ENSG00000158435

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000289382 ENSG00000158435 HEK293_OSMI2_6hA HEK293_TMG_6hB CNOT11 protein_coding protein_coding 55.01405 40.64223 73.94938 6.253833 2.10453 0.8633986 29.172846 23.365138 28.918463 5.1105594 0.8858189 0.3075148 0.53829583 0.5617000 0.39100000 -0.17070000 1.624459e-01 6.041107e-06 FALSE TRUE
ENST00000420107 ENSG00000158435 HEK293_OSMI2_6hA HEK293_TMG_6hB CNOT11 protein_coding protein_coding 55.01405 40.64223 73.94938 6.253833 2.10453 0.8633986 13.568310 8.832761 20.786756 1.6206527 0.2771958 1.2337897 0.24780000 0.2200333 0.28176667 0.06173333 6.023429e-01 6.041107e-06 FALSE TRUE
ENST00000462489 ENSG00000158435 HEK293_OSMI2_6hA HEK293_TMG_6hB CNOT11 protein_coding retained_intron 55.01405 40.64223 73.94938 6.253833 2.10453 0.8633986 8.967302 4.577385 22.083554 0.5048373 1.9735063 2.2678816 0.15106667 0.1221667 0.29760000 0.17543333 3.894512e-02 6.041107e-06 FALSE FALSE
MSTRG.18948.1 ENSG00000158435 HEK293_OSMI2_6hA HEK293_TMG_6hB CNOT11 protein_coding   55.01405 40.64223 73.94938 6.253833 2.10453 0.8633986 3.223033 3.823348 1.797966 0.6822915 0.1686506 -1.0842375 0.06138333 0.0949000 0.02446667 -0.07043333 6.041107e-06 6.041107e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000158435 E001 10.29542 0.0044700845 9.369936e-08 3.500287e-06 2 101252795 101252885 91 + 0.682 1.274 2.226
ENSG00000158435 E002 14.40584 0.0214301303 3.767263e-05 6.552984e-04 2 101252886 101252901 16 + 0.905 1.372 1.683
ENSG00000158435 E003 257.06835 0.0020936258 9.970027e-09 4.696643e-07 2 101252902 101253478 577 + 2.325 2.499 0.582
ENSG00000158435 E004 94.99483 0.0068203379 4.750864e-01 6.836380e-01 2 101257791 101257837 47 + 1.965 2.009 0.146
ENSG00000158435 E005 224.29463 0.0028354179 5.783115e-01 7.560218e-01 2 101257838 101257955 118 + 2.363 2.343 -0.065
ENSG00000158435 E006 2.00096 0.0169766743 7.351534e-01 8.573525e-01 2 101262431 101262538 108 + 0.440 0.497 0.285
ENSG00000158435 E007 399.08753 0.0011410406 1.008314e-01 2.832763e-01 2 101262539 101262691 153 + 2.619 2.582 -0.124
ENSG00000158435 E008 448.82097 0.0002429374 1.016627e-02 5.883742e-02 2 101264845 101264974 130 + 2.672 2.629 -0.143
ENSG00000158435 E009 360.64612 0.0002056246 1.849288e-02 9.069557e-02 2 101264975 101265047 73 + 2.578 2.535 -0.142
ENSG00000158435 E010 537.91319 0.0006642652 3.292791e-01 5.682837e-01 2 101266677 101266879 203 + 2.738 2.721 -0.056
ENSG00000158435 E011 312.98692 0.0009479092 1.123858e-01 3.031206e-01 2 101269040 101269136 97 + 2.477 2.514 0.126
ENSG00000158435 E012 69.38584 0.0273218011 5.999806e-03 3.972494e-02 2 101269137 101269215 79 + 1.945 1.684 -0.878
ENSG00000158435 E013 1266.82848 0.0027435508 2.530978e-01 4.913556e-01 2 101269216 101270316 1101 + 3.090 3.114 0.079
ENSG00000158435 E014 52.33778 0.6904859162 7.389114e-01 8.597573e-01 2 101273500 101288876 15377 + 1.904 1.324 -1.978