ENSG00000157578

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380671 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding protein_coding 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.041610995 0.02195008 0.03715979 0.02195008 0.03715979 0.5617376 0.063770833 0.03620000 0.04550000 0.00930000 0.99273973 0.02027701 FALSE TRUE
ENST00000434281 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding protein_coding 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.013567591 0.10854073 0.00000000 0.05570317 0.00000000 -3.5673109 0.021175000 0.16940000 0.00000000 -0.16940000 0.02027701 0.02027701   FALSE
ENST00000438404 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding protein_coding 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.008330994 0.04043894 0.00000000 0.04043894 0.00000000 -2.3345379 0.010920833 0.06666667 0.00000000 -0.06666667 0.74241680 0.02027701 TRUE FALSE
ENST00000448288 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding protein_coding 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.005295929 0.04236743 0.00000000 0.04236743 0.00000000 -2.3886698 0.008733333 0.06986667 0.00000000 -0.06986667 0.74241680 0.02027701 FALSE FALSE
ENST00000451131 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding protein_coding 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.021536086 0.00000000 0.05708744 0.00000000 0.02866146 2.7460427 0.028520833 0.00000000 0.10246667 0.10246667 0.61705156 0.02027701   FALSE
ENST00000456017 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding protein_coding 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.011988493 0.00000000 0.06164058 0.00000000 0.06164058 2.8407771 0.032512500 0.00000000 0.05703333 0.05703333 0.90038038 0.02027701   FALSE
ENST00000459939 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding processed_transcript 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.047344786 0.07557595 0.04512726 0.07557595 0.04512726 -0.6344395 0.042687500 0.11283333 0.04173333 -0.07110000 0.93064451 0.02027701 FALSE FALSE
ENST00000480612 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding retained_intron 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.028225797 0.08573550 0.00000000 0.02665664 0.00000000 -3.2590540 0.069983333 0.28176667 0.00000000 -0.28176667 0.06356612 0.02027701   FALSE
ENST00000484878 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding processed_transcript 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.003270408 0.00000000 0.02616327 0.00000000 0.02616327 1.8545249 0.007941667 0.00000000 0.06353333 0.06353333 0.95568729 0.02027701   FALSE
ENST00000485895 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding protein_coding 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.204783453 0.06661427 0.21865214 0.01994269 0.01700200 1.5774693 0.387470833 0.22070000 0.34836667 0.12766667 0.79625461 0.02027701 FALSE TRUE
ENST00000490184 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding processed_transcript 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.042981606 0.00000000 0.07100876 0.00000000 0.07100876 3.0180780 0.039929167 0.00000000 0.08696667 0.08696667 0.91144912 0.02027701   FALSE
ENST00000491625 ENSG00000157578 HEK293_OSMI2_6hA HEK293_TMG_6hB LCA5L protein_coding retained_intron 0.6758904 0.4675261 0.769825 0.1715688 0.1945514 0.7075708 0.157633878 0.02111437 0.16789258 0.02111437 0.08827532 2.5153535 0.169125000 0.03480000 0.17573333 0.14093333 0.69762185 0.02027701 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000157578 E001 0.1779838 0.168107234 1.000000000   21 39405728 39405843 116 - 0.097 0.000 -9.959
ENSG00000157578 E002 0.1779838 0.168107234 1.000000000   21 39405844 39405859 16 - 0.097 0.000 -12.333
ENSG00000157578 E003 0.5227326 0.046772861 0.306753313   21 39405860 39405977 118 - 0.096 0.265 1.760
