ENSG00000158470

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371711 ENSG00000158470 HEK293_OSMI2_6hA HEK293_TMG_6hB B4GALT5 protein_coding protein_coding 27.44877 21.67215 32.75063 1.547296 1.421202 0.5954555 23.416820 21.3282209 25.323299 1.5053132 0.8716161 0.2475951 0.87931250 0.984233333 0.7744000 -0.2098333 3.067975e-10 3.067975e-10 FALSE TRUE
MSTRG.20826.5 ENSG00000158470 HEK293_OSMI2_6hA HEK293_TMG_6hB B4GALT5 protein_coding   27.44877 21.67215 32.75063 1.547296 1.421202 0.5954555 2.724985 0.1912134 7.092158 0.1029303 1.0746807 5.1414590 0.08079583 0.008866667 0.2150333 0.2061667 6.714755e-06 3.067975e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000158470 E001 0.6376527 0.0193874923 0.908099464   20 49632065 49632105 41 - 0.208 0.225 0.148
ENSG00000158470 E002 1494.0116210 1.8777414982 0.450655322 0.66615222 20 49632945 49635826 2882 - 3.027 3.292 0.883
ENSG00000158470 E003 179.7094944 1.0833767035 0.484453742 0.69017884 20 49635827 49635980 154 - 2.165 2.338 0.580
ENSG00000158470 E004 113.6170872 0.6766361535 0.426209969 0.64817611 20 49635981 49635995 15 - 1.972 2.137 0.553
ENSG00000158470 E005 167.6394927 1.0469091547 0.461755409 0.67435109 20 49635996 49636086 91 - 2.126 2.314 0.631
ENSG00000158470 E006 350.0005330 1.3589743652 0.549986640 0.73624059 20 49636087 49636459 373 - 2.471 2.613 0.476
ENSG00000158470 E007 193.3570833 0.0004285613 0.002916836 0.02290087 20 49637341 49637442 102 - 2.277 2.300 0.077
ENSG00000158470 E008 233.0542842 0.0587537856 0.105645067 0.29176091 20 49639678 49639800 123 - 2.381 2.356 -0.083
ENSG00000158470 E009 259.4329633 0.0856205448 0.183498600 0.40846069 20 49640478 49640665 188 - 2.424 2.405 -0.062
ENSG00000158470 E010 167.1540046 0.0984042095 0.234546956 0.47035967 20 49642468 49642584 117 - 2.235 2.215 -0.067
ENSG00000158470 E011 204.8624850 0.1014839657 0.072189471 0.22919539 20 49643526 49643650 125 - 2.361 2.254 -0.358
ENSG00000158470 E012 172.4552158 0.1052004612 0.046102870 0.17026570 20 49646965 49647078 114 - 2.302 2.158 -0.483
ENSG00000158470 E013 1.6531905 0.0167151912 0.684529134 0.82563145 20 49649497 49649576 80 - 0.348 0.483 0.734
ENSG00000158470 E014 134.1335257 0.1056148749 0.068051905 0.22070505 20 49656568 49656702 135 - 2.189 2.057 -0.439
ENSG00000158470 E015 0.4161375 0.0557707410 0.511487231   20 49679409 49679467 59 - 0.207 0.127 -0.853
ENSG00000158470 E016 53.2624640 0.0735323139 0.088869958 0.26168507 20 49713576 49713904 329 - 1.772 1.689 -0.282