ENSG00000158615

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367188 ENSG00000158615 HEK293_OSMI2_6hA HEK293_TMG_6hB PPP1R15B protein_coding protein_coding 24.86607 12.24404 40.12936 1.691083 0.7161863 1.71176 21.777718 9.884076 37.148929 1.6006607 0.6365985 1.909072 0.85405417 0.8009000 0.92576667 0.1248667 0.0003907761 0.0003542646 FALSE TRUE
ENST00000687263 ENSG00000158615 HEK293_OSMI2_6hA HEK293_TMG_6hB PPP1R15B protein_coding retained_intron 24.86607 12.24404 40.12936 1.691083 0.7161863 1.71176 1.517227 1.610693 1.411389 0.1022906 0.2448382 -0.189309 0.08305417 0.1368333 0.03496667 -0.1018667 0.0003542646 0.0003542646   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000158615 E001 118.160761 1.2039553290 8.755516e-02 2.591752e-01 1 204396492 204398889 2398 - 1.629 2.459 2.785
ENSG00000158615 E002 25.740476 0.5966996066 8.460580e-02 2.539595e-01 1 204398890 204399550 661 - 1.134 1.721 2.034
ENSG00000158615 E003 12.190641 0.0132807761 5.437264e-06 1.240259e-04 1 204400653 204400789 137 - 0.845 1.391 1.976
ENSG00000158615 E004 14.374010 0.0689184124 1.527862e-01 3.663484e-01 1 204400790 204401599 810 - 1.032 1.310 0.990
ENSG00000158615 E005 6.547982 0.0045003123 2.863901e-03 2.258473e-02 1 204401830 204401902 73 - 0.667 1.095 1.650
ENSG00000158615 E006 2.363329 0.1481593017 1.797312e-01 4.036106e-01 1 204403381 204403414 34 - 0.355 0.709 1.700
ENSG00000158615 E007 151.240786 0.0097606180 4.407322e-03 3.142037e-02 1 204403415 204403664 250 - 2.047 2.280 0.780
ENSG00000158615 E008 292.661305 0.0188757456 4.685187e-01 6.790294e-01 1 204403665 204403849 185 - 2.366 2.498 0.440
ENSG00000158615 E009 1626.235434 0.0051064673 9.207178e-01 9.625855e-01 1 204403850 204406313 2464 - 3.129 3.197 0.226
ENSG00000158615 E010 200.771625 0.0003443036 3.744071e-04 4.482407e-03 1 204409492 204409581 90 - 2.248 2.212 -0.122
ENSG00000158615 E011 119.118230 0.0004323933 2.457098e-04 3.171722e-03 1 204409582 204409587 6 - 2.031 1.958 -0.245
ENSG00000158615 E012 439.450271 0.0001851634 1.163539e-15 1.933421e-13 1 204409588 204410078 491 - 2.599 2.509 -0.300
ENSG00000158615 E013 886.161571 0.0142338758 3.370267e-02 1.380735e-01 1 204410079 204411887 1809 - 2.893 2.853 -0.132