ENSG00000157578 E004 0.9927399 0.106453777 0.003459682   21 39405978 39406612 635 - 0.000 0.548 14.683
ENSG00000157578 E005 0.0000000       21 39409979 39410096 118 -      
ENSG00000157578 E006 0.1426347 0.071237043 1.000000000   21 39410264 39410367 104 - 0.096 0.000 -12.347
ENSG00000157578 E007 0.0000000       21 39411033 39411147 115 -      
ENSG00000157578 E008 0.1426347 0.071237043 1.000000000   21 39411718 39411802 85 - 0.096 0.000 -12.347
ENSG00000157578 E009 0.5893196 0.138389626 0.184056952   21 39420706 39420843 138 - 0.297 0.000 -13.626
ENSG00000157578 E010 3.6896578 0.010547611 0.244269641 0.48139307 21 39421560 39422411 852 - 0.718 0.546 -0.746
ENSG00000157578 E011 5.7194704 0.009073328 0.015513781 0.08001327 21 39422412 39422975 564 - 0.913 0.595 -1.292
ENSG00000157578 E012 0.7507353 0.153581431 0.114654807   21 39422976 39422987 12 - 0.347 0.000 -13.813
ENSG00000157578 E013 1.2210030 0.019946467 0.130282009   21 39422988 39423083 96 - 0.437 0.152 -2.049
ENSG00000157578 E014 1.6938594 0.018021656 0.492101975 0.69541818 21 39423084 39423239 156 - 0.475 0.353 -0.663
ENSG00000157578 E015 0.4632531 0.024415389 0.255807822   21 39423240 39423244 5 - 0.241 0.000 -13.413
ENSG00000157578 E016 2.5912232 0.018382647 0.871560415 0.93680222 21 39423245 39423419 175 - 0.572 0.544 -0.124
ENSG00000157578 E017 1.6341198 0.073416634 1.000000000 1.00000000 21 39423420 39423490 71 - 0.435 0.425 -0.057
ENSG00000157578 E018 1.1308332 0.017267182 0.644164145   21 39427759 39427905 147 - 0.350 0.265 -0.561
ENSG00000157578 E019 0.2852693 0.342752174 0.623572647   21 39428172 39428207 36 - 0.172 0.000 -12.959
ENSG00000157578 E020 0.2852693 0.342752174 0.623572647   21 39428208 39428209 2 - 0.172 0.000 -12.959
ENSG00000157578 E021 0.2852693 0.342752174 0.623572647   21 39428210 39428214 5 - 0.172 0.000 -12.959
ENSG00000157578 E022 0.2852693 0.342752174 0.623572647   21 39428215 39428221 7 - 0.172 0.000 -12.959
ENSG00000157578 E023 0.4161375 0.118139535 1.000000000   21 39428222 39428284 63 - 0.174 0.151 -0.241
ENSG00000157578 E024 0.4349185 0.024691078 1.000000000   21 39428285 39428304 20 - 0.175 0.152 -0.243
ENSG00000157578 E025 0.9520415 0.019387492 0.149875091   21 39428305 39428401 97 - 0.175 0.428 1.760
ENSG00000157578 E026 0.6370325 0.021768165 0.127295781   21 39428402 39428461 60 - 0.096 0.354 2.343
ENSG00000157578 E027 0.4756169 0.024441170 0.028743421   21 39428462 39428503 42 - 0.000 0.354 13.971
ENSG00000157578 E028 0.0000000       21 39428504 39428522 19 -      
ENSG00000157578 E029 1.4795758 0.039190358 0.018777371 0.09164883 21 39429131 39429187 57 - 0.175 0.595 2.562
ENSG00000157578 E030 1.1348270 0.017401120 0.069437136   21 39429188 39429211 24 - 0.175 0.491 2.078
ENSG00000157578 E031 0.6536007 0.021768165 0.126218400   21 39429212 39429394 183 - 0.096 0.354 2.342
ENSG00000157578 E032 1.4451070 0.017544973 0.830634986 0.91414556 21 39435420 39435542 123 - 0.396 0.354 -0.244
ENSG00000157578 E033 0.4349185 0.024691078 1.000000000   21 39435543 39435573 31 - 0.175 0.152 -0.243
ENSG00000157578 E034 0.1723744 0.035065975 0.268060707   21 39436097 39436167 71 - 0.000 0.152 12.732
ENSG00000157578 E035 0.0000000       21 39438684 39438718 35 -      
ENSG00000157578 E036 0.0000000       21 39438719 39438936 218 -      
ENSG00000157578 E037 0.3503582 0.028799113 0.691648129   21 39439634 39439773 140 - 0.096 0.152 0.759
ENSG00000157578 E038 0.5059767 0.027442404 0.027665574   21 39443459 39444134 676 - 0.000 0.354 13.971
ENSG00000157578 E039 1.8667111 0.018543697 0.579155295 0.75659748 21 39444135 39444201 67 - 0.396 0.492 0.495
ENSG00000157578 E040 0.4873834 0.036431971 0.300712639   21 39444644 39444745 102 - 0.096 0.265 1.764
ENSG00000157578 E041 0.3503582 0.028799113 0.691648129   21 39445450 39445724 275 - 0.096 0.152 0.759
ENSG00000157578 E042 0.9680622 0.244564401 0.554299554   21 39445725 39445805 81 - 0.246 0.349 0.695