ENSG00000157916

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000378518 ENSG00000157916 HEK293_OSMI2_6hA HEK293_TMG_6hB RER1 protein_coding protein_coding 155.7879 172.5012 110.8951 7.008174 3.33958 -0.6373642 30.06049 41.220714 14.77438 2.167128 0.5927621 -1.4796461 0.19085417 0.23953333 0.13316667 -0.10636667 0.0001452144 0.0001452144 FALSE FALSE
ENST00000443438 ENSG00000157916 HEK293_OSMI2_6hA HEK293_TMG_6hB RER1 protein_coding protein_coding 155.7879 172.5012 110.8951 7.008174 3.33958 -0.6373642 75.99936 79.035788 59.10476 4.006185 2.9488692 -0.4191702 0.48739167 0.45786667 0.53233333 0.07446667 0.1126321528 0.0001452144 FALSE FALSE
ENST00000605895 ENSG00000157916 HEK293_OSMI2_6hA HEK293_TMG_6hB RER1 protein_coding protein_coding 155.7879 172.5012 110.8951 7.008174 3.33958 -0.6373642 16.47680 16.470485 11.52000 4.323888 1.2774033 -0.5153660 0.10429167 0.09403333 0.10470000 0.01066667 0.9339744160 0.0001452144 FALSE TRUE
MSTRG.144.3 ENSG00000157916 HEK293_OSMI2_6hA HEK293_TMG_6hB RER1 protein_coding   155.7879 172.5012 110.8951 7.008174 3.33958 -0.6373642 8.93683 9.322962 4.27924 1.193195 0.3544334 -1.1216131 0.05619583 0.05383333 0.03876667 -0.01506667 0.5726671118 0.0001452144 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000157916 E001 0.4054685 0.5462326383 5.735814e-01   1 2391775 2391786 12 + 0.000 0.177 10.494
ENSG00000157916 E002 0.5474829 0.0255875528 2.310191e-01   1 2391787 2391793 7 + 0.000 0.233 11.011
ENSG00000157916 E003 27.3340049 0.0015342759 3.160366e-05 0.0005648314 1 2391794 2391827 34 + 1.191 1.514 1.124
ENSG00000157916 E004 54.6437803 0.0217746116 1.250601e-04 0.0018004483 1 2391828 2391840 13 + 1.461 1.818 1.214
ENSG00000157916 E005 58.8021743 0.0189643775 6.672351e-05 0.0010652615 1 2391841 2391842 2 + 1.498 1.848 1.188
ENSG00000157916 E006 98.5280118 0.0300406270 3.502748e-03 0.0263626617 1 2391843 2391856 14 + 1.780 2.050 0.909
ENSG00000157916 E007 232.1327677 0.0226462267 6.603501e-04 0.0071012309 1 2391857 2391889 33 + 2.156 2.418 0.875
ENSG00000157916 E008 389.9111731 0.0056924315 1.965384e-05 0.0003768762 1 2391890 2391958 69 + 2.443 2.622 0.596
ENSG00000157916 E009 5.4291302 0.0796682608 6.758925e-01 0.8201239751 1 2392039 2392126 88 + 0.729 0.815 0.344
ENSG00000157916 E010 36.9695227 0.0433003439 7.593260e-01 0.8721409040 1 2392259 2392375 117 + 1.557 1.559 0.005
ENSG00000157916 E011 50.5931552 0.0581238551 1.745586e-01 0.3965867591 1 2392457 2392562 106 + 1.771 1.642 -0.438
ENSG00000157916 E012 82.2389318 0.0408170905 2.359894e-01 0.4719537574 1 2392563 2392715 153 + 1.950 1.868 -0.277
ENSG00000157916 E013 96.1249624 0.0357668249 6.802654e-02 0.2206396541 1 2392991 2393157 167 + 2.061 1.905 -0.524
ENSG00000157916 E014 47.0268423 0.0130864298 1.176151e-02 0.0654898593 1 2393174 2393207 34 + 1.766 1.589 -0.600
ENSG00000157916 E015 54.5479890 0.0126911839 4.994976e-02 0.1795686044 1 2393208 2393231 24 + 1.800 1.673 -0.432
ENSG00000157916 E016 57.7525529 0.0168345667 8.779621e-02 0.2596185149 1 2393232 2393269 38 + 1.818 1.702 -0.392
ENSG00000157916 E017 174.2666213 0.0034002719 8.186635e-01 0.9072381527 1 2393270 2393355 86 + 2.222 2.219 -0.010
ENSG00000157916 E018 117.1296788 0.1026478117 2.270233e-01 0.4617982017 1 2394005 2394646 642 + 2.119 2.009 -0.366
ENSG00000157916 E019 36.8346037 0.0967092872 7.848130e-01 0.8874223491 1 2394647 2394728 82 + 1.569 1.549 -0.071
ENSG00000157916 E020 157.2229123 0.0823477424 5.130366e-01 0.7102769171 1 2394729 2395607 879 + 2.193 2.169 -0.079
ENSG00000157916 E021 24.5985279 0.0074293553 5.340368e-01 0.7249249168 1 2395608 2395648 41 + 1.417 1.370 -0.163
ENSG00000157916 E022 44.3328356 0.0323844165 4.378677e-01 0.6571177735 1 2395649 2395783 135 + 1.672 1.614 -0.196
ENSG00000157916 E023 731.3658296 0.0008625967 8.075673e-02 0.2463640940 1 2395784 2395871 88 + 2.816 2.852 0.120
ENSG00000157916 E024 15.0835097 0.0987046585 3.917479e-01 0.6218249345 1 2395872 2396367 496 + 1.254 1.136 -0.419
ENSG00000157916 E025 853.7976362 0.0014794192 2.963609e-01 0.5368987765 1 2397116 2397220 105 + 2.890 2.916 0.087
ENSG00000157916 E026 964.1326726 0.0008679686 2.230759e-02 0.1036255291 1 2399415 2399514 100 + 2.983 2.947 -0.120
ENSG00000157916 E027 965.0590576 0.0026098865 8.193650e-02 0.2486322102 1 2400857 2400935 79 + 2.987 2.947 -0.133
ENSG00000157916 E028 14.1922623 0.1045157929 5.593665e-01 0.7428605617 1 2401951 2402096 146 + 1.180 1.150 -0.109
ENSG00000157916 E029 28.2302291 0.0237263659 5.171074e-01 0.7130702331 1 2402097 2402206 110 + 1.393 1.473 0.277
ENSG00000157916 E030 1273.6596765 0.0008578490 5.381546e-05 0.0008869472 1 2402207 2402342 136 + 3.120 3.060 -0.200
ENSG00000157916 E031 2427.6545961 0.0101607157 7.165777e-01 0.8459837069 1 2403035 2405442 2408 + 3.350 3.370 0.068