00000158636 E015 0.2027342 0.0350720524 2.997355e-01   11 76458161 76458182 22 + 0.000 0.158 10.815
ENSG00000158636 E016 33.3820390 0.0011817880 8.297248e-02 2.506874e-01 11 76458183 76458358 176 + 1.544 1.488 -0.194
ENSG00000158636 E017 0.4952057 0.3599175162 1.000000e+00   11 76458359 76459932 1574 + 0.170 0.159 -0.113
ENSG00000158636 E018 6.6806226 0.0053466439 8.997729e-01 9.517772e-01 11 76459933 76459935 3 + 0.870 0.902 0.122
ENSG00000158636 E019 27.4192645 0.0089684282 4.131096e-01 6.384637e-01 11 76459936 76460085 150 + 1.451 1.433 -0.062
ENSG00000158636 E020 0.1779838 0.0437880531 7.932164e-01   11 76460086 76461015 930 + 0.093 0.000 -10.510
ENSG00000158636 E021 62.6407783 0.0008582102 8.023622e-06 1.734244e-04 11 76463821 76464080 260 + 1.838 1.675 -0.549
ENSG00000158636 E022 62.9358711 0.0105411355 1.180696e-02 6.566971e-02 11 76472564 76472840 277 + 1.827 1.706 -0.410
ENSG00000158636 E023 56.6206870 0.0007970391 2.261812e-01 4.608261e-01 11 76496215 76496469 255 + 1.748 1.742 -0.019
ENSG00000158636 E024 0.4632531 0.0250399764 1.982380e-01   11 76496470 76497231 762 + 0.234 0.000 -12.184
ENSG00000158636 E025 21.5760490 0.0166744551 2.295438e-01 4.646022e-01 11 76513386 76513396 11 + 1.370 1.302 -0.237
ENSG00000158636 E026 43.5420636 0.0064812782 1.275862e-01 3.280257e-01 11 76513397 76513535 139 + 1.652 1.600 -0.179
ENSG00000158636 E027 46.2844446 0.0009024906 3.059196e-01 5.465889e-01 11 76516142 76516312 171 + 1.658 1.655 -0.009
ENSG00000158636 E028 1.8388792 0.0137435195 4.200829e-01 6.437227e-01 11 76516313 76516842 530 + 0.385 0.559 0.878
ENSG00000158636 E029 43.3059434 0.0016742056 4.561507e-02 1.690672e-01 11 76523155 76523291 137 + 1.649 1.587 -0.211
ENSG00000158636 E030 22.1095816 0.0022266675 8.425761e-01 9.209095e-01 11 76526462 76526478 17 + 1.317 1.384 0.232
ENSG00000158636 E031 48.7779291 0.0008192453 3.131042e-01 5.533675e-01 11 76526479 76526635 157 + 1.682 1.683 0.004
ENSG00000158636 E032 53.9893853 0.0008683445 3.713843e-01 6.050538e-01 11 76528268 76528466 199 + 1.725 1.735 0.034
ENSG00000158636 E033 43.0863165 0.0012719815 1.783397e-01 4.016895e-01 11 76535895 76536059 165 + 1.641 1.619 -0.075
ENSG00000158636 E034 46.5760478 0.0008589387 9.247307e-01 9.647014e-01 11 76537795 76537950 156 + 1.644 1.702 0.198
ENSG00000158636 E035 24.1034676 0.0150570447 4.989889e-01 7.000904e-01 11 76539599 76539640 42 + 1.339 1.448 0.378
ENSG00000158636 E036 8.0999807 0.0091881202 1.651936e-01 3.842074e-01 11 76542051 76542167 117 + 0.981 0.853 -0.485
ENSG00000158636 E037 6.8342177 0.0043542526 1.676013e-01 3.873773e-01 11 76542168 76542215 48 + 0.923 0.796 -0.492
ENSG00000158636 E038 25.9448902 0.0022544524 6.336624e-01 7.927735e-01 11 76542216 76542238 23 + 1.382 1.468 0.298
ENSG00000158636 E039 48.7950038 0.0011977293 6.697473e-01 8.161199e-01 11 76542239 76542367 129 + 1.656 1.731 0.256
ENSG00000158636 E040 94.9337761 0.0060888803 1.745821e-01 3.966029e-01 11 76544259 76544822 564 + 1.923 2.045 0.410
ENSG00000158636 E041 5.2680858 0.0057415116 2.531534e-01 4.913858e-01 11 76544823 76544868 46 + 0.811 0.694 -0.473
ENSG00000158636 E042 33.3673558 0.6209203284 2.456353e-01 4.831425e-01 11 76545797 76545940 144 + 1.374 1.698 1.109
ENSG00000158636 E043 17.1524682 0.4611049500 3.255285e-01 5.647647e-01 11 76545941 76545947 7 + 1.125 1.398 0.962
ENSG00000158636 E044 71.5809904 0.8635756010 2.518923e-01 4.899419e-01 11 76545948 76546297 350 + 1.680 2.037 1.203
ENSG00000158636 E045 0.8380019 0.0491648079 6.427525e-02   11 76547028 76547100 73 + 0.341 0.000 -12.702
ENSG00000158636 E046 273.7764116 0.0040235904 3.257370e-09 1.702081e-07 11 76549952 76553031 3080 + 2.317 2.560 0.810